Effect of 5-Aminolevulinic Acid on Leaf Senescence and Nitrogen Metabolism of Pakchoi Under Different Nitrate Levels

Wei, Zhen; Zhang, Zhi Ping; Lee, Marissa; Sun, Yajing; Wang, Liang Ju

doi:10.1080/01904167.2012.631666

Cited by 19 publications

(13 citation statements)

References 30 publications

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“…Furthermore, nutritive material uptake, such as sulfate or nitrate, could be strengthened by application of ALA. Nitrogen metabolism of watermelon seedling, which was affected by salinity, could be regulated through by ALA which significantly increased the activities of nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT), and glutamate dehydrogenase (GDH) (Chen et al 2017). In plants under nutrient deficiency condition, applied-ALA increased the transcription and translation level of NR or SULTR (a gene related to sulfur transport assimilation) (Maruyama-Nakashita et al 2010;Wei et al 2012;Beyzaei et al 2014).…”

Section: Amended Ions and Nutrients Uptakementioning

confidence: 99%

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

et al. 2018

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Crop productivity is restricted by various abiotic stresses such as drought, salinity, heat, and cold. Many efforts have been taken to decrease the inhibition of plant growth by alleviating the abiotic stresses. Exogenous applications of hormones, plant growth regulators, and/or small signaling molecules have been reported as a means to enhance plant resistance to stress. One of the small signaling molecules utilized is 5-aminolevulinic acid (ALA) that has been shown to enhance plant growth under abiotic stress. As a metabolic intermediate in higher plants, ALA is a precursor of all tetrapyrroles such as chlorophyll, heme and siroheme. The pathway towards biosynthesis upstream and the metabolism downstream of ALA contains multiple regulatory points that are affected by positive/negative factors. However, report about the regulatory aspects of the ALA metabolic pathway and the role of ALA in stimulating physiochemical processes in higher plants under stress have not been collated and summarized systematically. In this regard, we summarize recent developments in understanding the mechanisms of plant responses to abiotic stress which are affected by ALA as well as new information on the metabolic pathway of ALA. We find that exogenous application of ALA can enhance some key physiological and biochemical processes in plants such as photosynthesis, nutrient uptake, antioxidant characteristics and osmotic equilibrium, however, more in-depth research on the specific mechanisms are needed.

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Section: Amended Ions and Nutrients Uptakementioning

confidence: 99%

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

et al. 2018

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“…Increasing stomatal aperture indeed accelerates transpirational water loss, but transpiration simultaneously enhances water and nutrient uptake into plants, which is vital to plant growth. It has been reported that ALA significantly increased uptake of several nutrients, such as phosphate (Yao et al, 2006; Ali et al, 2014), nitrogen (Wei et al, 2012; Ali et al, 2014), sulfur (Ali et al, 2014), potassium calcium, magnesium, iron, copper, and zinc (Naeem et al, 2010; Zhang et al, 2015). 5-aminolevulinic acid also widely improves plant root activity and root growth (Kosar et al, 2015; An et al, 2016a,b), which is important for water and nutrient uptake and hence for plant growth and stress resistance (Sharp et al, 2003; Schachtman and Goodger, 2008).…”

Section: Discussionmentioning

confidence: 99%

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

Feng

Liu

et al. 2016

Front. Plant Sci.

