Effect of 6-month caloric restriction on Cu bound to ceruloplasmin in adult overweight subjects

Abstract

In a randomized clinical trial of calorie restriction (CR), we demonstrated that important cardiovascular disease (CVD) biomarkers were favorably influenced by CR alone and in conjunction with physical exercise. The aim of this study was to examine the effects of CR with or without exercise on Copper bound to Ceruloplasmin (CuCp), a well-known biomarker for CVD, in overweight men and women enrolled in the CALERIE phase 1 study. Forty-six individuals were randomized to one of four groups for 6 months: control: healthy weight maintenance; CR: 25% CR from baseline energy requirements; CR + exercise: 12.5% CR and 12.5% through aerobic exercise; low calorie diet: low calorie diet until 15% reduction in body weight followed by weight maintenance diet. CuCp was determined in fasting blood samples by an HPLC-ICP-MS methodology and compared with changes in body composition and markers of CVD. After 6 months, CR combined with exercise induced a decrease in plasma concentration of CuCp. CuCp was inversely correlated with insulin sensitivity (Si) at baseline and after 6 months of intervention. A cluster analysis showed that the percent change of weight after 6 months of intervention was the most important variable that could discriminate the intervention groups. The percent change of CuCp was the only other variable selected by the analysis. Decreased CuCp in overweight subjects by CR combined with exercise suggest a positive effect of this intervention on metabolic health. Further studies to explain the relationship between weight loss and CuCp and its relevance for cardiovascular health are needed.

Is Caloric Restriction Associated with Better Healthy Aging Outcomes? A Systematic Review and Meta-Analysis of Randomized Controlled Trials

“…Finally, two studies, Racette 2006 [ 66 ] and Heilbronn 2006 [ 62 , 68 , 72 ], showed an improvement in insulin sensitivity that was associated with reductions in whole-body fat mass (r = −0.46; p = 0.001), visceral adipose tissue (r = −0.51; p < 0.01) and subcutaneous abdominal adipose tissue (r = −0.32; p < 0.05) [ 62 , 68 , 72 ].…”

Micronutrients impact the gut microbiota and blood glucose

“…In addition, copper-carrying proteins, such as ceruloplasmin, positively correlate with insulin resistance in subjects at increased diabetes risk (Aguilar et al 2013). Lifestyle modifications such as caloric restriction and physical exercise are associated with lower circulating levels of copper bound to ceruloplasmin in people that are overweight (Piacenza et al 2015). Overall, these results show a correlation between higher circulating copper levels and worse blood glucose control.…”