Effect of 17β-estradiol on immunoglobulin secretion by human tonsillar lymphocytes In vitro

Evagelatou, Marianthi; Farrant, J.

doi:10.1016/0960-0760(94)90142-2

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…In addition, in vitro studies have shown the direct effect of oestrogens on the activation, proliferation and differentiation of cells of the immune system. Previous observations from our group have shown that OE2 has significant effects on immunoglobulin production and DNA synthesis by tonsillar lymphocytes in vitro (Evagelatou & Farrant 1994). Preliminary data from our studies have shown the presence of ERs in tonsillar cells, mainly T cells.…”

Section: Discussionsupporting

confidence: 56%

“…The mononuclear cell preparation obtained from the interface consisted mainly of CD19+ (B) cells, CD3 (T) cells and a small proportion of CD14 (monocytes). In some experiments, mononuclear cells were rosetted overnight with neuraminidasetreated sheep erythrocytes (n-SRBC) to separate T cells from the preparation (Evagelatou & Farrant 1994). The T cells were recovered by lysing the treated sheep erythrocytes with ammonium chloride lysis fluid.…”

Section: Cell Preparationsmentioning

confidence: 99%

“…Human tonsils were obtained after routine oper¬ ations for chronic tonsillitis and used as sources of lymphocytes (Evagelatou & Farrant 1994). Tonsil material was used with the approval of the Ethical Committee of the MRC Clinical Research Centre and Northwick Park Hospital.…”

Section: Cell Preparationsmentioning

confidence: 99%

“…We have previously shown that OE2 has significant effects on immunoglobulin production by resting or stimulated tonsillar B and T lymphocytes and on DNA synthesis by resting or stimulated tonsillar T cells in vitro (Evagelatou & Farrant 1994 …”

Section: Introductionmentioning

confidence: 99%

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Effect of oestradiol-17β on the expression of oestrogen receptor mRNA in human tonsillar cells

Evagelatou

Farrant²

1995

Journal of Molecular Endocrinology

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We have investigated the expression of oestrogen receptor (ER) mRNA in Ficoll-separated tonsillar cells and the changes that occur with the addition of oestradiol (OE2) both in the presence and the absence of the T cell mitogen phytohaemagglutinin (PHA). The amounts of ER mRNA and beta-actin mRNA in the samples were determined by slot blotting and hybridization and quantified by densitometry. The levels of ER mRNA were normalized against the beta-actin mRNA content. In the presence of OE2 (7 x 10(-8) M) after a 10-h culture there was a significant decrease (P < 0.05) to about 66% of the control (0-h culture) ER mRNA levels. Stimulating the cultures with PHA (1 microgram/ml), without the presence of OE2, had no effect on the expression of ER mRNA. However, when OE2 was present in a 10-h culture of PHA-stimulated cells, the ER mRNA level was significantly decreased (P < 0.05) to about 60% of control levels. In 24-h cultures, the presence of OE2 and/or PHA had no effect. When separated T cell preparations from the tonsils were used, no significant effects of OE2 were seen in either the 10-h or 24-h cultures. In conclusion, OE2 downregulates the ER mRNA content in a tonsillar mononuclear cell system in vitro as it does in many primary oestrogen target cells.

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Section: Discussionsupporting

confidence: 56%

Section: Cell Preparationsmentioning

confidence: 99%

Section: Cell Preparationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of oestradiol-17β on the expression of oestrogen receptor mRNA in human tonsillar cells

Evagelatou

Farrant²

1995

Journal of Molecular Endocrinology

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“…Estrogen has been shown to increase B-cell activity and antibody production (97) and also to promote IL-2-driven Bcell production of IgM and IgG in spleen cells while downregulating IgG production by normal blood B cells (38). The downregulatory effects of estrogen on T-cell development in the thymus have been discussed above, but in addition this hormone has also been shown to deplete T cells, particularly CD8 ϩ T cells, from a number of tissues, including the genital mucous membranes and lymphoid tissues of the gut (23).…”

Section: Lymphocytesmentioning

confidence: 99%

Sex-Associated Hormones and Immunity to Protozoan Parasites

2001

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Numerous epidemiological and clinical studies have noted differences in the incidence and severity of parasitic diseases between males and females. Although in some instances this may be due to gender-associated differences in behavior, there is overwhelming evidence that sex-associated hormones can also modulate immune responses and consequently directly influence the outcome of parasitic infection. Animal models of disease can often recreate the gender-dependent differences observed in humans, and the role of sex-associated hormones can be confirmed by experimentally altering their levels. Under normal circumstances, levels of sex hormones not only differ between males and females but vary according to age. Furthermore, not only are females of reproductive age subject to the regular hormonal cycles which control ovulation, they are also exposed to dramatically altered levels during pregnancy. It is thus not surprising that the severity of many diseases, including those caused by parasites, has been shown to be affected by one or more of these circumstances. In addition, infection with many pathogens has been shown to have an adverse influence on pregnancy. In this article we review the impact of sex-associated hormones on the immune system and the development and maintenance of immunity to the intracellular protozoan parasites Toxoplasma gondii, Plasmodium spp., and Leishmania spp

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Effect of 17β-oestradiol on expression of IL-2, IL-6 and IFN-γ mRNA in human tonsillar cells

Evagelatou

Farrant

1995

Endocr

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Oestradiol (E(2)) alters lymphocyte functionin vitro including T cell DNA synthesis and B cell immunoglobulin production in human tonsillar, splenic and peripheral blood cells. We have investigated whether one mechanism for this effect is that E(2) modifies the expression of IL-2, IL-6 and IFN-γ mRNA in human tonsillar mononuclear cells. Without E(2), addition of PHA (1 μg ml(-1)) for 10 h increased the expression of IL-2 and IL-6 mRNA but had no significant effect on IFN-γ mRNA. In separated T cells after 24 h incubation, E(2) (7×10(-8) M: ) increased only the IFN-γ mRNA levels. However, when E(2) was present in PHA-stimulated T cell cultures, mRNA levels from all cytokines were suppressed. E(2) decreased IL-2 mRNA levels in the T cell preparation after 24 h culture. For IL-6, E(2) decreased mRNA both in mononuclear cells and T cells after 10 h incubation. For IFN-γ, E(2) decreased mRNA levels in the mononuclear cell preparation after 24 h culture. Stimulation of the T cell preparation with PHA after 24 h incubation with E(2) decreased the IFN-γ mRNA levels compared to the cultures incubated with E(2) only. One part of the action of E(2) may be through a block in the up-regulation of the mRNA of IL-2, IL-6 and IFN-γ in activated cells.

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Effect of 17β-estradiol on immunoglobulin secretion by human tonsillar lymphocytes In vitro

Cited by 13 publications

References 19 publications

Effect of oestradiol-17β on the expression of oestrogen receptor mRNA in human tonsillar cells

Effect of oestradiol-17β on the expression of oestrogen receptor mRNA in human tonsillar cells

Sex-Associated Hormones and Immunity to Protozoan Parasites

Effect of 17β-oestradiol on expression of IL-2, IL-6 and IFN-γ mRNA in human tonsillar cells

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