Effect of 5'‐deoxy‐5'‐S‐isobutyl‐thioadenosine (SIBA) on the disposition of 5'‐methylthioadenosine by isolated rat hepatocytes

Inhibition of S-Adenosylhomocysteine Hydrolase by Acyclic Sugar Adenosine Analogue d-Eritadenine

“…Previous Chemical Modification Studies Are Consistent with the rWT:DEA Structure-Schanche et al (31) have characterized the AdoHcyase inhibitory activity of acyclic Ado analogues (DEA, L-eritadenine, L-threoeritadenine, and 9-(S)-(2,3-dihydroxypropyl)adenine). They have found that DEA is a potent inhibitor, whereas the others are moderate inhibitors.…”

“…On the other hand, most acyclic sugar Ado analogues except for DEA are relatively weak reversible inhibitors. DEA is a potent inhibitor of AdoHcyase (IC 50 ϭ 7 nM) and is believed to be an inactivator of AdoHcyase (31). As illustrated in Scheme 1, the chiral centers (C2Ј and C3Ј) of DEA have opposite chirality to those of Ado and cyclic sugar Ado analogues.…”

Evidence against a requirement for phospholipid methylation in adenylate cyclase activation by hormones

“…February 1982 cals and radioisotopes were from the sources in [19][20][21]. Male Wistar rats (,-,200 g) fed ad libitum were used.…”

“…to [methyl-all] methionine were homogenized in 1.2 M HCI, collected on glass-fiber filters, and washed and extracted as in [21 ] for determination of total phospholipid radioactivity. The butanol extract was analyzed by thin-layer chromatography [21 ], and >90% of the radioactive material chromatographed as phosphatidylcholine/lysophosphatidylcholine.…”

“…The butanol extract was analyzed by thin-layer chromatography [21 ], and >90% of the radioactive material chromatographed as phosphatidylcholine/lysophosphatidylcholine.…”