Effect of ?1-protease inhibitor (?1-antitrypsin on the intensity of transformation of phytohemagglutinin-stimulated lymphocytes

Baranova, F. S.; Berman, Alexandra; Zaretskaya, Yu. M.

doi:10.1007/bf00835498

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…In accordance with the proposed method, a negative slope of the regression line (r = −0.859; P < 0.01) was obtained in our present experiment, indicating a cytostatic effect of the ATI. The studies concerning the effect of alpha 1-antitrypsin (alpha trypsin inhibitor /1-AT) on human lymphocyte proliferation demonstrated that in vitro 1-AT could inhibit PHAinduced lymphocyte transformation (Bata et al 1977;Baranova et al 1980). The investigations conducted by Breit et al (1983) also reported suppression of the PHA response by purified 1-AT.…”

Section: Discussionmentioning

confidence: 90%

Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation

Błaszkowska

Bratkowska

Łopaczyńska

et al. 2009

J Genet

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The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 microg per ml of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50 and 100 microg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions.

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Section: Discussionmentioning

confidence: 90%

Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation

Błaszkowska

Bratkowska

Łopaczyńska

et al. 2009

J Genet

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“…While UTM-proteins have not been shown to exhibit protease inhibitory activity [28], the proteins do share with otl-antitrypsin the ability to bind IgA [47,48]. Additionally, al-antitrypsin can inhibit proliferation of lymphocytes at high concentrations similar to those at which UTM-proteins are effective [49,50]. Moreover, as for UTMproteins, a-i antitrypsin inhibited PHA and Con A-stimulated cells while not affecting cells stimulated with PWM [50].…”

Section: Discussionmentioning

confidence: 98%

Identification of the Predominant Proteins in Uterine Fluids of Unilaterally Pregnant Ewes that Inhibit Lymphocyte Proliferation1

Skopets

Hansen

1993

Biology of Reproduction

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Uterine fluid from unilaterally pregnant ewes contains activity inhibitory to lymphocyte proliferation. The molecules responsible for this activity may thereby regulate uterine immune responses during pregnancy. The purpose of the experiment described here was to identify the major protein in uterine fluid responsible for this activity. When uterine fluid was fractionated by a combination of cation exchange chromatography, gel filtration, and lectin affinity chromatography, the majority of the lymphocyte activity co-migrated with a pair of proteins previously identified as related, serpin-like glycoproteins. Together, this pair of proteins, called the uterine milk proteins (UTM-proteins), are the predominant endometrial secretory protein produced under the influence of progesterone. Preparations of uterine protein highly enriched for the UTM-proteins inhibited lymphocyte proliferation induced by phytohemagglutinin, concanavalin A, and mixed lymphocyte reactions but did not inhibit proliferation induced by pokeweed mitogen. In some experiments, UTM-proteins also reduced viability of cultured lymphocytes. Another previously described lymphocyte-inhibitory factor, megasuppressin, was also observed. Megasuppressin, which eluted at an apparent molecular weight of greater than 4 x 10(6) even after treatment with urea, guanidine-HCl, and beta-mercaptoethanol, was a more potent inhibitor of lymphocyte proliferation than UTM-proteins. Megasuppressin is not very abundant, however, and probably is responsible for only a small fraction of the lymphocyte inhibitory activity in uterine fluid. The majority of lymphocyte-inhibitory activity is caused by the UTM-proteins or by a molecule that co-purifies in trace amounts with UTM-proteins.

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Interaction between α1 antitrypsin and lymphocyte surface proteases: immunoregulatory effects

Bata

Revillard

1981

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Alpha 1 antitrypsin (alpha 1-AT) is the major plasma protease inhibitor. Radioiodinated alpha 1-AT binds to human lymphocytes. The binding is fast and reversible, and the cells can be saturated with a maximum of approximately 1.2 x 10(6) molecules of alpha 1-AT per lymphocyte. The receptor for alpha 1-AT is a surface-associated protease. Addition of alpha 1-AT completely inhibits cell surface proteolytic activity. Furthermore alpha 1-AT decreases 3H-thymidine incorporation into lymphocytes stimulated by B or T cell mitogens or by allogeneic cells. Since alpha 1-AT was shown to be produced by activated monocytes and to bind to lymphocytes, it is likely to represent a mediator of monocyte-lymphocyte interactions.

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Effect of ?1-protease inhibitor (?1-antitrypsin on the intensity of transformation of phytohemagglutinin-stimulated lymphocytes

Cited by 4 publications

References 8 publications

Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation

Cytogenetic study of Ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation

Identification of the Predominant Proteins in Uterine Fluids of Unilaterally Pregnant Ewes that Inhibit Lymphocyte Proliferation1

Interaction between α1 antitrypsin and lymphocyte surface proteases: immunoregulatory effects

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