Effect of 17 a-Methyl Testosterone on Haematology and Histology of Liver and Heart of Oreochromis andersonii (Castelnau, 1861)

As, Kefi; Kang'ombe, Jeremiah

doi:10.4172/2155-9910.1000130

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…Groups 3 and 4 showed normal central vessels that were not congested, on the other hand, group 5 showed mildly congested central vessels, the sinusoids appeared normal and not infiltrated by inflammatory cells. This finding agrees with a similar study by Khater (1998) in studying the effect of different doses (15, 30, 60, 90 mg) of 17α-methyltestosterone on the liver for 28 days and indicated that the hepatic parenchyma had diffused vacuolar degeneration while the central veins and hepatic sinusoids were congested, on the contrary, Deborah, (1990) and Kefi et al (2013) found that there was no deviation from the normal morphology in livers. However, in this study, there appeared to be differences in the hepatocytes, abundant glycogen vacuolation in groups 2 and 4.…”

Section: Discussionsupporting

confidence: 92%

“…There have been reports that hormones excreted by treated fish from undigested feeds can build up in a closed water system; accumulate in water residue and aquatic biota (Dauda et al, 2014). Kefi et al (2013) in their trial with tilapia fish, observed an induced sex inversion in waters not directly applied with methyltestosterone, although is not well understood; the effect on water pollution.…”

Section: Introductionmentioning

confidence: 99%

“…In recent times, the use of MT in the seed production of Oreochromis species has increased despite inadequate information about the impact of the androgen on fish physiology; though, its long-term impacts on the pathology of livers, gills and muscle remain unclear (Kefi et al, 2013). Curtis et al (1991) established the fact that metabolizes of plasma testosterone are quickly excreted from the body.…”

Section: Introductionmentioning

confidence: 99%

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Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Orose¹,

Wokeh²,

Odioko³

2021

EgeJFAS

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This study was conducted to evaluate the histological changes of 17αmethyl-testosterone (MT), wet testes of bull (Bos indicus), boar (Sus domesticus), bull/mud catfish testes (B. indicus/C. gariepinus) and pawpaw (Carica papaya) seed powder-based diet on the Nile tilapia fry in an indoor experimental pond. Histological changes and efficacies in producing monosex males were determined after four months. Fifty-five fry were assigned randomly to three experimental ponds in triplicates. Five experimental feeds composed of soya-bean, fish meal, wheat bran, cassava flour were formulated by adding 0.06g of 17α- methyl-testosterone, 17.47g of bull wet testes, 25.59g of boar wet testes, 20.48g of bull/catfish and 20g of pawpaw seed powder per kg of feed to group 1, 2, 3, 4, 5 respectively. Obtained results from the morphological examination revealed a significant difference (p < 0.05) in phenotypic males of the Nile tilapia fry fed with the experimental diets. The highest indistinct percentage was recorded in the 17αmethyl-testosterone-treated diet while the lowest was recorded in diets 3 and 4 with no significant differences. All the groups showed histopathological differences in the livers, gills, and muscles. However, the liver histology of groups 1 and 5 showed central vessels that are mildly congested with abundant glycogen vacuolation and somewhat fat infiltration, while the histology of the muscles of group 5 showed pathological lesions including moderate to severe necrosis. These alterations can be considered an indication of the performance and health of fish in monosex culture medium indicating effects of plant extract and MT.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Orose¹,

Wokeh²,

Odioko³

2021

EgeJFAS

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“…Nonetheless, hematological indicators of the fish's nutritional condition and general health have been said to be reliable (Katalay & Parlak, 2004) [31] , as they indicate the nutritional status and overall health of the fish (Akinrotimi et al, 2012) [32] . The fall in Ht and Hb of the fish after MT delivery may indicate that their condition has worsened as a result of the androgen therapy (AS & J, 2013) [33] . According to Joshi et al, 2002 [34] , fish may be less able to increase their activity to fulfil sporadic needs if their haemoglobin levels are lower.…”

Section: Discussionmentioning

confidence: 99%

Effect of synthetic hormone, 17α-ethinylestradiol on hematological and biochemical parameters in fish Channa punctatus (Bloch.1793)

Tarique¹,

Khatoon²,

Trivedi³

et al. 2023

Int. J. Fish. Aquat. Stud.

