Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats

Abstract

Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2-(4-OH-3methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43mg/dL and 36.12mg/dL. The rats fed on highcholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p>0.05). 7-OH-2-(4-OH-3methoxyphenyl)-chroman-4-one with dose of 90mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.

In silico approach of bioactive molecule chitosan 501.1 kDa from snail shell as antioxidant and inhibitor of the keap1-nrf2 protein-protein interaction

“…Antioxidants are also a crucial part of the diet for hypercholesterolemia (Niki, 2012). However, endogenous antioxidants (SOD) in the body are limited in number to counteract free radicals, so the body cannot inhibit the rate of oxidative stress (Ayunda et al, 2019), but other antioxidant parameters are found in hypercholesterolemia conditions along with increased oxidative stress. Here, SOD is activated during the response to oxidative stress via the KEAP1/NRF2 pathway (Niedzielska et al, 2016;Zhuang et al, 2017).…”

Effects of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chroman-4-one on serum levels of antioxidant enzymes in hyperlipidemic rats

“…The high-fat diet for one week promotes hyperlipidemia as reviewed by Ayunda et al, 15 who reported that cholesterol-induced rats had higher total and low-density lipoprotein (LDL) cholesterol than the normal group. Lipids are very susceptible to damage caused by free radicals which result in lipid peroxidation that causes adverse changes.…”

Effect of Lissachatina fulica chitosan on the antioxidant and lipid profile of hypercholesterolemic male Wistar rats

“…The blood was left for 15 min and centrifuged for 10 min at 4000 rpm. The lipid profile parameters (total cholesterol, triglyceride, HDL-cholesterol, and LDL-cholesterol) were measured with the CHOP-PAP enzyme method (Ayunda et al, 2019).…”

“…However, the amount of endogenous antioxidants inside the body is limited in preventing free radicals. Therefore, the body cannot inhibit oxidative stress, and endogenous antioxidant is required (Ayunda et al, 2019). Chitosan from snail shells becomes one of the sources of exogenous antioxidants (Umarudin et al, 2019).…”