Effect directed analysis of riverine sediments—The usefulness of Potamopyrgus antipodarum for in vivo effect confirmation of endocrine disruption

Schmitt, Claudia Job; Streck, Georg; Lamoree, M.H.; Leonards, P.E.G.; Brack, Werner; Deckere, Eric de

doi:10.1016/j.aquatox.2010.10.002

Cited by 27 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nonylphenol has been reported before as a binder to TTR from several species (Table S1 ), but these studies did not report which form of nonylphenol was tested. Earlier analysis of exactly the same sample reported a triclosan concentration of 26 ng/g d.w. sediment and a nonylphenol concentration of 210 ng/g d.w. [ 32 ]. Based on their REP values and concentrations, these two compounds could explain less than 1 % of the observed TTR-binding potency in the unfractionated extract.…”

Section: Resultsmentioning

confidence: 99%

Tracing thyroid hormone-disrupting compounds: database compilation and structure-activity evaluation for an effect-directed analysis of sediment

et al. 2015

View full text Add to dashboard Cite

A variety of anthropogenic compounds has been found to be capable of disrupting the endocrine systems of organisms, in laboratory studies as well as in wildlife. The most widely described endpoint is estrogenicity, but other hormonal disturbances, e.g., thyroid hormone disruption, are gaining more and more attention. Here, we present a review and chemical characterization, using principal component analysis, of organic compounds that have been tested for their capacity to bind competitively to the thyroid hormone transport protein transthyretin (TTR). The database contains 250 individual compounds and technical mixtures, of which 144 compounds are defined as TTR binders. Almost one third of these compounds (n = 52) were even more potent than the natural hormone thyroxine (T4). The database was used as a tool to assist in the identification of thyroid hormone-disrupting compounds (THDCs) in an effect-directed analysis (EDA) study of a sediment sample. Two compounds could be confirmed to contribute to the detected TTR-binding potency in the sediment sample, i.e., triclosan and nonylphenol technical mixture. They constituted less than 1 % of the TTR-binding potency of the unfractionated extract. The low rate of explained activity may be attributed to the challenges related to identification of unknown contaminants in combination with the limited knowledge about THDCs in general. This study demonstrates the need for databases containing compound-specific toxicological properties. In the framework of EDA, such a database could be used to assist in the identification and confirmation of causative compounds focusing on thyroid hormone disruption.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-015-8736-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Tracing thyroid hormone-disrupting compounds: database compilation and structure-activity evaluation for an effect-directed analysis of sediment

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This type of data is often best achieved with in vitro assays, for example, cellular toxicity tests with endpoints including mutagenicity, genotoxicity, and endocrine disruption (e.g., Thomas et al and Weiss et al ) (Figure ). However, small‐scale whole‐organism toxicity testing (i.e., in vivo) has also been applied in some instances in EDA . As noted earlier, the use of organic solvent extracts in the in vitro assays precludes the EDA from addressing the bioavailability of toxicants (except, possibly, in the case of EDA conducted on exposed biota).…”

Section: Basic Approaches Methods and Applications In Tie And Edamentioning

confidence: 99%

Effects‐directed analysis (EDA) and toxicity identification evaluation (TIE): Complementary but different approaches for diagnosing causes of environmental toxicity

Burgess

Brack

et al. 2013

Enviro Toxic and Chemistry

Self Cite

112

View full text Add to dashboard Cite

Currently, 2 approaches are available for performing environmental diagnostics on samples like municipal and industrial effluents, interstitial waters, and whole sediments to identify anthropogenic contaminants causing toxicological effects. One approach is toxicity identification evaluation (TIE), which was developed primarily in North America to determine active toxicants to whole-organism endpoints. The second approach is effects-directed analysis (EDA), which has origins in both Europe and North America. Unlike TIE, EDA uses primarily in vitro endpoints with an emphasis on organic contaminants as the cause of observed toxicity. The 2 approaches have fundamental differences that make them distinct techniques. In EDA, the sophisticated and elegant fractionation and chemical analyses performed to identify the causes of toxicity with a high degree of specificity often compromise contaminant bioavailability. In contrast, in TIE, toxicant bioavailability is maintained and is considered critical to accurately identifying the causes of environmental toxicity. However, maintaining contaminant bioavailability comes with the cost of limiting, at least until recently, the use of the types of sophisticated fractionation and elegant chemical analyses that have resulted in the high specificity of toxicant diagnosis performed in EDA. The present study provides an overview of each approach and highlights areas where the 2 approaches can complement one another and lead to the improvement of both.

show abstract

“…Another EDA study by Schmitt et al (2011) on the sediment from Een, using a high-resolution three coupled normal-phase HPLC fractionation led to very promising results. Some fractions stimulated the reproduction of the snails just as it was observed for the native sediment samples and some candidate compounds like phthalates and bisphenol-A could be identified as probable key pollutants.…”

Section: Resultsmentioning

confidence: 92%

Sediment contact test with Potamopyrgus antipodarum in effect-directed analyses—challenges and opportunities

Schmitt

Vogt

Machala

et al. 2011

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

show abstract

Effect directed analysis of riverine sediments—The usefulness of Potamopyrgus antipodarum for in vivo effect confirmation of endocrine disruption

Cited by 27 publications

References 34 publications

Tracing thyroid hormone-disrupting compounds: database compilation and structure-activity evaluation for an effect-directed analysis of sediment

Tracing thyroid hormone-disrupting compounds: database compilation and structure-activity evaluation for an effect-directed analysis of sediment

Effects‐directed analysis (EDA) and toxicity identification evaluation (TIE): Complementary but different approaches for diagnosing causes of environmental toxicity

Sediment contact test with Potamopyrgus antipodarum in effect-directed analyses—challenges and opportunities

Contact Info

Product

Resources

About