Plant growth regulators have significant effect on the delaying senescence in buds and flowers. Present study was conducted to find out the effects of Salicylic acid and Benzyl adenine spraying on various biochemical traits such as membrane stability index, chlorophyll a, and b content, total chlorophyll content, sugar content, catalase, and peroxidase enzymes activity, and proline content in Carnation. Study was conducted in a split plot design with three replications and various concentrations of hormones, cultivars of carnation and measurement time was used as a factor of interaction. Results of study showed that all the traits were significantly affected by spraying of these tow hormones. Cultivar factor was most effective in chlorophyll a, b, and total chlorophyll content, catalase enzyme activity, proline content and sugar content. Among these two hormones, spraying of Salicylic acid @ 100 µM was found most effective treatment. Further, time of measurements and cultivar factors were also found effective in all of traits.