Effect and mechanism of peanut skin proanthocyanidins on gliadin-induced Caco-2 celiac disease model cells

Wang, Na; Cui, Chenxu; Xu, Chao; Ren, Hongtao; Wang, Fan; Yu, Qiuying; Zhang, Gaiping

doi:10.1016/j.clim.2022.109100

Cited by 10 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussionmentioning

confidence: 99%

“…In our study, the constituents of PC and RES were performed by extensive spectrometry analysis. The results showed that procyanidin A was the main component of PC extract (Wang et al, 2022 ), and the main components of RES extract were oxidized resveratrol, resveratrol glycosides, resveratrol dimer, and tetramer (Appendix S1 ). We speculated that the inhibitory effect of PC on the growth and proliferation of CACO‐2 colorectal cancer cells might attribute to the stronger antioxidant capacity of procyanidin A but the result of which needs to be further verified.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The combined anticancer of peanut skin procyanidins and resveratrol to CACO‐2 colorectal cancer cells

Wang

Gao

Cui

et al. 2023

Food Science & Nutrition

Self Cite

View full text Add to dashboard Cite

Colorectal cancer is one of the leading causes of cancer deaths worldwide. Currently, chemotherapy is the primary way for colorectal cancer, but with severe side effects. Therefore, it is urgent to find safer and more effective adjuvant treatment methods. At present, natural active substances are promising alternatives, as numerous studies have demonstrated possible synergistic anticancer effects in plant‐active polyphenols. In the present study, the combined effect of procyanidins (PC) (from peanut skin) and resveratrol (RES) (from peanut buds) on the synergistic anticancer potential was investigated. CACO‐2 and HCT‐8 cells were served as colorectal cancer models, and HEPG‐2 and HUH‐7 cells were served as liver cancer models to observe the effects of PC and RES alone or in combination on the growth and proliferation of these four types of cancer cells. The results revealed that both PC and RES could inhibit the cells’ proliferation in a manner with concentration‐dependent, but they exerted synergistic anticancer effects only on CACO‐2 cells. PC and RES could synergistically inhibit CACO‐2 cell clone formation, inducing apoptosis of CACO‐2 cells and blocking their cell cycle in G0/G1 phase. Additionally, as observed by the results of Western blot assay, the combined effect of PC and RES also inhibited the phosphorylation of Thr308, Ser473, and ERK and promoted the phosphorylation of IKBα and NF‐κB in CACO‐2 cells. These findings collectively indicate that PC combined with RES might exert synergistic anticancer effects by regulating AKT, ERK, and NF‐κB signaling pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The combined anticancer of peanut skin procyanidins and resveratrol to CACO‐2 colorectal cancer cells

Wang

Gao

Cui

et al. 2023

Food Science & Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

“…In normal conditions, they can be found in the cytoplasm in an inactive form, but in certain circumstances, such as inflammatory signals, the p65 subunit is translocated into the nucleus, opening the epithelial barrier at the level of signaling pathways that regulate many biological processes [ 34 , 35 ]. Activation is indirectly achieved by various agents; e.g., it is also triggered by the toxins of pathogenic microorganisms and gliadin [ 36 , 37 ]. In our previous studies, TNF-α and IL-1β were used for direct induction; however, in our current studies, Figure 4 shows that NF-κB was also induced by PT-gliadin.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Anthocyanin-Rich Sour Cherry Extract on Gliadin-Induced Caco-2 Barrier Damage

Klusóczki,

Oláh,

Hosszú

et al. 2023

Nutrients

View full text Add to dashboard Cite

Several types of gluten-related disorders are known, in which the common starting point is gluten-induced zonulin release. Zonulin results in varying degrees of increased permeability in certain gluten-related disorders but is largely responsible for the development of further pathogenic processes and symptoms. Therefore, it is important to know the barrier-modulating role of individual nutritional components and to what extent the antioxidant substance supports the protection of gliadin-induced membrane damage with its radical scavenging capacity. We investigated the pH dependence of the gliadin-anthocyanin interaction using UV photometry, during which a concentration-dependent interaction was observed at pH 6.8. The barrier modulatory effect of the anthocyanin-rich sour cherry extract (AC) was analyzed on Caco-2 cell culture with pepsin-trypsin-resistant gliadin (PT-gliadin) exposure by TEER measurement, zonula occludens-1 (ZO-1), and Occludin immunohistochemistry. In addition to the TEER-reducing and TJ-rearranging effects of PT-gliadin, NF-κB activation, an increase in cytokine (TNF-α, IFN-γ, and IL-8) release, and mitochondrial ROS levels were observed. We confirmed the anti-inflammatory, stabilizing, and restoring roles of AC extract during gliadin treatment on the Caco-2 monolayer. The extract was able to significantly reduce cytokine and ROS levels despite the known interaction of the main components of the extract with PT-gliadin.

show abstract

“… 354 The pathogenesis of CD involves innate and adaptive immunity, primarily mediated by the infiltration of lymphocytes, particularly T cells, into the small intestinal epithelium. 355 The upregulation of the NF-κB pathway and its downstream cytokines, such as IL-8, in the intestinal mucosa suggests the involvement of NF-κB in the development of celiac disease. 356 …”

Section: Nf-κb Signaling In Human Diseasesmentioning

confidence: 99%

NF-κB in biology and targeted therapy: new insights and translational implications

Guo,

Jin,

Chen

et al. 2024

Sig Transduct Target Ther

197

View full text Add to dashboard Cite

NF-κB signaling has been discovered for nearly 40 years. Initially, NF-κB signaling was identified as a pivotal pathway in mediating inflammatory responses. However, with extensive and in-depth investigations, researchers have discovered that its role can be expanded to a variety of signaling mechanisms, biological processes, human diseases, and treatment options. In this review, we first scrutinize the research process of NF-κB signaling, and summarize the composition, activation, and regulatory mechanism of NF-κB signaling. We investigate the interaction of NF-κB signaling with other important pathways, including PI3K/AKT, MAPK, JAK-STAT, TGF-β, Wnt, Notch, Hedgehog, and TLR signaling. The physiological and pathological states of NF-κB signaling, as well as its intricate involvement in inflammation, immune regulation, and tumor microenvironment, are also explicated. Additionally, we illustrate how NF-κB signaling is involved in a variety of human diseases, including cancers, inflammatory and autoimmune diseases, cardiovascular diseases, metabolic diseases, neurological diseases, and COVID-19. Further, we discuss the therapeutic approaches targeting NF-κB signaling, including IKK inhibitors, monoclonal antibodies, proteasome inhibitors, nuclear translocation inhibitors, DNA binding inhibitors, TKIs, non-coding RNAs, immunotherapy, and CAR-T. Finally, we provide an outlook for research in the field of NF-κB signaling. We hope to present a stereoscopic, comprehensive NF-κB signaling that will inform future research and clinical practice.

show abstract

Effect and mechanism of peanut skin proanthocyanidins on gliadin-induced Caco-2 celiac disease model cells

Cited by 10 publications

References 24 publications

The combined anticancer of peanut skin procyanidins and resveratrol to CACO‐2 colorectal cancer cells

The combined anticancer of peanut skin procyanidins and resveratrol to CACO‐2 colorectal cancer cells

Effectiveness of Anthocyanin-Rich Sour Cherry Extract on Gliadin-Induced Caco-2 Barrier Damage

NF-κB in biology and targeted therapy: new insights and translational implications

Contact Info

Product

Resources

About