Effect of 5-aminoimidazole-4-carboxamide ribonucleoside on the mitochondrial function and developmental ability of bovine oocytes

Takeo, Shun; Abe, Takahito; Shirasuna, Koumei; Kuwayama, Takehito; Iwata, Hisataka

doi:10.1016/j.theriogenology.2015.03.013

Cited by 10 publications

(8 citation statements)

References 36 publications

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“…In previous reports, culturing bovine or porcine oocytes in medium containing resveratrol increased their ATP content [ 14 , 15 ]. Furthermore, when bovine oocytes were cultured with a potent activator of AMPK, 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), the ATP content increased [ 32 ]. In the present study, upregulation of ATP diminished by treatment with EX527, a SIRT1 inhibitor.…”

Section: Discussionmentioning

confidence: 99%

Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

Abe

Kawahara-Miki²,

Hara

et al. 2017

Journal of Reproduction and Development

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Resveratrol is a potent activator of NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and affects lipid metabolism and ATP generation in somatic cells. In the present study, the effects of supplementing culture medium with resveratrol on lipid metabolism, ATP generation, and cryosensitivity of bovine in vitro produced embryos were investigated. Bovine early cleaved-stage embryos were cultured in medium containing 0 or 0.5 µM resveratrol for 1 or 5 days. Resveratrol treatment for both 1 day and 5 days increased the expression levels of SIRT1 and phosphorylated AMP-activated protein kinase (pAMPK) in the embryos. Furthermore, resveratrol treatment was effective to increase ATP generation and reduce lipid content of the embryos. The effects of resveratrol treatment were diminished by the SIRT1 inhibitor “EX527”, and the reduced lipid content was reversed by treatment with etomoxir (a potent inhibitor of beta-oxidation). Blastocysts developed after resveratrol treatment showed low levels reactive oxygen species and increased cryotolerance. These results demonstrate that resveratrol improves in vitro development of bovine embryos, while reducing cytoplasmic lipid content through activation of beta-oxidation, thereby effective for production of bovine blastocysts with enhanced cryotolerance.

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Section: Discussionmentioning

confidence: 99%

Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

Abe

Kawahara-Miki²,

Hara

et al. 2017

Journal of Reproduction and Development

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“…Little information is available regarding age‐dependent changes in oviduct physiology. We addressed this deficiency by performing next‐generation sequencing to assess age‐dependent changes in post‐ovulatory bovine OECs from young (30–50 months old) and aged (>120 months old) cows (Takeo et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Oviductal tissues ipsilateral to the corpus luteum were collected at Days 1–3 after ovulation (which represents the phases of fertilization and early embryo development within the oviduct), transported to the laboratory (4°C, in phosphate‐buffered saline (PBS) containing antibiotics), and utilized for cell culture. In the present study, we defined cows of more than 120 months old as “aged,” and cows between 30 and 50 months old as “young” (Takeo et al, ). We used OECs from four young and four aged individuals for the in vitro studies.…”

Section: Methodsmentioning

confidence: 99%

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

et al. 2016

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The mammalian oviduct is an essential site for sperm storage, the transport of gametes, fertilization, and embryo development-functions that are aided by cytokines secreted from oviduct epithelial cells (OECs). Aging leads to cellular and organ dysfunction, with infertility associated with advanced maternal age. Few studies have investigated age-dependent changes in the oviduct as a possible cause of infertility, so we compared OECs from young (30-50 months) versus aged (more than 120 months) cattle. Next-generation sequencing was first used to identify age-related differences in gene expression. Several proinflammatory-related genes (including IL1B, IL1A, IL17C, IL8, S100A8, S100A9, and TNFA) were activated in OECs from aged (more than 120 months) compare to young (30-50 months) individuals, whereas genes associated with extracellular matrix-related factors (COLs, POSTN, BGN, and LUM) were down-regulation in aged OECs. Indeed, IL1 B and IL8 abundance was higher in aged OECs than in young OECs. Young OECs also tended to proliferate faster, and the revolution frequency of young, ciliated OECs was higher than that of their aged counterparts. In contrast, aged OECs possessed more F-actin, an actin cytoskeleton marker associated with reduced elasticity, and contained high levels of reactive oxygen species, which are mediators of inflammation and senescence. These different functional characteristics of bovine OECs during the post-ovulatory phase support the emerging concept of "inflammaging," that is, age-dependent inflammation. Mol. Reprod. Dev. 83: 815-826, 2016 © 2016 Wiley Periodicals, Inc.

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“…Immunostaining of oocytes was conducted as previously described [ 25 , 30 ]. Briefly, oocytes were fixed in 4% paraformaldehyde-PBS for 12 h, then permeabilized with PBS-PVA containing 0.25% Triton X-100 for 30 min at room temperature, followed by 1 h of incubation in blocking solution (PBS containing 5% BSA, 1% Tween 20, and 5% goat serum; GIBCO BRL, Paisley, UK).…”

Section: Methodsmentioning

confidence: 99%

Maternal aging affects oocyte resilience to carbonyl cyanide-m-chlorophenylhydrazone -induced mitochondrial dysfunction in cows

et al. 2017

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Mitochondrial quality control is important for maintaining cellular and oocyte viability. In addition, aging affects mitochondrial quality in many cell types. In the present study, we examined how aging affects oocyte mitochondrial biogenesis and degeneration in response to induced mitochondrial dysfunction. Cumulus oocyte complexes were harvested from the ovaries of young (21-45 months) and aged (!120 months) cows and treated for 2 hours with 10 μM carbonyl cyanide-m-chlorophenylhydrazone (CCCP), or a vehicle control, after which cumulus oocyte complexes were subjected to in vitro fertilization and culture. CCCP treatment reduced ATP content and increased reactive oxygen species (ROS) levels in the oocytes of both young and aged cows. When CCCP-treated cumulus oocyte complexes were subsequently cultured for 19 hours and/or subjected to fertilization, high ROS levels in oocytes and a low rate of blastocyst development was observed in oocytes derived from aged cows. In addition, we observed differential responses in mitochondrial biogenesis to CCCP treatment between young and aged cows. CCCP treatment enhanced mitochondrial biogenesis concomitant with upregulation of SIRT1 expression in oocytes of young, but not aged, cows. In conclusion, aging affects mitochondrial quality control and recuperation of oocytes following CCCP-induced mitochondrial dysfunction.

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Effect of 5-aminoimidazole-4-carboxamide ribonucleoside on the mitochondrial function and developmental ability of bovine oocytes

Cited by 10 publications

References 36 publications

Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

Maternal aging affects oocyte resilience to carbonyl cyanide-m-chlorophenylhydrazone -induced mitochondrial dysfunction in cows

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