Effect of a novel aromatic cytokinin derivative on phytochemical levels and antioxidant potential in greenhouse grown Merwilla plumbea

Abstract

In an attempt to elucidate the carry-over effect of cytokinins (CKs) on phytochemical and antioxidant activity of acclimatized plants, tissue culture-derived Merwilla plumbea supplemented with three CK types at four (0.25, 0.5, 1 and 2 lM) concentrations were grown for 6 months ex vitro. Phenolic acids including the hydroxybenzoic and hydroxycinnamic acid derivatives in M. plumbea were quantified using ultra performance liquid chromatography while the antioxidant activity was evaluated using oxygen radical absorbance capacity (ORAC). Different concentrations of gallic acid, protocatechuic acid, p-hydroxybenzoic acid and salicylic acid were observed with all the treatments with the exception of non-treated plants. Most phytochemicals (for example, gallic acid, ferulic acid protocatechuic acid and caffeic acid) were highest in plants obtained from 0.25 lM meta-topolin riboside (mTR). Likewise, plants derived from 2 lM mTR had the highest ORAC (684 lmol g -1 trolox equivalents) activity. Bearing in mind that therapeutic effects of medicinal plants are often associated to their phytochemical content, the current results are an indication on how the intricate in vitro environment (CK type and concentration in this case) affects the growth and general physiology of micropropagated plants especially after acclimatization.

Exogenous Application of Coconut Water to Promote Growth and Increase the Yield, Bioactive Compounds, and Antioxidant Activity for Hericium erinaceus Cultivation

“…Particularly, it has been observed that coconut water serves as a major stimulator in the production of triterpenoids and the accumulation of phenolic compounds. The present results suggest that coconut water has a notable impact on bioactive compound levels, particularly when interacting with phytohormones on bioactive compounds levels, which will affect antioxidant activity, and the research of Aremu et al [64] has shown that the production of higher content of phytochemicals with the appropriate cytokinin might be the key reason for the higher capacity to remove free radicals content. In another study, Yuan et al [54] conducted tests using methyl jasmonate-treated cultures of Sanghuangporus vaninii, showed higher activity in scavenging DPPH free radicals than those found in the control treatment.…”