Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

Abstract

The normal dose of 17α-methyltestosterone (MT) used in fish farming was 60 mg/L, and now the analysis of residual androgens was carried out in waste water obtained from the Beijing area, which could be detected in levels ranging from 4.1 to 7.0 ng/L. For the purpose of aquatic early warning, the present study clearly demonstrated that chronic exposure by higher concentration of MT than environmental relevant concentrations could trigger oxidative stress response to juvenile tilapia by modulating hepatic antioxidant enzyme activities and gene transcription. Some antioxidative parameters (T-GSH, GSH/GSSG and MDA) were significant decreased under 0.5 mg/L MT exposure at 7 and 14 days. Some antioxidant enzymes (SOD, CAT and GST) and transcriptional changes (sod and cat) were revealed significant decreases for MT treated groups at 7 days. Total antioxidant capacity was significant increased only in 5 mg/L MT exposure groups, but GR activities were not affected all through the whole exposure period. Almost all of the antioxidant enzymatic genes detected in the present study were showed significant increments for MT exposure both at 14 and 21 days, and the genotoxicity profile of antioxidant enzymatic genes were revealed dose-dependent manner. This study presented evidence that MT could result in oxidative stress response in the early stages of GIFT tilapia.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-1946-6) contains supplementary material, which is available to authorized users.

The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia,Oreochmomis niloticus(Linnaeus, 1758)

“…Contrary to our observation, Zheng et al. (2016) noticed reduced SOD and CAT activities in juvenile GIFT tilapia liver exposed to MT solution for different time periods. On the other hand, MT exposure was found not to alter CAT activity in zebrafish embryo (Rivero‐Wendt et al., 2016).…”

Dietary supplementation with probiotics regulates gut microbiota structure and function in Nile tilapia exposed to aluminum

“…In 2015, its production accounted for more than 10% of all farmed fish worldwide (FAO, 2015). Moreover, tilapia are recognized as a good biological model because they are easy to handle, culture, and maintain in the laboratory (Korkmaz et al, 2009), and because they display excellent stress sensitivity (Zheng et al, 2016). The aim of the study was to explore whether the regulation of gut microbiota is an aluminum toxicity alleviation mechanism exerted by probiotics in tilapia.…”

Fish diet and male reproductive hormones in albino rats

“…As for aquaculture, rearing of fish is practiced in ponds which are fertilized with manure fertilizers (chicken manure and cattle manure) and chemical fertilizers (urea and super phosphate) to allow production up to 2000 to 3000 kg/hectare/crop. In addition, the fish larvae are fed with feeds that are incorporated with the synthetic steroid 17α-methyl testosterone (17 α-MT) which is a male-specific hormone commonly used to induce sex reversal in farmed fish at a concentration of 60 mg/kg of feed (Zheng et al, 2016). 17α-MT treatment results in a final size of fish 10.7% larger and more uniform size than mixed sex fish (Ahmad, Shalaby, Khattab, & Abdel-Tawwab, 2002).…”