Effect of a dark-colored substrate on the production of phycocyanin by the cyanobacterium Phormidium sp

Mastropetros, Savvas Giannis; Pispas, Konstantinos; Zagklis, Dimitris; Τσίγκου, Κωνσταντίνα; Ali, Sameh S.; Ariyadasa, Thilini U.; Kornaros, Michael

doi:10.1016/j.jece.2023.110580

Cited by 3 publications

(1 citation statement)

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“…More specifically, Tris-HCl pH 7, Tris-HCl pH 7.5, Tris-HCl pH 8, PB pH 6.5, DW, and tap water ( p = 0.232) can be used to extract the maximum yield of PC equal to 10.5 ± 0.9% w / w . In the study of Mastropetros et al [ 31 ], the percentage of the extracted PC, using deionized water and after the incubation of the lyophilized biomass of the same microorganism in the refrigerator for 24 h, was 0.9% w / w , indicating that the freeze–thaw method can significantly increase the extraction yield. The rolling two-way ANOVA of the results ( Figure S1b ) revealed no statistically significant difference ( p > 0.05), using different solvents for the second until the fourth FT cycle.…”

Section: Resultsmentioning

confidence: 99%

Optimizing Phycocyanin Extraction from Cyanobacterial Biomass: A Comparative Study of Freeze–Thaw Cycling with Various Solvents

Pispas,

Manthos,

Sventzouri

et al. 2024

Marine Drugs

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Cyanobacterial phycocyanin pigment is widely utilized for its properties in various industries, including food, cosmetics, and pharmaceuticals. Despite its potential, challenges exist, such as extraction methods impacting yield, stability, and purity. This study investigates the impact of the number of freeze–thaw (FT) cycles on the extraction of phycocyanin from the wet biomass of four cyanobacteria species (Arthrospira platensis, Chlorogloeopsis fritschii, Phormidium sp., and Synechocystis sp.), along with the impact of five extraction solutions (Tris-HCl buffer, phosphate buffer, CaCl2, deionized water, and tap water) at various pH values. Synechocystis sp. exhibited the highest phycocyanin content among the studied species. For A. platensis, Tris-HCl buffer yielded maximum phycocyanin concentration from the first FT cycle, while phosphate buffer provided satisfactory results from the second cycle. Similarly, Tris-HCl buffer showed promising results for C. fritschii (68.5% of the maximum from the first cycle), with the highest concentration (~12% w/w) achieved during the seventh cycle, using phosphate buffer. Phormidium sp. yielded the maximum pigment concentration from the first cycle using tap water. Among species-specific optimal extraction solutions, Tris-HCl buffer demonstrated sufficient extraction efficacy for all species, from the first cycle. This study represents an initial step toward establishing a universal extraction method for phycocyanin from diverse cyanobacteria species.

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Section: Resultsmentioning

confidence: 99%