Effect of a Dietary n‐6 Polyunsaturated Fatty Acid Supplement on Distinct Immune Functions of Goats

Rutten, Victor P.M.G.; Schonewille, J.T.; Hoek, A.; Beynen, A.C.; Noordhuizen, J.P.T.M.; Müller, Kerstin

doi:10.1111/j.1439-0442.2004.00595.x

Cited by 12 publications

(7 citation statements)

References 45 publications

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“…Even when not protected, dietary administered x-3 PUFAs are hydrogenated only to a small extent by rumen microorganisms (Ashes et al, 1992;Gulati et al, 1999). Therefore, EPA and DHA can be found in both blood and milk (Cattaneo et al, 2006), where they may contribute to modulate immune defences (Thanasak et al, 2004;Agazzi et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…While x-3 fatty acids are long since utilized as lipid sources in goat diets with the aim to increase the proportion of nutritionally beneficial x-3 PUFAs in milk for human consumption (Cattaneo et al, 2006), yet few studies have been carried out to investigate the effects of fish oil-derived PUFAs on leukocyte function in this species. The information available clearly evidenced an effect on leukocyte functions both in vivo (Agazzi et al, 2004;Thanasak et al, 2004;Bronzo et al, 2010) and in vitro on isolated neutrophils (Pisani et al, 2009) and monocytes (Lecchi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

Lecchi

Invernizzi

Agazzi

et al. 2013

Research in Veterinary Science

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a b s t r a c tThe present study investigated the in vitro effects on caprine monocytes of two x-3 PUFAs, namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on lipid droplet formation, an emerging process of fundamental importance in innate immunity regulation. The mRNA abundance of PAT protein family (PLIN1, PLIN2 and PLIN3), involved in the formation and trafficking of the droplets, was also assessed. The effects of EPA and DHA on monocyte apoptosis were studied as well. The number of lipid droplets per cell was found to be dependent on both type and concentration of fatty acid. x-3 PUFAs upregulated PLIN3 and PLIN2 gene expression, as well as apoptosis rate. The present findings suggest that PUFA might modify innate immune functions of goat monocytes by interfering with the formation of lipid droplets and by upregulating proteins belonging to PAT protein family.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

Lecchi

Invernizzi

Agazzi

et al. 2013

Research in Veterinary Science

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“…Supplementing n-3 PUFA to dairy goats altered the CD4+/CD8+ lymphocyte ratio during peripartum, favoring the CD4+ subset (T-helper lymphocytes) [107]. By contrast, the administration of corn oil rich in n-6 PUFAs reduced the surface expression of CD49d (α-4 integrin)—a subunit of lymphocytes homing receptors that modulate tissue-specific cell trafficking—in lymphocytes of male castrated goats [108].…”

Section: Fat Supplementation and Immune Responsementioning

confidence: 99%

Effects of Fat Supplementation in Dairy Goats on Lipid Metabolism and Health Status

Savoini

Zorini

Farina

et al. 2019

Animals

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Fat supplementation has long been used in dairy ruminant nutrition to increase the fat content of milk and supply energy during particularly challenging production phases. Throughout the years, advances have been made in the knowledge of metabolic pathways and technological treatments of dietary fatty acids (FAs), resulting in safer and more widely available lipid supplements. There is an awareness of the positive nutraceutical effects of the addition of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to fat supplementation, which provides consumers with healthier animal products through manipulation of their characteristics. If it is true that benefits to human health can be derived from the consumption of animal products rich in bioactive fatty acids (FAs), then it is reasonable to think that the same effect can occur in the animals to which the supplements are administered. Therefore, recent advances in fat supplementation of dairy goats with reference to the effect on health status have been summarized. In vivo trials and in vitro analysis on cultured cells, as well as histological and transcriptomic analyses of hepatic and adipose tissue, have been reviewed in order to assess documented relationships between specific FAs, lipid metabolism, and immunity.

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“…The principle of PBMCs isolation techniques are well recognized in many laboratories. The appropriated protocols have been set for many species, however, most of them are for mammal's PBMCs isolation [19][20][21][22]24]. Since, the reptiles are ectothermic animals which have variety of blood cell population, the appropriated protocols are necessary.…”

Section: Introductionmentioning

confidence: 99%

The peripheral blood mononuclear cells preparation and the hematology of Varanus salvator

2022

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The objectives of this study were to evaluate the proper anticoagulants coated in blood-collecting tube for the peripheral blood mononuclear cells (PBMCs) isolation and to evaluate the proper culture temperature for the Varanus salvator’s PBMCs, in addition, the hematological characteristics also reported. The heparin treated blood (n = 10) and EDTA treated blood (n = 10) from Varanus salvator were obtained for PBMCs evaluation. The PBMCs obtained from the heparin treated blood was significantly higher than that of EDTA treated blood during the culture period (P < 0.05) indicated heparin would be more appropriated anticoagulant for blood collection. The PBMCs cultured under 37°C and 27°C were not significantly difference on first three days but 37°C showed significantly higher effect in the following days (P < 0.05) indicated both temperatures can be used which 37°C should be an optimal for PBMCs preparation. The peripheral blood cells of Varanus salvator (n = 49) were analyzed for hematological profiles and characteristics which the number of erythrocytes 1.19 ± 0.04 x 1012/L (1.17–1.35 x 1012/L) and WBC 2.41 ± 0.13 x 109/L (2.29–2.81 x 109/L) with absolute differential count of heterophils 0.92 ± 0.02 x 109/L (0.87–0.95 x 109/L), lymphocytes 1.17 ± 0.01 x 109/L (1.15–1.23 x 109/L), azurophils 0.40 ± 0.01 x 109/L (0.37–0.42 x 109/L), basophils 0.000 ± 0.001 x 109/L (0.004–0.011 x 109/L) and monocytes 0.027 ± 0.002 x 109/L (0.028–0.039 x 109/L). These results would play an important role on the cell immunological studies of the Varanus salvator in the future.

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Effect of a Dietary n‐6 Polyunsaturated Fatty Acid Supplement on Distinct Immune Functions of Goats

Cited by 12 publications

References 45 publications

Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

Effects of EPA and DHA on lipid droplet accumulation and mRNA abundance of PAT proteins in caprine monocytes

Effects of Fat Supplementation in Dairy Goats on Lipid Metabolism and Health Status

The peripheral blood mononuclear cells preparation and the hematology of Varanus salvator

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