Effect of 1-methylcyclopropene on the development of black mold disease and its potential effect on alternariol and alternariol monomethyl ether biosynthesis on tomatoes infected with Alternaria alternata

Estiarte, N.; Crespo-Sempere, A.; Marı́n, Sonia; Sanchís, V.; Ramos, Ana M. González

doi:10.1016/j.ijfoodmicro.2016.07.001

Cited by 20 publications

(17 citation statements)

References 31 publications

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“…Research on microbial VOCs fumigation has demonstrated successful antifungal effects on a large spectrum of pathogenic fungi and bacteria 3,33 . Despite these advances, plant diseases caused by B. cinerea and A. alternata remain a challenge 14,34 .…”

Section: Discussionmentioning

confidence: 99%

“…The present study investigated A. pullulans antifungal VOCs against two necrotrophic fungal pathogens; Botrytis cinerea and Alternaria alternata. The two pathogens infect a number of horticultural crops causing both pre and postharvest yield losses worldwide 13,14 . Black mould and grey mould are two plant diseases attributed to A. alternata and B. cinerea respectively 13,14 .…”

mentioning

confidence: 99%

“…The two pathogens infect a number of horticultural crops causing both pre and postharvest yield losses worldwide 13,14 . Black mould and grey mould are two plant diseases attributed to A. alternata and B. cinerea respectively 13,14 . In addition to yield losses, the production of deleterious off flavours leads to a loss of wine quality when wine is made with grey mould infected grapes 15 .…”

mentioning

confidence: 99%

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Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro

Don

Schmidtke

Gambetta

et al. 2020

Sci Rep

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Volatile organic compounds (VOCs) produced by Aureobasidium pullulans were investigated for antagonistic actions against Alternaria alternata and Botrytis cinerea. Conidia germination and colony growth of these two phytopathogens were suppressed by A. pullulans Vocs. A novel experimental setup was devised to directly extract VOCs using solid-phase microextraction-gas chromatographymass spectrometry (SPME-GC-MS) from antagonist-pathogen culture headspace. The proposed system is a robust method to quantify microbial VOCs using an internal standard. Multivariate curve resolutionalternating least squares deconvolution of SPME-GC-MS spectra identified fourteen A. pullulans Vocs. 3-Methyl-1-hexanol, acetone, 2-heptanone, ethyl butyrate, 3-methylbutyl acetate and 2-methylpropyl acetate were newly identified in A. pullulans headspace. Partial least squares discriminant analysis models with variable importance in projection and selectivity ratio identified four VOCs (ethanol, 2-methyl-1-propanol, 3-methyl-1-butanol and 2-phenylethanol), with high explanatory power for discrimination between A. pullulans and pathogen. The antifungal activity and synergistic interactions of the four VOCs were evaluated using a Box-Behnken design with response surface modelling. Ethanol and 2-phenylethanol are the key inhibitory A. pullulans VOCs against both B. cinerea and A. alternata. Our findings introduce a novel, robust, quantitative approach for microbial VOCs analyses and give insights into the potential use of A. pullulans Vocs to control B. cinerea and A. alternata.Microbial antagonists have been widely explored as more environmentally friendly disease management alternatives to reduce the excessive use of synthetic fungicides 1,2 . Among a wide array of mechanisms exploited by microbial antagonists, the production of volatile organic compounds (VOCs) with antimicrobial properties 3 has become popular in the recent research due to their low risk of toxic residues, biodegradability 4 and enormous spectrum of activity regardless of a physical contact of the commodity 5 .Aureobasidium pullulans is a yeast-like saprophytic fungus, naturally inhabiting plant and fruit surfaces 6 . This yeast is a well-known biocontrol agent against a range of pathogenic fungi 7,8 and strains of the organism have been formulated as commercial preparations for the practical management of fungal phytopathogens of a range of horticultural crops 9 . Production of antifungal VOCs has been recently identified as a potential mode of action for its biocontrol 10,11 . One of these studies has confirmed the identity of its four VOCs, 2-methyl-1-butanol, 3-methyl-1-butanol, 2-methyl-1-propanol and 2-phenylethanol 11 . These compounds were shown to suppress conidia germination and mycelium growth of Botrytis cinerea, Colletotrichum acutatum, Penicillium expansum, Penicillium digitatum and Penicillium italicum under both in vitro and in vivo conditions 11 . A similar study identified over 20 VOCs from A. pullulans isolated from grapevine 12 . However, none of these c...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro

Don

Schmidtke

Gambetta

et al. 2020

Sci Rep

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“…The LOQ was deemed to be the lowest concentration that could be quantified reliably. The Cq of the LOQ was determined using the following equation: Estiarte et al (2016). For mycotoxin quantification, working standard solutions were used to perform a ten-point calibration curve (1500, 1250, 1000, 750, 500, 250, 100, 50, 25 and 10 ng/mL).…”

Section: Dna Quantification From Viable Conidia Using the Pma-qpcr Tementioning

confidence: 99%

“…All solvents were HPLC grade and all chemicals were analytical grade. Method performance characteristics for AOH and AME are detailed in Estiarte et al (2016).…”

Section: Dna Quantification From Viable Conidia Using the Pma-qpcr Tementioning

confidence: 99%

Occurrence ofAlternaria mycotoxins and quantification of viableAlternaria spp. during the food processing of tomato products in Spain

Estiarte

Crespo-Sempere

Marı́n

et al. 2018

WMJ

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The occurrence of two Alternaria mycotoxins, alternariol (AOH) and alternariol monomethyl ether (AME) and the presence of conidia from Alternaria spp., were investigated throughout the food production chain of two businesses, one which uses organic fruit and the other non-organic. For this purpose, a propidium monoazide (PMA) treatment followed by a quantitative Real Time PCR (qPCR) was used to detect and quantify viable conidia exclusively. Results demonstrated that 68.4% of the total raw fruit analysed was contaminated with viable Alternaria spp. Regarding the mycotoxin occurrence, only a few samples were contaminated with AME, while 35% of raw tomatoes tested positive for AOH in the organic producer and 21% in the non-organic producer. AOH was present in samples analysed before heat treatment, while almost no mycotoxins were found in the final products of the organic producer. However, in the non-organic producer, 47% of the tomato concentrates were contaminated.

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Effects of an Active Cardboard Box Using Encapsulated Essential Oils on the Tomato Shelf Life

Buendía−Moreno

Ros−Chumillas

Navarro−Segura

et al. 2019

Food Bioprocess Technol

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Effect of 1-methylcyclopropene on the development of black mold disease and its potential effect on alternariol and alternariol monomethyl ether biosynthesis on tomatoes infected with Alternaria alternata

Cited by 20 publications

References 31 publications

Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro

Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro

Occurrence ofAlternaria mycotoxins and quantification of viableAlternaria spp. during the food processing of tomato products in Spain

Effects of an Active Cardboard Box Using Encapsulated Essential Oils on the Tomato Shelf Life

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