Effect and mechanisms of kaempferol against endometriosis based on network pharmacology and in vitro experiments

Zhao, Junde; Wang, Juntao; Liu, Jin-Xing; Li, Shuling; Liu, Pengfei; Zhang, Xiaodan

doi:10.1186/s12906-022-03729-4

Cited by 6 publications

(7 citation statements)

References 42 publications

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“…The activation of β-catenin triggers the Epithelial-Mesenchymal Transition (EMT) in endometrial epithelial cells via TGF-β2, and inhibiting TGF-β effectively suppresses the EMT-related effects of β-catenin activation [140]. Moreover, it is suggested that kaempferol plays a role in regulating genes associated with PI3K, particularly Phosphatase and Tensin Homologue (PTEN), and MMP9, thereby inhibiting endometrial cell implantation and ectopic formation [141]. Proteins linked to adhesion and implantation have also been detected in endometriotic cells through appropriate staining.…”

Section: Cytoskeletal Proteins: Potential Contributors In Endometriosismentioning

confidence: 99%

Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology

Szymański,

Bonowicz,

Antosik

et al. 2024

Cancers

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Endometriosis is a gynecological condition where endometrium-like tissue grows outside the uterus, posing challenges in understanding and treatment. This article delves into the deep cellular and molecular processes underlying endometriosis, with a focus on the crucial roles played by cyclins and cytoskeletal proteins in its pathogenesis, particularly in the context of Epithelial–Mesenchymal Transition (EMT). The investigation begins by examining the activities of cyclins, elucidating their diverse biological roles such as cell cycle control, proliferation, evasion of apoptosis, and angiogenesis among ectopic endometrial cells. A comprehensive analysis of cytoskeletal proteins follows, emphasizing their fundamental biological roles and their specific significance to endometriotic cell features. This review sheds light on the interconnected pathways through which cyclins and cytoskeletal proteins converge, contributing to the genesis and progression of endometriosis. Understanding these molecular complexities not only provides insight into the underlying causes of the disease but also holds promise for the development of specific therapeutic approaches, ushering in a new era in the management of this devastating disorder.

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Section: Cytoskeletal Proteins: Potential Contributors In Endometriosismentioning

confidence: 99%

Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology

Szymański,

Bonowicz,

Antosik

et al. 2024

Cancers

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“…The keyword "obesity" was searched in three different disease gene databases: (1) GeneCards Human Gene database (https://www.genecards.org/); (2) DisGeNET database (https://www.disgenet.org/home/); (3) Online Mendelian Inheritance in Man (OMIM) database (https://omim.org/). UniProt (http://www.UniProt.org) was used to obtain the gene symbols of all targets (obesity and kudingcha), and the information was used for subsequent network pharmacological data analysis (Zhao et al 2022).…”

Section: Prediction Of Endometriosis Targetsmentioning

confidence: 99%

“…After processing the data by R software (R version 4.1.2), we obtained the top 30 hub genes. Subsequently, we imported the hub gene data into the Cy software and obtained the HUB gene PPI network diagram (Zhao et al 2022).…”

Section: Protein-protein Interaction (Ppi) Network Constructionmentioning

confidence: 99%

Effects and mechanism of Kudingcha on obesity based on network pharmacology

Sui,

Liu,

Gong

et al. 2023

Preprint

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Context: Obesity is the world's most high-profile public health problem. Kudingcha has been used in traditional medicine because of its antioxidant, anti-inflammatory and weight loss properties. Quercetin and kaempferol are the main components rich in Kudingcha that are effective against obesity. Objective We used the method of network pharmacology to study the effects of quercetin and kaempferol to HepG2 cells on obesity. Materials & methods We obtained 20 hub genes through PPI results and the Cytoscape plug-in cytoHubba. The top 10 genes were found eventrully by GO and KEGG enrichment analysis. The results showed that the insulin resistance, PI3K-Akt signaling pathways and adipocytokine signaling pathway might be the central pathways associated with the treatment of obesity by kudingcha. Next, we used Real-Time PCR to detect the expression levels of ISNR, SLc2a4 and APOE genes to study its mechanism. Results The results showed that compared with the blank group, the expression of ISNR and SLc2a4 increased and the expression of APOE decreased. Discussion & Conclusion: Our experiments confirmed that Kudingcha contains compounds that can improve insulin resistance and obesity. This can provide a reference for further research and application of Kudingcha in the treatment of obesity.

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“…(3) Online Mendelian Inheritance in Man (OMIM) database (https://omim.org/). UniProt (http://www.UniProt.org) was used to obtain the gene symbols of all targets (obesity and kudingcha), and the information was used for subsequent network pharmacological data analysis [12].…”

Section: Prediction Of Endometriosis Targetsmentioning

confidence: 99%

Section: Protein-protein Interaction (Ppi) Network Constructionmentioning

confidence: 99%

Effects and mechanism of Kudingcha on obesity

Sui

Liu

Gong

et al. 2022

Preprint

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Obesity is the world's most high-profile public health problem. Kudingcha has been used in traditional medicine because of its antioxidant, anti-inflammatory and weight loss properties. Quercetin and kaempferol are the main components rich in Kudingcha that are effective against obesity. Therefore, we used the method of network pharmacology to study the effects of quercetin and kaempferol to HepG2 cells on obesity. We obtained 20 hub genes through PPI results and the Cytoscape plug-in cytoHubba. The top 10 genes were found eventrully by GO and KEGG enrichment analysis. The results showed that the insulin resistance, PI3K-Akt signaling pathways and adipocytokine signaling pathway might be the central pathways associated with the treatment of obesity by kudingcha. Next, we used Real-Time PCR to detect the expression levels of ISNR, SLc2a4 and APOE genes to study its mechanism. The results showed that compared with the blank group, the expression of ISNR and SLc2a4 increased and the expression of APOE decreased. Our experiments confirmed that Kudingcha contains compounds that can improve insulin resistance and obesity. This can provide a reference for further research and application of Kudingcha in the treatment of obesity.

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Effect and mechanisms of kaempferol against endometriosis based on network pharmacology and in vitro experiments

Cited by 6 publications

References 42 publications

Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology

Role of Cyclins and Cytoskeletal Proteins in Endometriosis: Insights into Pathophysiology

Effects and mechanism of Kudingcha on obesity based on network pharmacology

Effects and mechanism of Kudingcha on obesity

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