Effect of a hexacyclic triterpenic acid from Euscaphis japonica on the oleic acid induced HepG2 cellular model of non-alcoholic fatty liver disease

Chen, Yu; Zhang, Shaojun; Lu, Yanxia; Li, Lujun; Yu, Shuang; Lei, Yueyue; Yang, Xinyu; Wu, Zheng-Zhi

doi:10.1007/s00044-022-02982-z

Cited by 2 publications

(1 citation statement)

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“…It is important to identify the bioactive components present in EJF that contribute to the effects observed in this study. Previous studies have reported the presence of euscaphinin and euscapholide in the leaves [ 24 ], triterpene acid in the branches [ 25 ], and hexacyclic triterpenic acid in the roots [ 26 ] of E. japonica Kanitz. However, there have been no reports on the components present in the fruit of this plant.…”

Section: Discussionmentioning

confidence: 99%

Euscaphis japonica Kanitz Fruit Exerts Antiobesity Effects by Inhibiting the Early Stage of Adipogenic Differentiation

2023

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During the worldwide COVID-19 outbreak, there was an increase in the prevalence of obesity, including childhood obesity, due to which the awareness of obesity and interest in treatment increased. Accordingly, we describe EJF (Euscaphis japonica Kanitz fruit) extract as a candidate for naturally derived antiobesity agents. In this study, we found that EJF is involved in the early stage of adipogenic differentiation in vitro and finally inhibits adipogenesis. We propose two mechanisms for the antiobesity effect of EJF. First, EJF inhibits MDI-induced mitotic clonal expansion (MCE) by inducing cell cycle arrest at the initiation of adipogenic differentiation. The second aims to regulate stability and activation at the protein level of IRS1, which initiates differentiation in the early stage of differentiation. As a result, it was found that the activation of Akt decreased, leading to the inhibition of the expression of adipogenesis-related transcription factors (PPARγ, C/EBPα) and the subsequent suppression of adipogenic differentiation. In summary, we suggest that EJF can inhibit adipogenesis and lipid accumulation by suppressing the early stage of adipogenic differentiation in 3T3-L1 adipocytes. These findings indicate that EJF’s functionality could be beneficial in the treatment of obesity, particularly childhood obesity associated with adipocyte hyperplasia.

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Section: Discussionmentioning

confidence: 99%