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Environmental Protection includes programs that are aimed to reducing risks to the environment from hazardous materials and chemical wastes. Nowadays, 17α-ethinylestradiol, a synthetic estrogen, has become one of the most commonly used active ingredients for oral contraception, interfere with the normal functioning of endocrine systems, thus affecting reproduction and development in wildlife. The fresh water food fish, Channa punctatus, was selected as the test animal to perform the desired experimental work. The physio-chemical parameters (Temperature, pH, DO, CO2, and Hardness) of test medium, hematological (WBC, RBC, Hb, PCV, MCV, MCH, and Platelet) and biochemical (Total protein, AST, ALT, ALP, Urea and Creatinine) parameters of the fish were analysed. Channa punctatus was exposed to three different concentrations of 17α-ethinylestradiol (5, 10 and 20 ng/L), along with a control group for 28 days and their samples were collected at an interval of 7, 14, 21 and 28 days. Result shows that hematological parameters-RBC, Hb, PCV, MCV, MCH and Platelet were found significantly decreased and WBC significantly increased and the biochemical parameters-AST, ALT, ALP and Urea were found significantly increased while, Total protein and Creatinine showed insignificant differences on exposure to 17α-ethinylestradiol. Therefore, the study stated that 17α-ethinylestradiol produce an adverse effect in hematological and biochemical parameter which might affect normal physiology and immunity of fish.

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“…Sex reversal % CLA50 96% CLA100 91% Plate 2 Pictures of some livers extracted under the study DISCUSSION Kefi et al,(2013) reported red liver coloration (and not brownish liver coloration) for Oreochromis andersonii treated with 0mg/kg , 40mg/kg and 60mg/kg in their short study on the effects of 17α-Methyltestosterone (MT) on haematology and histology of the liver.…”

Section: Treatmentmentioning

confidence: 91%

EFFECTS OF 17α-METHYLTESTOSTERONE (MT) ON THE LIVER OF MALE AND FEMALE Clarias gariepinus BROODSTOCKS AFTER WEANING OFF THE HORMONE-TREATED FEED

E.A.¹

2021

SCIRJ

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Clarias gariepinus fry numbering four hundred and fifty, were stocked in nine concrete tanks. Stocking density was fifty fry per 2 X 2 X 1 metre square tank. The duration for the experiment was three hundred and sixty-five (365) days. Feeding with the hormone ended at 365 days from which normal feeding without the use of the hormone commenced for another 365 days. Clarias gariepinus. fry were fed 0.2mm coppens feed mixed with 17α-Methyltestosterone (MT) at 10% of the fish' body weight from start of the experiment till they were one month old. Subsequently, fish were fed 0.8mm coppens feed from two months old till three months old. Feeding was administered at 10% of the fish' body weight. Clarias gariepinus fish were fed 2mm coppens at four months old. Feeding was also done at 10% of the fish' body weight. 6mm coppens feed was administered to fishes from five months old till twelve months at 5% of the fish' body weight. Thereafter, fish were fed 5% of the fish' body weight for another 365 days. Mean initial weights ranged from 0.053g to 0.065g. Mean final weights ranged from 6.7g to 8.8g. Liver weights for the CLA50 treatments ranged from 3.7g to 8.0g for the male Clarias gariepinus; while the female Clarias gariepinus also in the CLA50 treatment showed liver weights of 4.0g to 12.6g. The CLA100 treatments showed the following weights for the male Clarias gariepinus and the female Clarias gariepinus under the study -6.6g to 14.2g and 5.9g to 18.2g respectively. There were three treatments under study: CLA50, CLA100, CLA0. Treatments were triplicated.

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Effect of 17 a-Methyl Testosterone on Haematology and Histology of Liver and Heart of Oreochromis andersonii (Castelnau, 1861)

Cited by 3 publications

References 26 publications

Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Effect of synthetic hormone, 17α-ethinylestradiol on hematological and biochemical parameters in fish Channa punctatus (Bloch.1793)

EFFECTS OF 17α-METHYLTESTOSTERONE (MT) ON THE LIVER OF MALE AND FEMALE Clarias gariepinus BROODSTOCKS AFTER WEANING OFF THE HORMONE-TREATED FEED

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