Effect of a New Amino Acid Solution on Nutritional Status and Nitrogen Metabolism in Rats with Chronic Renal Failure Undergoing Hyperalimentation.

Abstract

SummaryWe studied the effects of the new amino acid solution MRX-III on the nutritional status and nitrogen metabolism of rats with chronic renal failure (CRF) in comparison with those of a general amino acid solution (MPR-F). The essential amino acids/non-essential amino acids ratio was 3.21 for MRX-III and 1.09 for MPR-F. Rats with CRF, induced by 7/8 renal ablation, were divided into 6 groups of 8 rats each receiving total parenteral nutrition (TPN) containing MRX-III or MPR-F at a non-protein calorie/nitrogen ratio (Cal/N) of 300, 600 or 900 for 7 d. The rats were infused with test solutions containing the same amounts of non-protein calories. The cumulative nitrogen balance, as a nutritional index, in the MRX-III group was significantly higher than that in the MPR-F group at the Cal/N of 600 or 900, and the plasma albumin level at the Cal/N of 300. The plasma transferrin levels at the Cal/N of 900 in the MRX-III groups were significantly higher than those in the corresponding MPR-F groups. At all Cal/N, the MRX-III groups showed low levels of blood urea nitrogen and urinary excretion of ammonia and urea nitrogen as compared with the MPR-F groups at the same Cal/N. The plasma amino acid concentration profiles in the MRX-III groups after TPN showed greater similarity to that in the Normal group as compared with the profiles in the corresponding MPR-F groups. No aggravation of renal failure was observed in any TPN groups during TPN. These results indicate that, in rats with CRF undergoing hyperalimenta tion, the effects of MRX-III on the nutritional status and nitrogen metabolism are superior to those of the general amino acid solution, MPR-F. It is suggested that MRX-III could safely provide adequate amounts of nitrogen during hyperalimentation.

Total Parenteral Nutrition Using Continuous Intravenous Infusion via the Posterior Vena Cava in Rats

“…The serial time points of the blood biochemistry results in the TPN group are similar to the MAN group and our background data from orally fed rats. The blood biochemistry results in this study were similar to reported data for TPN rats (Mochida et al, 1991;Yamauchi and Kokuba, 1999). Since Al-P, T-Cho, TG, TP and ALB of our background data performed overnight fasting before blood biochemistry examinations, it showed a low value as compared with the blood biochemistry results in this study, but they were small differences.…”

“…Changes of all the blood biochemistry results in the TPN group showed results similar to MAN group. Blood biochemistry results in this study were within the limits of our background data, and were scientifically acceptable for TPN experiments (Mochida et al, 1991;Yamauchi and Kokuba, 1999). Although the overloading syndrome is reported to be a side effect in the early stages of TPN in rats (Silvis and Paragas, 1971), this syndrome is observed as increases in AST and ALT.…”

“…Most frequent causes in the failure of TPN are phlebitis, extravasations and hydrothorax (Fletcher and Little, 1988;Hecker et al, 1991). Moreover, there is no report by which the details of the serial data in blood biochemistry of TPN were clarified (Mochida et al, 1991;Yamauchi and Kokuba, 1999). In our previous study, we reported complications in that there are macroscopic and histopathological changes at the catheter tip of the injection site (Asanuma et al, 2002).…”

Contribution of Intestinal Calcium Absorption to 1,25-Dihydroxyvitamin D3-Induced Calcium Action in the Total Parenteral Nutrition Rat

“…However, no abnormality in this group was observed in the clinical signs. As shown in the blood biochemistry results (Tables 9 and 10), blood biochemistry results did not vary with MAN and TPN solution treatment rats, which is similar to the our background data in orally fed rats and TPN solution treatment rats (As anuma et al, 2006;M ochida et al, 1991;Yamauchi and Kokuba, 1999).…”

“…The blood biochemistry results did not vary with MAN and TPN solution treatment rats, which is similar to our background data in orally fed rats and TPN solution treatment rats (Asanuma et al, 2006;Mochida et al, 1991;Yamauchi and Kokuba, 1999).…”