Effect of 5-Iodo-2'-Deoxyuridine on Multiplication of Rous Sarcoma Virus in vitro.

Abstract

Rous sarcoma virus (RSV) appears to contain ribonucleic acid (RNA), but not deoxyribonucleic acid (DNA) ( 1 ,2 ) . Recently, Temin(3) showed that Actinomycin D inhibits production of RSV, a finding which suggested that the growth of the RNA-virus is somehow associated with the synthesis of DNA. The present work indicates that 5iodo-2'-deoxyuridine ( IUdR) , an antagonist of DNA synthesis, not only inhibits RSV multiplication in primary cultures of chick embryo fibroblasts, but also appears to suppress the malignant transformation of the infected cells.Materials and methods. Compound. IUdR was obtained from the California Corporation for Biochemical Research.Tissue culture. The trunks of 10-day-old White Leghorn chick embryos, from RSVsusceptible flocks, were minced, washed with Scherer's solution and trypsinized with 0.25% trypsin (Difco, 1:250) for 5 minutes at room temperature. Tissue culture flasks (30 ml; Falcon Plastics) were seeded with 15 X lo6 cells contained in 5 ml of growth medium, which consisted of 30% medium 199, 60% Scherer's solution, 10% horse serum (heat-inactivated at 56°C for 40 minutes), pH 7.2, and 100 units of penicillin G and 1001 pg of streptomycin sulfate per ml. After incubation for 48 hours at 36"C, the cells were washed and refed for use with 5 ml of Scherer's solution (pH 7.2) containing 2.5% horse serum plus antibiotics (S-1).Virus. Standard stock Rous sarcoma virus, lot CT-929, was kindy supplied by Dr.

Variable effect of encephalomyocarditis virus on host defense mechanisms

“…The spleens were minced individually in RPMI 1640 medium and processed as described in the experiment for interferon induction. The cells were suspended at a concentration of 2 Delayed hypersensitivity to DNFB. The methods used in this series of experiments were described previously by Phanuphak et al (14).…”

Transformation by Murine Sarcoma Virus: Fixation (Deoxyribonucleic Acid Synthesis) and Development

“…Metabolic requirements for infection have not been reported for MSV. Reproduction of Rous sarcoma virus requires the synthesis of deoxyribonucleic acid (DNA) during the early stages of infection (1,7,14), and a similar requirement was observed for cellular transformation by Rous sarcoma virus (3). The synthesis of DNA has been implicated in the reproduction of other RNA-containing oncogenic viruses (2,10,20) and may be a requirement in the reproduction of all RNA tumor viruses.…”

Effect of Antiviral Agents in Equine Abortion Virus-Infected Hamsters

“…Hamsters and routes of treatment were selected because they had previously been reported protective in a number of other in vivo systems (6,14,20,23).…”