Effect-directed analysis and chemical identification of agonists of peroxisome proliferator-activated receptors in white button mushroom

Ngoc, Long Pham; Helmus, Rick; Ehlers, Andreas W.; Swart, Kees; Besselink, H.; Rijke, Eva de; Cam, Ha Dang Thi; Brouwer, Abraham; Burg, Bart van der

doi:10.1039/d0fo02071k

Cited by 4 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MS conditions were as follows: ESI in positive ion mode with a spray voltage of 4000V, endplate offset of -500V, nebulizer gas pressure of 1.2 bar, dry gas flow rate of 8.0 L/min, dry gas temperature of 200 °C, and mass range of 20-800 m/z. Automated multiple reaction monitoring was performed, and auto MS/MS data acquisition used settings similar to others: precursor ions were acquired using a cycle time of 3.0 seconds for 3 ions; mass range was set to 20-800 m/z; ion polarity was positive; active exclusion was set to exclude after 3 spectra, release after 1.0 minute, and reconsider precursor if current intensity / previous intensity equaled 5× (32)(33)(34).…”

Section: Root Metabolite Extraction and Metabolomic Analysismentioning

confidence: 99%

Biochar Modulates Wheat Root Metabolome and Rhizosphere Microbiome in a Feedstock-dependent Manner

Yang,

Kerner,

Liang

et al. 2024

Preprint

View full text Add to dashboard Cite

BackgroundBiochar is a multifunctional soil conditioner capable of enhancing soil health and plant productivity, but the underlying mechanisms remain elusive. Here we tackled this question using wheat as a model plant and through the lens of the rhizosphere, a vital soil-plant interface continuum. We systematically examined the effects of four types of biochar (corn stover, cattle manure, pine sawdust, or wheat straw) applied at two rates (0.25% or 2.5%, w/w).ResultsEmploying untargeted metabolomics and 16S rRNA gene sequencing, we revealed both common and unique modulating effects of the tested biochar treatments on wheat root metabolites and rhizosphere microbiome structure and functioning. Biochar modulated numerous metabolic pathways in wheat roots, where amino acid metabolism was the most common one, leading to cascade effects on the dynamics of a wide range of secondary metabolites, including many plant signaling molecules (e.g., flavonoid compounds, brassinosteroids) that are known to be involved in plant-microbe interactions. All biochar treatments increased rhizosphere microbial diversity, altered community composition, enhanced microbial interactions, and resulted in functional changes. Increased Burkholderiales (denitrifying bacteria) abundance and decreased Thermoplasmata (archaeal methanogens) abundance could explain biochar’s widely reported effects on nitrous oxide and methane mitigation, respectively. Biochar enhanced positive correlations among microbes and network complexity, particularly modularity, suggesting local adaptation through mutualism and/or synergism and the formation of modules of functionally interrelated taxa. A large number of diverse keystone taxa from both dominant and non-dominant phyla emerged after biochar treatments, including those known to be involved in methane, nitrogen, and sulfur cycling. Besides common alterations, treatment-specific alterations also occurred, and biochar type (i.e., feedstock choice) exerted greater influence than application rate. Wheat biochar applied at a 0.25% rate showed the strongest and distinct modulating effects, resulting in orchestrated changes in both root metabolites and rhizosphere microbiome, especially those relevant to plant-microbe interactions and likely beneficial to the host plant (e.g., upregulated biosynthesis of zeatin and down-regulated limonene degradation).ConclusionsOur work contributes to a mechanistic understanding of how biochar modulates the soil-plant continuum and provides new insights into the potential of top-down rhizosphere microbiome engineering through biochar-based reprogramming of root-microbe interactions.

show abstract

Section: Root Metabolite Extraction and Metabolomic Analysismentioning

confidence: 99%

Biochar Modulates Wheat Root Metabolome and Rhizosphere Microbiome in a Feedstock-dependent Manner

Yang,

Kerner,

Liang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Waqas et al identified pentadecanoic acid, 14-methyl-, methyl ester, 1-hexadecanol, 2-methyl-, 9, 12-octadecadienoic acid (Z, Z)-, methyl ester from A. blazei substrates by GCMS [23]. In 2021, Pham et al isolated (9Z,12Z)-9,12-octadecanoic acid from A. bisporus substrates in their search for anti-peroxisome proliferator-activated receptor agonists (PPARs) [24].In 2022, Wang et al isolate five fatty acid compounds from Agaricus blazei, all of which showed inhibitory effects against the phytopathogenic bacterium, Lactobacillus michiganensis [25].…”

Section: Aliphatic Compoundsmentioning

confidence: 99%

Research Advances in Secondary Metabolites and Biological Activities of Mushroom Genus Fungi

2023

AJALS

View full text Add to dashboard Cite

Agaricus fungi are widely distributed in nature, their secondary metabolites with novel structures and significant biological activities, mainly including steroids, terpenoids, nitrogen compounds, sphingoids, aliphatic compounds and other structural types, some of them possessed anti-tumor, antibacterial, anti-neurolysin, anti-angiotensin-converting enzyme activities. The secondary metabolites and their biological activities of Agaricus fungi in recent 20 ye ars were reviewed to provide theoretical basis for further research and new drug development.

show abstract

Improving infant food safety by avoiding hazards of chemical mixture effects using novel integrated methods based on bioassays and analytical chemistry

et al. 2022

View full text Add to dashboard Cite

Effect-directed analysis and chemical identification of agonists of peroxisome proliferator-activated receptors in white button mushroom

Abstract: Linoleic acid is the main PPAR ligand in white button mushrooms and may be involved in their anti-obesogenic effect.

Cited by 4 publications

References 52 publications

Biochar Modulates Wheat Root Metabolome and Rhizosphere Microbiome in a Feedstock-dependent Manner

Biochar Modulates Wheat Root Metabolome and Rhizosphere Microbiome in a Feedstock-dependent Manner

Research Advances in Secondary Metabolites and Biological Activities of Mushroom Genus Fungi

Improving infant food safety by avoiding hazards of chemical mixture effects using novel integrated methods based on bioassays and analytical chemistry

Contact Info

Product

Resources

About