Effect of 12-O-tetradecanoylphorbol-13-acetate-induced psoriasis-like skin lesions on systemic inflammation and atherosclerosis in hypercholesterolaemic apolipoprotein E deficient mice

Madsen, Marie; Hansen, Peter Riis; Nielsen, Lars Bo; Hartvigsen, Karsten; Pedersen, Anders Elm; Christensen, Jan Pravsgaard; Aarup, Annemarie; Pedersen, T.

doi:10.1186/s12895-016-0046-1

Cited by 22 publications

(22 citation statements)

References 37 publications

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“…ApoE À/À mice cutaneous lipids were excessive accumulation in which pathological changes of psoriasis corneum changed as dyslipideamia [13,14]. Recently, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ApoE À/À mice were reported, which might evoke systemic immune-inflammatory effects and question the role of psoriasis induced inflammation in the pathogenesis of atherosclerosis in psoriatic patients [15]. Based on the critical role of IL-23/ Th17 axis in psoriasis, topical application of imiquimod (IMQ), a TLR7/8 ligand and potent immune activator, could induce closely resembling human plaque-type psoriasis [16].…”

Section: Introductionmentioning

confidence: 99%

Imiquimod induced ApoE-deficient mice might be a composite animal model for the study of psoriasis and dyslipideamia comorbidity

Xie

Zhang

Lin

et al. 2017

Journal of Dermatological Science

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Section: Introductionmentioning

confidence: 99%

Imiquimod induced ApoE-deficient mice might be a composite animal model for the study of psoriasis and dyslipideamia comorbidity

Xie

Zhang

Lin

et al. 2017

Journal of Dermatological Science

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“…[ 32 ] In this study, “white” skin patches at similar position in the back skin were used for analysis as different hair cycles can influence the skin response to compounds and even the development of inflammation. [ 25,33 ] The SC in these “white” back skin patches of hairy mice has fewer number of corneocyte layers as compared to ear skin. The back skin of nude mice with a larger interfollicular area displays a thicker SC with more corneocyte layers, [ 34 ] suggesting that the reduced number of corneocyte layers in hairy mice is a direct effect of the presence of dense hair follicles and/or fur.…”

Section: Discussionmentioning

confidence: 99%

“…APOE −/− mice and other hypercholesterolaemic mouse models have recently been used to study the relation between psoriasis and the comorbidities dyslipidemia and atherosclerosis. [ 23‐25 ] Differential effects on the aggravation of dyslipidemia in APOE −/− mice have been described in response to the induction of psoriasis‐like skin inflammation by topical application of compounds on ear vs back skin, with back skin being more effective. [ 6,26 ] As lipids play a major role in the skin barrier, it is crucial to know whether there are differences in lipid barrier in ear vs back skin of APOE −/− mice.…”

Section: Introductionmentioning

confidence: 92%

Barrier lipid composition and response to plasma lipids: A direct comparison of mouse dorsal back and ear skin

Cardoso

Absalah

Eck

et al. 2020

Experimental Dermatology

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Experimental Dermatology. 2020;29:548-555. wileyonlinelibrary.com/journal/exd | INTRODUC TI ONOur understanding of skin morphology and lipid composition has benefitted greatly from the use of in vivo animal models. [1][2][3] In particular, mouse models have been proven a valuable tool in skin research as it offers the possibility to genetically manipulate these animals to study the role of specific skin components (eg enzymes, proteins, receptors) and to generate in vivo diseased skin models. [1,2] In research, the back skin and the ear skin of mice are commonly used sites. However, previous studies reported differential effects/ AbstractThe skin of the ear and the back are frequently selected sites in skin research using mouse models. However, distinct responses to treatment have been described between these two sites in several studies. Despite the crucial role of the stratum corneum (SC) in the skin barrier function of both dorsal back and ear skin, it remains unclear whether differences in lipid composition might underlie altered responses.Here, we compared the skin morphology and the barrier lipid composition of the ear with the back skin of wild-type mice. The ear contained more corneocyte layers in the SC and its barrier lipid composition was enriched with sphingosine ceramide subclasses, especially the short ones with a total chain length of 33-34 carbons. The free fatty acid (FFA) profile in the ear skin shifted towards shorter chains, significantly reducing the mean chain length to 23.3 vs 24.7 carbons in the back skin. In line, FFA species in the ear displayed a twofold increase in unsaturation index (P < .001). Gene expression in the ear skin revealed low expression of genes involved in lipid synthesis and uptake, indicating a reduced metabolic activity. Finally, the effects of hypercholesterolaemia on SC FFA composition was compared in ear and back skin of apolipoprotein E knockout (APOE −/− ) mice. Interestingly, the FFA profile in APOE −/− ear skin was minimally affected, while the FFA composition in the back skin was markedly changed in response to hypercholesterolaemia. In conclusion, ear and back skin have distinct barrier lipids and respond differently to elevated plasma cholesterol. K E Y W O R D S apolipoprotein E knockout mice, ceramides, fatty acids, wild-type mice | 549 MARTINS CARDOSO eT Al.phenotypes on ear skin vs the back skin regarding, among others, drug treatment (eg imiquimod-induced psoriasis, allergic contact dermatitis), melanocyte function and tissue regeneration. [4][5][6] The back skin comprises a relatively large area for performing experiments, but in hairy mice it contains a high density of hair follicles (fur) that can complicate the interpretation of the results. [5] In most studies, the fur is shaved to allow skin treatment and analysis. At the same time, the hair follicles may offer an alternative pathway for compound permeation. [7,8] In contrast, the ear represents an easily accessible, but small, skin area with a low density of hair follicles and centrally supported...

