Effect of a gonadotropin inhibiting substance on the ovarian histology in mice

Further studies on gonadotropin-inhibiting substances excreted in human urine.

“…A substance, so called gonadotropininhibiting substance (GIS), which inhibits the stimulatory effects of HCG on mouse uterine weight (Safer et al, 1961) and suppresses the ovulation induced by PMS and/or HCG in mice (Dronkert et al, 1963 ;Ota et al, 1968aOta et al, , 1968b has been found in pubertal and adult human urine. The inhibitor can be extracted from urine specimens according to the tannic acid precipitation method used for the extraction of gonadotropins excreted in the urine.…”

The Effect of a Gonadotropin Inhibiting Substance on Super-Ovulation Induced by Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin

“…The mice were dissected 18-20hrs. after the injection of HCG and the numbers of ova in the oviducts were counted and the ovaries and uteri were weighed as reported in the previous paper (Ota et al, 1967a). The suppression of ovulation by the injection of the inhibitor was considered as evidence of the presence of the inhibitor.…”