Effect of a functional feed additive on mitigation of experimentally induced gilthead sea bream Sparus aurata enteromyxosis

Palenzuela, Oswaldo; Pozo, R. del; Piazzon, M. Carla; Isern-Subich, M. M.; Ceulemans, Shana; Coutteau, Peter; Sitjà‐Bobadilla, Ariadna

doi:10.3354/dao03453

Cited by 16 publications

(9 citation statements)

References 41 publications

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“…This parasite lives and divides in the intestinal epithelium inducing intestinal damage, impaired nutrient absorption and anorexia. Therefore, among the most common disease signs induced by this parasite are diminished growth and weight loss [ 62 ]. After 70 days, post-infection (dpi) biometric parameters showed that, as expected, the weight gain, relative to the weight at 0 dpi, of the control uninfected fish was significantly higher in the fast-growth family (12.6%) than in the low-growth group (4.7%).…”

Section: Resultsmentioning

confidence: 99%

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Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

et al. 2020

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Background The key effects of intestinal microbiota in animal health have led to an increasing interest in manipulating these bacterial populations to improve animal welfare. The aquaculture sector is no exception and in the last years, many studies have described these populations in different fish species. However, this is not an easy task, as intestinal microbiota is composed of very dynamic populations that are influenced by different factors, such as diet, environment, host age, and genetics. In the current study, we aimed to determine whether the genetic background of gilthead sea bream (Sparus aurata) influences the intestinal microbial composition, how these bacterial populations are modulated by dietary changes, and the effect of selection by growth on intestinal disease resistance. To that aim, three different groups of five families of gilthead sea bream that were selected during two generations for fast, intermediate, or slow growth (F3 generation) were kept together in the same open-flow tanks and fed a control or a well-balanced plant-based diet during 9 months. Six animals per family and dietary treatment were sacrificed and the adherent bacteria from the anterior intestinal portion were sequenced. In parallel, fish of the fast- and slow-growth groups were infected with the intestinal parasite Enteromyxum leei and the disease signs, prevalence, intensity, and parasite abundance were evaluated. Results No differences were detected in alpha diversity indexes among families, and the core bacterial architecture was the prototypical composition of gilthead sea bream intestinal microbiota, indicating no dysbiosis in any of the groups. The plant-based diet significantly changed the microbiota in the intermediate- and slow-growth families, with a much lower effect on the fast-growth group. Interestingly, the smaller changes detected in the fast-growth families potentially accounted for more changes at the metabolic level when compared with the other families. Upon parasitic infection, the fast-growth group showed significantly lower disease signs and parasite intensity and abundance than the slow-growth animals. Conclusions These results show a clear genome-metagenome interaction indicating that the fast-growth families harbor a microbiota that is more flexible upon dietary changes. These animals also showed a better ability to cope with intestinal infections.

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Section: Resultsmentioning

confidence: 99%

“…For the parasite challenge, the sampling was performed 70 days post-infection (July 2018). All fish were starved for 48 h, sacrificed by overexposure to the anesthetic MS-222, weighted and the entire intestines were taken for parasite quantification by qPCR as previously described [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

et al. 2020

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show abstract

“…This parasite lives and divides in the intestinal epithelium inducing intestinal damage, impaired nutrient absorption and anorexia. Therefore, among the most common disease signs induced by this parasite are diminished growth and weight loss [62]. After 70 days post-infection (dpi) biometric parameters showed that, as expected, the weight gain, relative to the weight at 0 dpi, of the control uninfected sh was signi cantly higher in the fast-growth family (12.6%) than in the low-growth group (4.7%).…”

Section: Genetic Background Effects On Disease Resistancementioning

confidence: 53%

“…For the parasite challenge, the sampling was performed 70 days post-infection (July 2018). All sh were starved for 48 hours, sacri ced by overexposure to the anesthetic MS-222, weighted and the entire intestines were taken for parasite quanti cation by qPCR as previously described [62]. Foundation (FPCM).…”

