Effect of 5,7-dihydroxytryptamine on the concentration of individual proteins in different areas of the rat brain

Heydorn, William E.; Creed, G. Joseph; Nguyen, Khanh Q.; Jacobowitz, David M.

doi:10.1016/0006-8993(86)91062-0

Cited by 8 publications

(2 citation statements)

References 13 publications

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“…Protein synthesis is under the influence of serotonin in the LC neurons. Both the resuits of the present investigation as well as published data suggest this [12].…”

Section: Resultsmentioning

confidence: 48%

Target cells of serotoninergic innervation in the locus coeruleus

Otellin

Gilerovich

Mikhailova

1994

Neurosci Behav Physiol

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The noradrenergic and serotoninergic systems of the brain belong to the modulator system which determines the level of activity of various structures of the CNS. The functions in which serotonin and norepinephrine participate are numerous, and the disturbances associated with their dysbalance are diverse. Neuroendocrine regulation, cardiovascular control, the processes of breathing, learning and memory, emotions, the functions of sleep and waking, aggressiveness --this is far from a full list of the processes in which the action of serotonin and norepinephrine are associated [1-3, 5, 7, 19].Participation of these mediator systems in the pathogenesis of such illnesses as Parkinsonism, Alzheimer's disease, multiple sclerosis, and schizophrenia is well known [6,9,11,18,21,23].The interaction of the serotonin and noradrenergic systems determine to a substantial degree the level of functional activity of the brain. However, the ultrastructural bases of their interactions remain unknown at the present time. The purpose of the present investigation was the study of the targets of serotoninergic innervation in the locus coeruleus (CS), the principal noradrenergic center of brain. MATERIALS AND METHODSThe study was carried out in Wistar rats in which the serotoninergic system was selectively damaged by 5,7-dihydroxytryptamine (5,7-DHT). Desipramine, an inhibitor of the reverse uptake of catecholamines, was administered intraperitoneally 1 h before the injection of 5,7-DHT in order to block the influence of the preparation on the catecholamines. The animals were then administered 200 lag of 5,7-DHT dissolved in 10 ~ll of an isotonic sodium chloride solution, intracisternally. Animals which were administered an isotonic sodium chloride solution in the same volume intra- 457cisternaUy instead of 5,7-DHT served as the control. The animals were kept in the usual vivarium conditions. The material was taken from the experimental and control animals 2, 5, 7, 10 and 30 days, and 2 and 6 months following the administration of the preparation. The fixation was carried out by the perfusion of a 2.5% solution of glutaraldehyde in phosphate buffer (pH 7.2) and sucrose (0.136 M). The locus coeruleus was demonstrated under a binocular loupe in frontal sections. The postfixation was carried out with a 2% solution of osmium tetroxide. The subsequent processing of the tissues was carried out following conventional methods. The material was embedded in epon. Semithin sections were stained azure II. Ultrathin sections were obtained on an LKB ultratome and studied under an JEM-100B electron microscope at an accelerating voltage of 75 kV. RESULTS AND DISCUSSIONSeveral morphological types of neurons are distinguished in the LC. Some of the neurons are of large sizes, and have a large nucleus and nucleolus. The nuclear membrane contains numerous deep invaginations. The cytoplasm is samrated with organelles; the granular endoplasmic reticulum is well developed. Synapses on their bodies are characteristic for these neurons.The neurons of the secon...

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“…Protein synthesis is under the influence of serotonin in the LC neurons. Both the resuits of the present investigation as well as published data suggest this [12].…”

Section: Resultsmentioning

confidence: 48%

Target cells of serotoninergic innervation in the locus coeruleus

Otellin

Gilerovich

Mikhailova

1994

Neurosci Behav Physiol

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“…At the same time, Bm~ x of 3H-imipramine binding sites in platelets from patients with panic psychoses decreases, but K s remains unchanged [15]. Serotonin changes the activity of the genetic apparatus in nerve cells by modulating synthesis of some proteins [13], which mediate its effects in various cells [6]. Experiments with intracerebral administration of antibodies against serotonin-modulated protein SMP-69 (molecular weight 69 kDa, pI 6.0) to rats demonstrated that this agent is involved in the regulation of exploratory behavior [2] and consolidation of memory traces [3].…”

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confidence: 96%

Platelet content of serotonin-modulated protein SMP-69 in patients with schizophrenia

2000

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Enzyme immunoassay showed that serotonin-modulated protein SMP-69 from the brain of albino rats displayed no tissue specificity and was present in platelets. Platelet content of SMP-69 in patients with schizophrenia surpassed that in healthy humans. Our findings indicate that this method can be used in forensic psychiatry and clinical practice to control the state of patients in therapy.Key Words: platelets; serotonin-modulated protein schizophrenia; enzyme immunoassay In the 1950s, it has been proposed that serotonin play a role in the pathogenesis of schizophrenia [14]. Recent studies suggest that the pathogenesis of schizophrenia is associated with impaired serotonin metabolism in the brain [14]. Postmortem examination of the frontal cortex from patients with schizophrenia showed that the number of binding sites (Bx) for 3H-spiperone, a selective 5-HT 2 receptor ligand, decreased, while K d remained unchanged [5]. These data indirectly indicate the elevation of serotonin content in the frontal cortex in patients with schizophrenia.Noninvasive methods for estimating serotonin metabolism in brain cells of patients with schizophrenia are important for clinical practice. Parameters of serotonin metabolism in platelets, including the number of specific receptors and activity of enzymes involved in serotonin synthesis and degradation are similar to those in brain cells [9,10]. Therefore, platelets are a good model for analyzing serotonin metabolism in nerve cells in various brain diseases. The density (Bmax) for 5-HT 2 receptors in platelets from patients with depression and suicidal behavior increases [4,7,8]. At the same time, Bm~ x of 3H-imipramine binding sites in platelets from patients with panic psychoses decreases, but K s remains unchanged [15]. Serotonin changes the activity of the genetic apparatus in nerve cells by modulating synthesis of some proteins [13], which mediate its effects in various cells [6]. Experiments with intracerebral administration of antibodies against serotonin-modulated protein SMP-69 (molecular weight 69 kDa, pI 6.0) to rats demonstrated that this agent is involved in the regulation of exploratory behavior [2] and consolidation of memory traces [3]. Here we measured the content of SMP-69 in platelets from patients with psychotic schizophrenia. MATERIALS AND METHODSTotal proteins of the liver, testes, brain, pancreas, and platelets from 4 rats were extracted in a homogenizer in 0.05 M phosphate buffer (pH 7.2) containing 0.3 M NaC1, 5 mM ethylenediaminetetraacetate (EDTA), and 0.1% Triton X-100.The blood (2 ml) from the ulnar vein of patients with psychotic schizophrenia (n=23) and healthy subjects (n=15) was collected into tubes with 300 btl 5% EDTA preventing coagulation and centrifuged at 1500 rpm for 5 min. The plasma was collected, platelets were precipitated by centrifugation at 6000 rpm for 10 min, and proteins were extracted by the above described method. The content of SMP-69 in protein extracts was measured by enzyme-linked immunosorbent assay (ELISA) on polystyrene p...

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The serotoninergic innervation of the area postrema

Semenov

1993

Neurosci Behav Physiol

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Effect of 5,7-dihydroxytryptamine on the concentration of individual proteins in different areas of the rat brain

Cited by 8 publications

References 13 publications

Target cells of serotoninergic innervation in the locus coeruleus

Target cells of serotoninergic innervation in the locus coeruleus

Platelet content of serotonin-modulated protein SMP-69 in patients with schizophrenia

The serotoninergic innervation of the area postrema

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