Effect of 1-methylcyclopropene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations

Sisler, Edward C.; Dupille, Éve; Serek, Margrethe

doi:10.1007/978-94-009-0131-5_17

Cited by 94 publications

(83 citation statements)

References 21 publications

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“…Figure 1D shows that seedlings germinated in the presence of both 1-MCP and ethylene displayed increases in root penetration with increasing ethylene concentration, indicating that ethylene could counteract the 1-MCP-induced inhibition of root penetration. This agrees with previous reports that exogenously applied ethylene can partially restore the 1-MCP-induced inhibition of ethylene receptors in some plant species (Sisler et al, 1996;Binder and Bleecker, 2003;Feng et al, 2004;Sisler, 2006). Taken together, these observations strongly support a role of ethylene in regulating root penetration.…”

Section: Inhibition Of Ethylene Signaling Impairs Root Penetration Insupporting

confidence: 92%

“…To study the role of ethylene in root growth in tomato, we germinated tomato seeds on Soilrite in the presence of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene receptors (Sisler et al, 1996;Sisler, 2006). Amazingly, in contrast to control seedlings, which showed normal penetration, the roots of the 1-MCP-treated seedlings failed to penetrate into the Soilrite and grew in the air, forming loops (Fig.…”

Section: Inhibition Of Ethylene Signaling Impairs Root Penetration Inmentioning

confidence: 99%

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Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

et al. 2011

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During seed germination, emerging roots display positive gravitropism and penetrate into the soil for nutrition and anchorage. Tomato (Solanum lycopersicum) seeds germinated in the presence of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, failed to insert roots into Soilrite and grew in the air, forming loops. Time-lapse video imaging showed that 1-MCPgrown root tips retained positive gravitropism and made contact with the surface of Soilrite but failed to penetrate into the Soilrite. Time-course studies revealed that the effect of 1-MCP was most prominent when seed imbibition and germination were carried out in the continual presence of 1-MCP. Conversely, 1-MCP was ineffective when applied postgermination after penetration of roots in the Soilrite. Furthermore, treatment with 1-MCP caused a reduction in DR5::b-glucuronidase auxinreporter activity and modified the expression of SlIAA3 and SlIAA9 transcripts, indicating interference with auxin signaling. The reduced ethylene perception mutant, Never-ripe, displayed decreased ability for root penetration, and the enhanced polar auxin transport mutant, polycotyledon, showed a nearly normal root penetration in the presence of 1-MCP, which could be reversed by application of auxin transport inhibitors. Our results indicate that during tomato seed germination, a coaction between ethylene and auxin is required for root penetration into the soil.

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Section: Inhibition Of Ethylene Signaling Impairs Root Penetration Insupporting

confidence: 92%

Section: Inhibition Of Ethylene Signaling Impairs Root Penetration Inmentioning

confidence: 99%

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

et al. 2011

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“…Ethylene gas (1 L/L) treatment also induced a similar level of root hairs in the rhd6 root, as did 5 M ACC (data not shown), and the effect of exogenous ethylene or ACC could be blocked completely by 1-methylcyclopropene (1-MCP), the competitive inhibitor of ethylene binding to the receptors. We chose 1-MCP as an antagonist of ethylene action because of its high specificity of action and lack of deleterious side effects (Sisler et al, 1996;Hall et al, 2000). At 1 L/L, 1-MCP almost completely abolished ACC-induced root hair formation and the expression of AtEXP7 and AtEXP18 in rhd6 (Figure 4).…”

Section: Exogenous Ethylene Is a Positive Effector For The Expressionmentioning

confidence: 99%

Regulation of Root Hair Initiation and Expansin Gene Expression in Arabidopsis[W]

2002

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The expression of two Arabidopsis expansin genes ( AtEXP7 and AtEXP18 ) is tightly linked to root hair initiation; thus, the regulation of these genes was studied to elucidate how developmental, hormonal, and environmental factors orchestrate root hair formation. Exogenous ethylene and auxin, as well as separation of the root from the medium, stimulated root hair formation and the expression of these expansin genes. The effects of exogenous auxin and root separation on root hair formation required the ethylene signaling pathway. By contrast, blocking the endogenous ethylene pathway, either by genetic mutations or by a chemical inhibitor, did not affect normal root hair formation and expansin gene expression. These results indicate that the normal developmental pathway for root hair formation (i.e., not induced by external stimuli) is independent of the ethylene pathway. Promoter analyses of the expansin genes show that the same promoter elements that determine cell specificity also determine inducibility by ethylene, auxin, and root separation. Our study suggests that two distinctive signaling pathways, one developmental and the other environmental/ hormonal, converge to modulate the initiation of the root hair and the expression of its specific expansin gene set.

