Academic performance of students is global issue of great concern to educators. This study sought to ascertain the effect of STEM problem-based learning as an inquiry approach on secondary school student's performance in Biology at government-owned secondary schools in Kebbi State, Nigeria. The non-equivalent control group design was adopted. Two schools were selected in which purposive sampling was employed to select the participants. Each class contain 40 participants. The data was obtained using Diffusion and Osmosis Achievement Test (DOAT). The variables were evaluated using descriptive statistics and the Independent Samples t-test. Based on the results obtained the STEM Problem-Based Learning (STEM-PBL) have a significant and favorable impact on students' biology achievement and that students in the experimental group retained more Biology information compared to the control group. The study recommended among others that, Biology classes should be made more engaging to students and teachers need to be more creative and inventive when it comes to discovering, selecting, and implementing activity-based instructional strategies in the classroom.Journal of Turkish Science Education 454 Student Assessment (PISA) (Chong, 2019). In Africa, academic achievement of Ghanaian students in biology has consistently remained poor (Annan et al., 2019). In Kenya, a steady decline in educational standards was observed among secondary school students (Mwaura et al., 2019).Most secondary school students in Nigeria misunderstands some biological concepts. They consider them difficult and challenging (Etobro & Fabinu, 2017). Students have misconceptions and difficulties in understanding and explaining the concepts of diffusion and osmosis (Do & Cerrah, 2018; Oladipo & Cynthia, 2018;Zita & Winifred, 2021). In most schools in Nigeria, biology is taught in abstract fashion and in disjointed form resulting in comprehension deficiency.In spite of all the instructional strategies employed by teachers, quite a number of students skipped and hardly attempt diffusion and osmosis related questions in West African Examination Council (WAEC), and if attempted are poorly answered (Yaki, et al., 2019). Biology achievement of students in WAEC in Nigeria was reported to be low in terms of credit level which qualifies students for admission into institutions of higher learning (Zita & Winifred, 2021). From 2016 to 2021, the percentage of secondary school students in Argungu Education Zone who received credit in biology was consistently lower than 50% (2016-2021 Credit % in Argungu Zonal Education Office).Secondary school classrooms in Nigeria were dominated by teacher-centred approaches. Conventional methods of instruction, according to Gorowara & Lynch, (2019), Iji, et al., (2015) and Samsudin, et. al., (2020), were the dominant strategies in teaching and learning of biology. Teaching and learning from a conventional approach as observed in many secondary school classrooms have contributed to low skilled students and poor academic achievement in...