Effect of a polysaccharide from  Poria cocos  on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines

Wu, Yajun; Li, Shuai; Li, Haixia; Zhao, Chunzhi; Ma, Haile; Zhao, Xiunan; Wu, Junhua; Liu, Kunlu; Shan, Jing; Wang, Yuxia

doi:10.1016/j.ijbiomac.2016.05.046

Cited by 60 publications

(23 citation statements)

References 23 publications

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“…Anti-viral activity has been reported in the past for exopolysaccharides [47]; however the mechanisms are not clearly understood. Perhaps, the most intriguing observation was the anti-proliferative effect on leukemia cells, HL60 and U-927 that were affected at different levels of the polymer.…”

Section: +mentioning

confidence: 99%

Structural Analysis and Physiological Properties of Exopolysaccharides Secreted by a Newly Isolated Pseudomonas stutzeri Bl58 under Alkaline Conditions

Yamada¹,

Yoshida²,

Nozaki³

et al. 2016

J Biotechnol Biomater

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We have identified an ethanol-assimilating bacterial strain of Pseudomonas stutzeri that produces copious amounts of an exopolysaccharide when cultured in 5% ethanol under the alkaline condition of pH 10. The objective of this study was to evaluate the potential industrial applications of this exopolysaccharide designated BL58 by characterization of its chemical, rheological and physiological properties. The polymer was purified from the 72 h culture broth with periodic feeding of ethanol and the chemical structure of the polymer was determined by NMR spectroscopy, GC-mass spectrometry and alditol-acetate analysis of methylated hydrolyzates. The analytical data showed the presence of glucose, mannose and 3-O-carboxyethyl rhamnose and suggested that the main chain consists primarily of 1,4-linked glucose and mannose units with unique branching of 3-O-[(R)carboxyethyl] rhamnose at the 3 position of the glucose unit, a structure not previously reported. The polysaccharide has an average molecular weight of 1,800 kDa and the rheological behavior of the aqueous solution from high viscosity sol to viscoelastic gel depended on temperature and pH. Although no overt cytotoxicity was observed, the polymer displayed anti-proliferative activity when tested with leukemia, liver carcinoma and normal pulmonary cells, indicating potential pharmaceutical applications.

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Section: +mentioning

confidence: 99%

Structural Analysis and Physiological Properties of Exopolysaccharides Secreted by a Newly Isolated Pseudomonas stutzeri Bl58 under Alkaline Conditions

Yamada¹,

Yoshida²,

Nozaki³

et al. 2016

J Biotechnol Biomater

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“…PCP-II has a molecular weight of 29.0 kDa, and it consists of fucose, mannose, glucose and galactose in a molar ratio of 1.00:1.63:0.16:6.29, respectively. PCP-II stimulated significantly antibody responses and extended the durable immunity to an inactiveted rabies vaccine, H1N1 influenza and HBsAg vaccine [33,34].…”

Section: Introductionmentioning

confidence: 98%

Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice

Gai

Zheng

Wang

et al. 2017

Virol J

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BackgroundMarburg virus (MARV) causes severe haemorrhagic fever in humans and nonhuman primates and has a high mortality rate. However, effective drugs or licensed vaccines are not currently available to control the outbreak and spread of this disease.MethodsIn this study, we generated MARV virus-like particles (VLPs) by co-expressing the glycoprotein (GP) and matrix protein (VP40) using the baculovirus expression system. MARV VLPs and three adjuvants, Poria cocos polysaccharide (PCP-II), poly(I:C) and aluminium hydroxide, were evaluated after intramuscular vaccination in mice.ResultsMurine studies demonstrated that vaccination with the MARV VLPs induce neutralizing antibodies and cellar immune responses. MARV VLPs and the PCP-II adjuvant group resulted in high titres of MARV-specific antibodies, activated relatively higher numbers of B cells and T cells in peripheral blood mononuclear cells (PBMCs), and induced greater cytokine secretion from splenocytes than the other adjuvants.ConclusionMARV VLPs with the PCP-II adjuvant may constitute an effective vaccination and PCP-II should be further investigated as a novel adjuvant.

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“…Wolfis are ergosterol, protein, lecithin, adenine, choline, histidine, fatty acid, and other inorganic elements. The main component regulating biologic activities are polysaccharide [13,14] and triterpenoid.…”

Section: Introductionmentioning

confidence: 99%

"Safety Evaluation of the Triterpenoid-Rich Extracts Derived from Poria Cocos"

2021

BJSTR

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The aim of this study was to evaluate the safety of the extracts of poria cocos, Tumulosic acid and Polyporenic Acid C, by acute oral toxicity test and micronucleus test for estimating the gene toxicity on ICR male mice. Our results revealed that there were no abnormal symptoms found in mice fed with Tumulosic acid (25 mg/ kg-BW) or Polyporenic Acid C (10 mg/ kg-BW) during the 14 day experimental period. The hematological analysis was within normal range and no obvious genotoxicity in micronucleus test of mice fed with indicated dosages of Tumulosic acid or Polyporenic Acid C compounds, and there was no significant difference among tissue weights of heart, liver, lung and kidney within each groups. However, we found that feeding of Tumulosic acid or Polyporenic Acid C resulted in decreased weight of immune organs, such as spleen and thymus. The histopathology demonstrated a mild lymphoid apoptosis in both the thymus and spleen. Collected data indicated that there were no toxic effect on murine fed with Tumulosic acid or Polyporenic Acid C compound in 14 days, but there might be immunotoxicity. Based on these findings, there seems to be a need for further studies.

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Effect of a polysaccharide from Poria cocos on humoral response in mice immunized by H1N1 influenza and HBsAg vaccines

Cited by 60 publications

References 23 publications

Structural Analysis and Physiological Properties of Exopolysaccharides Secreted by a Newly Isolated Pseudomonas stutzeri Bl58 under Alkaline Conditions

Structural Analysis and Physiological Properties of Exopolysaccharides Secreted by a Newly Isolated Pseudomonas stutzeri Bl58 under Alkaline Conditions

Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice

"Safety Evaluation of the Triterpenoid-Rich Extracts Derived from Poria Cocos"

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