Effect of 1 mT sinusoidal electromagnetic fields on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stromal cells

Mazurek

et al. 2017

Ann Agric Environ Med.

Electromagnetic radiation (EMR) emitting from the natural environment, as well as from the use of industrial and everyday appliances, constantly influence the human body. The effect of this type of energy on living tissues may exert various effects on their functioning, although the mechanisms conditioning this phenomenon have not been fully explained. It may be expected that the interactions between electromagnetic radiation and the living organism would depend on the amount and parameters of the transmitted energy and type of tissue exposed. Electromagnetic waves exert an influence on human reproduction by affecting the male and female reproductive systems, the developing embryo, and subsequently, the foetus. Knowledge concerning this problem is still being expanded; however, all the conditionings of human reproduction still remain unknown. The study presents the current state of knowledge concerning the problem, based on the latest scientific reports.

Section: Mechanisms Of the Effect Of Emrsupporting

confidence: 85%

Effect of electromagnetic waves on human reproduction

Mazurek

et al. 2017

Ann Agric Environ Med.

“…For instance, in vitro exposure to pulsed electromagnetic fields (PEMF) at extremely low frequencies (ELF) of 15 Hz or 50 Hz, has been shown to significantly increase cell proliferation by 20% to 60% over controls, in human [48] and rat BMSC [49]. Also, 50 Hz sine wave fields of 0.5 to 1 mT have been reported to promote proliferation of rodent BMSC [29,50]. Although regenerative therapies applying signal frequencies within the intermediate-to-radiofrequency spectrum have expanded in recent years, no experimental evidence has been obtained to date that electric or electromagnetic stimulation at such frequencies could promote stem cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Electric Stimulation at 448 kHz Promotes Proliferation of Human Mesenchymal Stem Cells

Hernández-Bule¹,

Paı́no²,

Trillo³

et al. 2014

Cell Physiol Biochem

102

108

Background/Aims: Capacitive-resistive electric transfer (CRET) is a non invasive electrothermal therapy that applies electric currents within the 400 kHz - 450 kHz frequency range to the treatment of musculoskeletal lesions. Evidence exists that electric currents and electric or magnetic fields can influence proliferative and/or differentiating processes involved in tissue regeneration. This work investigates proliferative responses potentially underlying CRET effects on tissue repair. Methods: XTT assay, flow cytometry, immunofluorescence and Western Blot analyses were conducted to asses viability, proliferation and differentiation of adipose-derived stem cells (ADSC) from healthy donors, after short, repeated (5 m On/4 h Off) in vitro stimulation with a 448-kHz electric signal currently used in CRET therapy, applied at a subthermal dose of 50 μA/mm²Results: The treatment induced PCNA and ERK1/2 upregulation, together with significant increases in the fractions of ADSC undergoing cycle phases S, G₂ and M, and enhanced cell proliferation rate. This proliferative effect did not compromise the multipotential ability of ADSC for subsequent adipogenic, chondrogenic or osteogenic differentiation. Conclusions: These data identify cellular and molecular phenomena potentially underlying the response to CRET and indicate that CRET-induced lesion repair could be mediated by stimulation of the proliferation of stem cells present in the injured tissues.

Molecular and Cellular Endocrinology

“…One of the reasons for the uncertain efficacy is the nonlinear window effects of PEMF parameters such as frequencies, intensities, duty ratio and the duration of exposure (Liu et al, 2013;Markov, 2005Markov, , 2007Sun et al, 2014;Zhou et al, 2014). The stable effective therapy can be obtained only by using optimal parameters which produce window effects.…”

Section: Introductionmentioning

confidence: 97%

Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia

Yan

Zhou

et al. 2015

Although pulsed electromagnetic fields (PEMFs) have been approved as a therapy for osteoporosis, action mechanisms and optimal parameters are elusive. To determine the optimal intensity, exposure effects of 50 Hz PEMFs of 0.6-3.6 mT (0.6 interval at 90 min/day) were investigated on proliferation and osteogenic differentiation of cultured calvarial osteoblasts. All intensity groups stimulated proliferation significantly with the highest effect at 0.6 mT. The 0.6 mT group also obtained the optimal osteogenic effect as demonstrated by the highest ALP activity, ALP(+) CFU-f colony formation, nodule mineralization, and expression of COL-1 and BMP-2. To verify our hypothesis that the primary cilia are the cellular sensors for PEMFs, osteoblasts were also transfected with IFT88 siRNA or scrambled control, and osteogenesis-promoting effects of 0.6 mT PEMFs were found abrogated when primary cilia were inhibited by IFT88 siRNA. Thus primary cilia of osteoblasts play an indispensable role in mediating PEMF osteogenic effect in vitro.