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5-aminolevulinic acid (ALA), a new plant growth regulator, can inhibit stomatal closure by reducing H2O2 accumulation in guard cells. Flavonols are a main kind of flavonoids and have been proposed as H2O2 scavengers in guard cells. 5-aminolevulinic acid can significantly improve flavonoids accumulation in plants. However, whether ALA increases flavonols content in guard cells and the role of flavonols in ALA-regulated stomatal movement remains unclear. In this study, we first demonstrated that ALA pretreatment inhibited ABA-induced stomatal closure by reducing H2O2 accumulation in guard cells of Arabidopsis seedlings. This result confirms the inhibitory effect of ALA on stomatal closure and the important role of decreased H2O2 accumulation in this process. We also found that ALA significantly improved flavonols accumulation in guard cells using a flavonol-specific dye. Furthermore, using exogenous quercetin and kaempferol, two major components of flavonols in Arabidopsis leaves, we showed that flavonols accumulation inhibited ABA-induced stomatal movement by suppressing H2O2 in guard cells. Finally, we showed that the inhibitory effect of ALA on ABA-induced stomatal closure was largely impaired in flavonoid-deficient transparent testa4 (tt4) mutant. In addition, exogenous flavonols recovered stomatal responses of tt4 to the wild-type levels. Taken together, we conclude that ALA-induced flavonol accumulation in guard cells is partially involved in the inhibitory effect of ALA on ABA-induced H2O2 accumulation and stomatal closure. Our data provide direct evidence that ALA can regulate stomatal movement by improving flavonols accumulation, revealing new insights into guard cell signaling.

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“…Due to its various physiological roles and its natural, environmentally friend characteristics, increasing attention has been drawn on ALA and the mechanisms behind ALA's functions. Previous studies have indicated that ALA promotes plant growth including root biomass in both normal and stressful conditions (Ali et al 2013b(Ali et al , 2014An et al 2016d;Hotta et al 1997;Liu et al 2014Liu et al , 2016Wei et al 2012;Zhen et al 2012;Fig. 3 ALA modulates auxin transport components.…”

Section: Discussionmentioning

confidence: 99%

“…under normal conditions. Wei et al (2012) reported that ALA significantly promoted root growth when nitrate supply was in shortage. ALA also improved root growth of cucumber (Cucumis sativus L.) (Zhen et al 2012), Swiss chard (Beta vulgaris L. subsp.…”

Section: Introductionmentioning

confidence: 99%

5-Aminolevulinic acid (ALA) promotes primary root elongation through modulation of auxin transport in Arabidopsis

Cheng

Rao

et al. 2019

Acta Physiol Plant

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The specific function of 5-aminolevulinic acid (ALA), a new plant growth regulator, in modulating root growth of plants and the mechanisms underlying ALA-regulated root growth are largely unknown. Here, Arabidopsis seedlings were photographed and collected before and after ALA or 2,3,5-triiodobenzoic acid (TIBA) treatment for determination of root growth, fluorescence intensities of PIN1, PIN2, PIN7, and DR5, and gene expression levels of auxin synthesis, signaling, and transport. We first demonstrated that ALA significantly promoted Arabidopsis primary root elongation. We also found that TIBA, an auxin polar transport inhibitor, inhibited ALA-promoted root elongation, indicating that auxin transport is involved in ALAregulated root growth. Then, the observations of PIN1, PIN2, and PIN7 at protein and transcript levels suggest that ALA improves auxin transport mainly through regulating auxin efflux carriers. Furthermore, the expression patterns of auxinresponsive reporter DR5rev:GFP were not correlated well with the expression of YUC2, a key biosynthetic gene of auxin, but were consistent with changes of PIN1, PIN2, and PIN7. In addition, ALA did not affect the gene expression of auxin receptor, TRANSPORT-INHIBITOR-RESISTANT1 (TIR1). Taken together, we conclude that ALA promotes primary root elongation of young Arabidopsis seedlings mainly through improving auxin transport. Our data suggest the reciprocal interaction between ALA and auxin, providing new insights into the mechanisms underlying ALA-promoted plant root growth. Keywords 5-Aminolevulinic acid • Arabidopsis • Auxin • Polar transport • Root growth Communicated by B. Zheng. Yuyan An and Danxuan Cheng contributed equally to this work.

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Effect of 5-Aminolevulinic Acid on Leaf Senescence and Nitrogen Metabolism of Pakchoi Under Different Nitrate Levels

Cited by 19 publications

References 30 publications

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

5-Aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review

ALA-Induced Flavonols Accumulation in Guard Cells Is Involved in Scavenging H2O2 and Inhibiting Stomatal Closure in Arabidopsis Cotyledons

5-Aminolevulinic acid (ALA) promotes primary root elongation through modulation of auxin transport in Arabidopsis

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