Effect of 1,2-Dimethylhydrazine on Spleen Immunomorpholog

Abstract

The article presents the main morphological and immunohistochemical changes of the spleen in rats in 1 and 4 months after the course injection of 1,2-dimethylhydrazine. The increase in the number of CD68 + -cells in the red pulp and S100+cells in the germinal centers of lymphoid nodules was registered 1 month after injection in the spleen. The number of CD79α + and CD45RO + cells in white pulp structures, on the other hand, decreases. The area of lymphoid nodules and their germinal centers, the width of the marginal zone and periarterial lymphoid couplings are reduced. The total number of secondary lymphoid nodules increases. Four months after the end of the course of carcinogen injections, there is a significant increase in the number of CD68 + -cells in the red spleen pulp. Changes in the number of CD68 + and S100 + cells in germination centers are unreliable. The number of CD79α + and CD45RO + cells is significantly reduced. Changes in the morphological phenotype of the spleen at this time consist in a more pronounced reduction in the area of the lymphoid nodules with their germinal centers, the width of the periarterial lymphoid couplings and the marginal zone, as well as an increase in the number of secondary lymphoid nodules.