Section: Sampling Proceduresmentioning

confidence: 99%

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

Piazzon

Naya-Català

Perera

et al. 2020

Preprint

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Background The key effects of intestinal microbiota in animal health has led to an increasing interest in manipulating these bacterial populations to improve animal welfare. The aquaculture sector is no exception and in the last years many studies have described these populations in different fish species. However, this is not an easy task, as intestinal microbiota is composed of very dynamic populations that are influenced by different factors, such as diet, environment, host age and genetics. In the current study, we aimed to determine whether the genetic background of gilthead sea bream ( Sparus aurata ) influences the intestinal microbial composition, how these bacterial populations are modulated by dietary changes, and the effect of selection by growth on intestinal disease resistance. To that aim, three different groups of five families of gilthead sea bream that were selected during two generations for fast, intermediate or slow growth (F3 generation) were kept together in the same open-flow tanks and fed a control or a well-balanced plant-based diet during nine months. Six animals per family and dietary treatment were sacrificed and the adherent bacteria from the anterior intestinal portion were sequenced. In parallel, fish of the fast- and slow-growth groups were infected with the intestinal parasite Enteromyxum leei and the disease signs, prevalence, intensity and parasite abundance were evaluated. Results No differences were detected in alpha diversity indexes among families, and the core bacterial architecture was the prototypical composition of gilthead sea bream intestinal microbiota, indicating no dysbiosis in any of the groups. The plant-based diet significantly changed the microbiota in the intermediate- and slow-growth families, with a much lower effect on the fast-growth group. Interestingly, the smaller changes detected in the fast-growth families potentially accounted for more changes at the metabolic level when compared to the other families. Upon parasitic infection, the fast-growth group showed significantly lower disease signs and parasite intensity and abundance than the slow-growth animals. Conclusions These results show a clear genome-metagenome interaction indicating that the fast-growth families harbor a microbiota that is more flexible upon dietary changes. These animals also showed a better ability to cope with intestinal infections.

show abstract

Section: Sampling Proceduresmentioning

confidence: 99%

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

Piazzon

Naya-Català

Perera

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The key effects of intestinal microbiota in animal health has led to an increasing interest in manipulating these bacterial populations to improve animal welfare. The aquaculture sector is no exception and in the last years many studies have described these populations in different fish species. However, this is not an easy task, as intestinal microbiota is composed of very dynamic populations that are influenced by different factors, such as diet, environment, host age and genetics. In the current study, we aimed to determine whether the genetic background of gilthead sea bream (Sparus aurata) influences the intestinal microbial composition, how these bacterial populations are modulated by dietary changes, and the effect of selection by growth on intestinal disease resistance. To that aim, three different groups of five families of gilthead sea bream that were selected during two generations for fast, intermediate or slow growth (F3 generation) were kept together in the same open-flow tanks and fed a control or a well-balanced plant-based diet during nine months. Six animals per family and dietary treatment were sacrificed and the adherent bacteria from the anterior intestinal portion were sequenced. In parallel, fish of the fast- and slow-growth groups were infected with the intestinal parasite Enteromyxum leei and the disease signs, prevalence, intensity and parasite abundance were evaluated. Results: No differences were detected in alpha diversity indexes among families, and the core bacterial architecture was the prototypical composition of gilthead sea bream intestinal microbiota, indicating no dysbiosis in any of the groups. The plant-based diet significantly changed the microbiota in the intermediate- and slow-growth families, with a much lower effect on the fast-growth group. Interestingly, the smaller changes detected in the fast-growth families potentially accounted for more changes at the metabolic level when compared to the other families. Upon parasitic infection, the fast-growth group showed significantly lower disease signs and parasite intensity and abundance than the slow-growth animals. Conclusions: These results show a clear genome-metagenome interaction indicating that the fast-growth families harbor a microbiota that is more flexible upon dietary changes. These animals also showed a better ability to cope with intestinal infections.

show abstract

Effect of a functional feed additive on mitigation of experimentally induced gilthead sea bream Sparus aurata enteromyxosis

Cited by 16 publications

References 41 publications

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

Genetic selection for growth drives differences in intestinal microbiota composition and parasite disease resistance in gilthead sea bream

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