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“…An anti-inflammatory activity study of the SBKT was performed using the λ-Carrageenan-stimulated Wistar rat inflammation model and the TPA-stimulated CD-1 mice psoriasis-like model. The TPA-stimulated CD-1 mice psoriasis-like model is well-established for studying the disease-modulating efficacy of test compounds (Madsen et al, 2016; Ma et al, 2018; Yang et al, 2018). Treatment of the Carrageenan-stimulated Wistar rats with a human equivalent dose of SBKT showed a significant decrease in the drug-induced paw volume increase and edema in the rats.…”

Section: Discussionmentioning

confidence: 99%

Cytokines Driven Anti-Inflammatory and Anti-Psoriasis Like Efficacies of Nutraceutical Sea Buckthorn (Hippophae rhamnoides) Oil

et al. 2019

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Psoriasis is a chronic inflammatory skin disease characterized by circumscribed, red, thickened plaques with overlying silvery white scales. It is associated with the release of pro-inflammatory mediators that lead to the development of edema and distress. Here we show the anti-inflammatory and anti-psoriatic efficacies of a neutraceutical sea buckthorn oil (SBKT) derived from the fruit pulp of Hippophae rhamnoides. Chemical analysis of the SBKT showed the presence of 16 major saturated, mono-, and polyunsaturated fatty acids components, imparting significant nutritional values. Efficacy of the SBKT in modulating psoriasis and associated inflammation was first tested in vitro using human monocytic (THP-1) cells. SBKT induced cytotoxicity at a dose of ≥25 µl/ml. Treatment of the lipopolysaccharide-stimulated THP-1 cells with SBKT subdued the enhanced release of intracellular reactive nitrogen species and expression of NF-κB protein, in a concentration-dependent manner. This was accompanied by a reduction in the release of downstream pro-inflammatory cytokines: Interleukin-1ß and interleukin-6. Tumor necrosis factor-α released in the stimulated THP-1 cells were also inhibited by SBKT dose of 5 µl/ml. In vivo oral and topical treatment with SBKT in the Carrageenan-stimulated paw edema model, showed a significant decrease in paw volume and edema. In the 12-O tetradecanoyl phorbol 13-acetate (TPA) stimulated CD-1 mice psoriasis-like model, concurrent oral and tropical SBKT treatments substantially reduced ear edema and ear biopsy weights. Histopathologically, significant reduction in ear epidermal thickness and skin lesion scores was observed in the SBKT-treated animals. In conclusion, SBKT showed anti-inflammatory and anti-psoriasis-like efficacies in healing chemical-induced inflammation and psoriasis. The possible mode of action of SBKT was found through inhibition of reactive nitrogen species, and downregulation of NF-κB protein and pro-inflammatory cytokines. Thus, the present data suggest that Sea buckthorn oil can be used as an anti-inflammatory and anti-psoriatic nutraceutical.

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Effect of 12-O-tetradecanoylphorbol-13-acetate-induced psoriasis-like skin lesions on systemic inflammation and atherosclerosis in hypercholesterolaemic apolipoprotein E deficient mice

Cited by 22 publications

References 37 publications

Imiquimod induced ApoE-deficient mice might be a composite animal model for the study of psoriasis and dyslipideamia comorbidity

Imiquimod induced ApoE-deficient mice might be a composite animal model for the study of psoriasis and dyslipideamia comorbidity

Barrier lipid composition and response to plasma lipids: A direct comparison of mouse dorsal back and ear skin

Cytokines Driven Anti-Inflammatory and Anti-Psoriasis Like Efficacies of Nutraceutical Sea Buckthorn (Hippophae rhamnoides) Oil

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