Effect of a medicinal extract from Agaricus blazei Murill on gene expression in a human monocyte cell line as examined by microarrays and immuno assays

Ellertsen, Linda K; Hetland, Geir; Johnson, Egil; Grinde, Bjørn

doi:10.1016/j.intimp.2005.07.007

Cited by 53 publications

(54 citation statements)

References 44 publications

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“…Interestingly, in CD also IL-17, which part-takes in the pathogenesis of the disease, and the Th-1 cytokine, IL-2, were reduced ( Figure 7). The decreased level of IL-17 disagrees with our finding of increased expression of the IL-23 gene in Andosan stimulated monocytic cells [48]. However, that was in vitro experiments which cannot be readily used for in vivo application.…”

Section: Citationcontrasting

confidence: 90%

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Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

Hetl

Therkelsen²,

Nentvich³

et al. 2015

J Clin Cell Immunol

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Andosan™ is an extract prepared from medicinal Basidiomycetes mushrooms, mainly Agaricus blazei Murill (AbM) (82%), but it also contains Hericeum erinaceus (He) (15%) and Grifola frondosa (Gf) (3%), all of which have immunomodulating properties. Whereas He and Gf have been used in traditional Chinese and Japanese medicine against cancer, AbM originates from Brazilian rain forest and was used locally and later abroad as health food for the prevention of a range of diseases, including chronic hepatitis, diabetes and cancer. The mushrooms' health effects are probably due to their content of β-glucans and other smaller immunomodulating polysaccharides and proteoglucans. These effects are mediated through their stimulation of innate immune cells, such as monocytes, NK cells and dendritic cells, and the amelioration of a skewded Th1/Th2 balance and of inflammation. Here, we give an overview of the anti-allergic effects of Andosan™ in a mouse model for allergy and anti-inflammatory effects both in vitro, in healthy individuals and in patients with inflammatory bowel diseases.

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Section: Citationcontrasting

confidence: 90%

“…In vitro stimulation of monocytic cells by Andosan AbM extract has shown increased expression of genes related to immune function [48]. Of special interest for our study of inflammatory bowel inflammation is upregulation of the gene for IL-23.…”

Section: Mechanism Of Action-immunomodulationmentioning

confidence: 99%

Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

Hetl

Therkelsen²,

Nentvich³

et al. 2015

J Clin Cell Immunol

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“…Up-regulation of IL8 has already been reported in pig PBMCs [39,40] and amnion [41] after bacterial infection. In human, stimulation of PBMCs with LPS induces the secretion of CCL2 [42], CXCL3 and CXCL2 [43]. CXCL5 is up-regulated in LPS-challenged bovine mammary epithelial cells [44].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response

et al. 2010

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BackgroundDesigning sustainable animal production systems that better balance productivity and resistance to disease is a major concern. In order to address questions related to immunity and resistance to disease in pig, it is necessary to increase knowledge on its immune system and to produce efficient tools dedicated to this species.ResultsA long-oligonucleotide-based chip referred to as SLA-RI/NRSP8-13K was produced by combining a generic set with a newly designed SLA-RI set that targets all annotated loci of the pig major histocompatibility complex (MHC) region (SLA complex) in both orientations as well as immunity genes outside the SLA complex.The chip was used to study the immune response of pigs following stimulation of porcine peripheral blood mononuclear cells (PBMCs) with lipopolysaccharide (LPS) or a mixture of phorbol myristate acetate (PMA) and ionomycin for 24 hours. Transcriptome analysis revealed that ten times more genes were differentially expressed after PMA/ionomycin stimulation than after LPS stimulation. LPS stimulation induced a general inflammation response with over-expression of SAA1, pro-inflammatory chemokines IL8, CCL2, CXCL5, CXCL3, CXCL2 and CCL8 as well as genes related to oxidative processes (SOD2) and calcium pathways (S100A9 and S100A12). PMA/ionomycin stimulation induced a stronger up-regulation of T cell activation than of B cell activation with dominance toward a Th1 response, including IL2, CD69 and TNFRSF9 (tumor necrosis factor receptor superfamily, member 9) genes. In addition, a very intense repression of THBS1 (thrombospondin 1) was observed. Repression of MHC class I genes was observed after PMA/ionomycin stimulation despite an up-regulation of the gene cascade involved in peptide processing. Repression of MHC class II genes was observed after both stimulations. Our results provide preliminary data suggesting that antisense transcripts mapping to the SLA complex may have a role during immune response.ConclusionThe SLA-RI/NRSP8-13K chip was found to accurately decipher two distinct immune response activations of PBMCs indicating that it constitutes a valuable tool to further study immunity and resistance to disease in pig. The transcriptome analysis revealed specific and common features of the immune responses depending on the stimulation agent that increase knowledge on pig immunity.

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“…It has been reported that ABM induces chemokine production, such as IL-8 or CXCL, by macrophages or monocytes [15,16]. During bacterial infection, neutrophils migrate toward the pathogen, with major chemotactic factors including C5a, leukotriene B 4 , Platelet-activating factor (PAF), and chemokines of the CXCL family [17].…”

Section: Discussionmentioning

confidence: 99%

Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils

Miyagawa¹,

Hirono²,

Kawazoe³

et al. 2013

JCDSA

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Hot water extract from the edible Brazilian mushroom, Agaricus Blazei Murill (ABM), is used for both traditional and alternative medicine. ABM is reported to stimulate anti-tumor, anti-infection, and immune activity. However, there are few reports of how ABM affects neutrophils. Therefore, in this study, we examined the effect of hot water ABM extract on neutrophil migration, phagocytosis, and reactive oxygen species production using neutrophils from guinea pig. Migratory direction and velocity as indicators of chemotactic activity of neutrophils were significantly (p < 0.001) increased at concentration of 50 and 100 mg/ml in ABM extract compared with control. Phagocytic activity of neutrophil was significantly (p < 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. Production of reactive oxygen species (ROS: H 2 O 2 or ) by neutrophils was significantly (p < 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. These results suggest that enhancement in neutrophil chemotactic activity, phagocytic activity and ROS production are mechanisms by which ABM extract inhibits bacterial infection in the skin and dermatitis.

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Effect of a medicinal extract from Agaricus blazei Murill on gene expression in a human monocyte cell line as examined by microarrays and immuno assays

Cited by 53 publications

References 44 publications

Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response

Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils

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