Effect of 2-Deoxy-D-Glucose on Differentiation of Herpetomonas samuelpessoai

Angluster, Jayme; Mm, Bunn; W, de Souza

doi:10.2307/3279911

Cited by 33 publications

(13 citation statements)

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“…Non‐pathogenic trypanosomatids have emerged as important models for the study of basic biological processes performed by an archetypal eukaryotic cell. In this context, several Brazilian researchers have used Herpetomonas samuelpessoai , a monoxenous trypanosomatid, as a very interesting model to study physiological, genetic, biochemical, and ultrastructural aspects of flagellate trypanosomatids [Angluster et al, 1977; McGhee and Cosgrove, 1980; Soares et al, 1984; Santos and Soares, 2007]. H. samuelpessoai was originally isolated from the predatory insect Zelus leucogramus (Hemiptera: Reduviidae) [Galvao et al, 1970], which can be easily cultivated either in complex or chemically defined medium at 26 °C (insect vector temperature) or 37 °C (mammalian temperature) [Roitman et al, 1972].…”

Section: Introductionmentioning

confidence: 99%

Effects of direct electric current on Herpetomonas samuelpessoai: An ultrastructural study

Gomes

Teixeira

Barbosa

et al. 2011

Bioelectromagnetics

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The literature shows that the effects of direct electric currents on biological material are numerous, including bactericidal, fungicidal, parasiticidal, and anti-tumoral, among others. Non-pathogenic trypanosomatids, such as Herpetomonas samuelpessoai, have emerged as important models for the study of basic biological processes performed by a eukaryotic cell. The present study reports a dose-dependent anti-protozoan effect of direct electric treatment with both cathodic and anodic current flows on H. samuelpessoai cells. The damaging effects can be attributable to the electrolysis products generated during electric stimulation. The pH of the cell suspension was progressively augmented from 7.4 to 10.5 after the cathodic treatment. In contrast, the anodic treatment caused a pH decrease varying from 7.4 to 6.5. Transmission electron microscopy analyses revealed profound alterations in vital cellular structures (e.g., mitochondrion, kinetoplast, flagellum, flagellar pocket, nucleus, and plasma membrane) after exposure to both cathodic and anodic current flows. Specifically, cathodic current flow treatment induced the appearance of autophagic-like structures on parasite cells, while those submitted to an anodic current flow presented marked disorganization of plasma membrane and necrotic appearance. However, parasites treated in the intermediary chamber (without contact with the electrodes) did not present significant changes in viability or morphology, and no pH variation was detected in this system. The use of H. samuelpessoai as a biological model and the direct electric current experimental approach used in our study provide important information for understanding the mechanisms involved in the cytotoxic effects of this physical agent.

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Section: Introductionmentioning

confidence: 99%

Effects of direct electric current on Herpetomonas samuelpessoai: An ultrastructural study

Gomes

Teixeira

Barbosa

et al. 2011

Bioelectromagnetics

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“…The major generic character of Herpetomonas is the presence of an opisthomastigote form in culture or in the insect host (Hoare and Wallace 1966;Janovy 1974;McGhee and Cosgrove 1980). Previous studies have shown that some drugs binding to the plasma membrane, such as 2deoxy-D-glucose (Angluster et al 1977), concanavalin-A (Con A) (Souza et al 1980), dimethylsulfoxide (Castellanos et al 1981), propranolol (Lopes et al 1983), and lipopolysaccharide (Fiorini et al 1985), induce a cell dierentiation process in Herpetomonas samuelpessoai. These substances induce changes in cell surface saccharide composition (Alviano et al 1981;Soares et al 1984) and in cell surface charge (Soares et al 1988;Lopes et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Cell surface composition of promastigote and opisthomorph forms of Herpetomonas roitmani (Kinetoplastida: Trypanosomatidae)

Faria-e-Silva

Silva-Filho

Souza

1999

Parasitology Research

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Cell surface saccharide composition and surface charge of promastigote (PRO) and opisthomorph (OPM) forms of Herpetomonas roitmani were analyzed using labeled lectins and flow cytometry and cell electrophoresis. The FITC signals for concanavalin A, Helix pomatia agglutinin and wheat germ agglutinin were stronger in PRO forms, whereas for Limulus polyphemus agglutinin (LPA) and Wisteria floribunda agglutinin they were stronger in OPM forms. Prior treatment of the cells with neuraminidase decreased the FITC signal for LPA in OPM but not in PRO forms. Furthermore OPMs displayed a high negative charge (-15.45+/-1.10 mV) than PROs (-9.47+/-1.01 mV). Neuraminidase and phospholipase C treatment of the parasites significantly reduced the surface charge, especially in OPM forms. TLC analysis of the acidic components of H. roitmani showed the presence of N-acetyl-neuraminic acid. The results presented in this work indicate that changes in exposed cell surface components occur between PRO and OPM forms of H. roitmani obtained by growing the cells under different conditions.

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“…Promastigotes of H samuelpessoai, H. muscarum muscarum, H. megaseliae and H. roitmani display a negative surface charge, with mean EPM varying, according to the species analyzed (Table I). Treatment of Herpetomonas with 2-deoxy-D-glucose (Angluster et al 1977), concanavalin-A (ConA) (Souza et al 1980), dimethylsulfoxide (DMSO) (Castellanos et al 1981), propranolol (Lopes et al 1983) and lipopolysaccharide (LPS) (Fiorini et al 1985) triggers the process of cell differentiation from promastigote to para-and opisthomastigote forms, affecting the composition of membrane-associated polysaccharides (Alviano et al 1981, Soares et al 1984, cell surface charge (Soares et al 1988, Lopes et al 1989) and cell respiration (Fiorini et al 1985). Dimethiylsulfoxide and propranolol caused a significant increase in the net negative surface charge of both H. samuelpessoai and H. muscarum muscarum due to a markedly increase in the number of Nacetylneuraminic acid residues per cell (Soares et al 1988, Lopes et al 1989.…”

Section: Cellular Electrophoretic Mobilitymentioning

confidence: 99%

The surface charge of trypanosomatids

Souto-Padrón

2002

An. Acad. Bras. Ciênc.

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The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.

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Effect of 2-Deoxy-D-Glucose on Differentiation of Herpetomonas samuelpessoai

Cited by 33 publications

References 0 publications

Effects of direct electric current on Herpetomonas samuelpessoai: An ultrastructural study

Effects of direct electric current on Herpetomonas samuelpessoai: An ultrastructural study

Cell surface composition of promastigote and opisthomorph forms of Herpetomonas roitmani (Kinetoplastida: Trypanosomatidae)

The surface charge of trypanosomatids

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