Effect of a karst system (France) on extended spectrum beta-lactamase (ESBL)-producing Escherichia coli

Henriot, Charles P.; Celle, Hélène; Klaba, Victor; Biguenet, Adrien; Miège, Cécile; Daval, Amandine; Amiotte‐Suchet, Philippe; Beugnot, Jean‐Charles; Karbowiak, Thomas; Bertrand, Xavier

doi:10.1016/j.watres.2023.119582

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…However, this approach, too, does not resolve the differences between operational taxonomic units and the species level of a pathogen present. Whole‐genome sequencing is a more promising approach to tracking the source of a pathogen in a complex environment (Amirsoleimani et al., 2019 ; Flach et al., 2021 ; Henriot et al., 2023 ; Raza et al., 2021 ). Short‐read whole‐genome sequencing allows accurate reconstruction of the phylogenetic relationship of the sampled bacteria.…”

Section: Introductionmentioning

confidence: 99%

Local and Environmental Reservoirs ofSalmonella entericaAfter Hurricane Florence Flooding

Mao,

Zeineldin,

Usmani

et al. 2023

GeoHealth

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In many regions of the world, including the United States, human and animal fecal genetic markers have been found in flood waters. In this study, we use high‐resolution whole genomic sequencing to examine the origin and distribution of Salmonella enterica after the 2018 Hurricane Florence flooding. We specifically asked whether S. enterica isolated from water samples collected near swine farms in North Carolina shortly after Hurricane Florence had evidence of swine origin. To investigate this, we isolated and fully sequenced 18 independent S. enterica strains from 10 locations (five flooded and five unflooded). We found that all strains have extremely similar chromosomes with only five single nucleotide polymorphisms (SNPs) and possessed two plasmids assigned bioinformatically to the incompatibility groups IncFIB and IncFII. The chromosomal core genome and the IncFIB plasmid are most closely related to environmental Salmonella strains isolated previously from the southeastern US. In contrast, the IncFII plasmid was found in environmental S. enterica strains whose genomes were more divergent, suggesting the IncFII plasmid is more promiscuous than the IncFIB type. We identified 65 antibiotic resistance genes (ARGs) in each of our 18 S. enterica isolates. All ARGs were located on the Salmonella chromosome, similar to other previously characterized environmental isolates. All isolates with different SNPs were resistant to a panel of commonly used antibiotics. These results highlight the importance of environmental sources of antibiotic‐resistant S. enterica after extreme flood events.

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Section: Introductionmentioning

confidence: 99%

Local and Environmental Reservoirs ofSalmonella entericaAfter Hurricane Florence Flooding

Mao,

Zeineldin,

Usmani

et al. 2023

GeoHealth

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“…MLST analysis could be helpful in understanding the processes of genomic evolution in diverse species and the characteristics of a specific clone [ 22 ]. E. coli ST types are extraordinarily diverse, including ST10, ST48, ST95, and so on [ 23 ]. E. coli ST10, as a non-host-restricted pathogenic bacterium, can spread in humans and animals [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of an Escherichia coli Isolate Coharboring the Virulence Gene astA and Tigecycline Resistance Gene tet(X4) from a Dead Piglet

et al. 2023

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tet(X4) is the critical resistance gene for tigecycline degradation that has been continually reported in recent years. In particular, pathogenic bacteria carrying tet(X4) are a severe threat to human health. However, information describing Escherichia coli coharboring tet(X4) with virulence genes is limited. Here, we isolated an E. coli strain coharboring tet(X4) and the heat-stable toxin gene astA from a dead piglet. The strain named 812A1-131 belongs to ST10. The genome was sequenced using the Nanopore and Illumina platforms. The virulence genes astA and tet(X4) are located on the chromosome and in the IncHI1-type plasmid p812A1-tetX4-193K, respectively. The plasmid could be conjugatively transferred to recipient E. coli J53 with high frequency. In vivo experiments showed that strain 812A1-131 is pathogenic to Galleria mellonella and could colonize the intestines of mice. In summary, pathogenic E. coli could receive a plasmid harboring the tet(X4) gene, which can increase the difficulty of treatment. The prevalence and transmission mechanisms of pathogenic bacteria coharboring the tet(X4) gene need more attention.

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Antimicrobial resistance and karst groundwater systems: A policy gap analysis for addressing water quality and contaminants of emerging concern

Kaiser

Polk²,

Taing

et al. 2023

Environmental Science & Policy

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Effect of a karst system (France) on extended spectrum beta-lactamase (ESBL)-producing Escherichia coli

Cited by 3 publications

References 45 publications

Local and Environmental Reservoirs ofSalmonella entericaAfter Hurricane Florence Flooding

Local and Environmental Reservoirs ofSalmonella entericaAfter Hurricane Florence Flooding

Characterization of an Escherichia coli Isolate Coharboring the Virulence Gene astA and Tigecycline Resistance Gene tet(X4) from a Dead Piglet

Antimicrobial resistance and karst groundwater systems: A policy gap analysis for addressing water quality and contaminants of emerging concern

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