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“…Fruit harvested at stage 0 that did not receive 1-MCP application presented ideal consumption conditions after five days of ripening (Figure 1). Even 1-MCP binding with the receptor in an irreversible way, fruit can reassume its ripening once new receptors are formed (SISLER et al, 1996). Probably, few molecules of receptor were formed or activated in fruit harvested at stage 0 at the application time, and, for that reason, 1-MCP could block a high percentage of receptor sites, prejudicing the receptor turnover and consecutively the ripening reassuming.…”

Section: Resultsmentioning

confidence: 99%

Influence of ripening stage on physical and chemical attributes of 'Golden' papaya fruit treated with 1-Methylcyclopropene

2006

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The 1-methylcyclopropene (1-MCP) has been used to extent postharvest life of various species. Generally, 1-MCP effectiveness decreases when applied in fruits at advanced ripening stages. The aim of this work was to verify the influence of ripening stages on some physical and chemical attributes of 'Golden' papaya treated with 1-MCP. Papayas were harvested at five ripening stages and treated with 100 nL L -1 of 1-MCP during 12 hours at 23ºC. Fruit skin color and pulp firmness were daily analyzed while soluble solids, titratable acidity and ascorbic acid were evaluated when fruit reached the consumption firmness (20 N). 1-MCP delayed firmness loss during fruit ripening of all ripening stages. The use of 1-MCP prejudices the softening of fruit harvested at stage 0 (totally green), which did not reach the consumption firmness. Although 1-MCP application at early stages was responsible for a significant increment in commercialization period, treated fruit stayed in the consumption condition for few days. When applied to a more advanced stage, 1-MCP not only increased the commercialization period, but also prolonged the period in that fruit stayed in consumption condition. 1-MCP also delayed the color change, but with a lower effect when compared to firmness retention. Ascorbic acid, titratable acidity and soluble solids were not influenced by 1-MCP application. The application of 1-MCP in fruit harvested at more advanced ripening stages could be an interesting practice since it does not prevent the ripening and still could prolong the period in which fruit stay in consumption condition. Key words:Carica papaya, postharvest, conservation. RESUMO INFLUÊNCIA DO ESTÁDIO DE AMADURECIMENTO EM ATRIBUTOS FÍSICOS E QUÍMICOS DO MAMÃO 'GOLDEN' TRATADO COM 1-METILCICLOPROPENOO 1-metilciclopropeno (1-MCP) tem sido usado para estender a vida pós-colheita de várias espécies. Geralmente, a eficiência do 1-MCP diminui quando aplicado em frutos em estádio de maturação avançado. O objetivo deste trabalho foi verificar a influência do estádio de maturação em alguns atributos físicos e químicos do mamão 'Golden' tratado com 1-MCP. Os mamões foram colhidos em cinco estádios de amadurecimento e tratados com 100 nL L -1 de 1-MCP durante 12 horas a 23 ºC. Os frutos foram diariamente avaliados quanto à cor da casca e firmeza da polpa, enquanto os sólidos solúveis, a acidez titulável e ácido ascórbico analisados quando os frutos atingiram a firmeza de consumo (20 N). O 1-MCP retardou a perda de firmeza durante o amadurecimento dos frutos em todos os estádios de amadurecimento. O uso do 1-MCP prejudicou o amolecimento de frutos colhidos no estádio 0 (totalmente verde), os quais não atingiram a firmeza de consumo. Embora a aplicação do 1-MCP em estádios mais precoces tenha sido responsável por um incremento significativo no período de comercialização, os frutos tratados Palavras chave: Carica papaya, conservação, pós-colheita. MATERIALS AND METHODS'Golden' papaya (Carica papaya L.) fruit were harvested in March 2004, from a commercial or...

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Effect of 1-methylcyclopropene and methylenecyclopropane on ethylene binding and ethylene action on cut carnations

Cited by 94 publications

References 21 publications

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

Tomato Root Penetration in Soil Requires a Coaction between Ethylene and Auxin Signaling

Regulation of Root Hair Initiation and Expansin Gene Expression in Arabidopsis[W]

Influence of ripening stage on physical and chemical attributes of 'Golden' papaya fruit treated with 1-Methylcyclopropene

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