Effect of a Single Intrauterine Dose of Human Recombinant Galectin-1 Buffered on Pregnancy Rate in Inseminated Cows

Abstract

The objective was to evaluate the efficacy of a single dose of exogenous galectin-1 in improving the pregnancy rate in inseminated cows, comparing the pregnancy rate of the two groups (treatment and control Groups) into 107 contemporary groups (YG) established. An ultrasound exam determined the pregnancy rate performed 25 to 35 days after the fixed-time artificial insemination (FTAI) of breeding beef cows (n = 3469). The pregnancy rate of cows that received a single dose of eGAL-1 (200 ± 10 µg), with an intrauterine administration (n = 1901), was compared with the pregnancy rate of cows inseminated using a conventional AI protocol (n = 1568), both comparing into the same YG. YGs were created considering the grouping of cows belonging to the same farm, with the same nutritional score and management, inseminated by the same inseminator and semen batch, and using the same estrus synchronization protocol). The statistical method used calculated the probability of obtaining pregnancy within each group. The administration of a single dose of eGAL-1 can increase the probability of obtaining pregnancy in beef cows by up to 8.68% (p < 0.0001), suggesting that a single dose of eGAL-1 during the FTAI procedure was reasonable in the beef cattle AI routine and can improve the pregnancy rate considerably.

In Vitro Bovine Embryo Production Using Medium’s Supplemented with Recombinant Human Galectin-1 Buffered

“…There are so many particularities between placentation and embryo elongation of different mammals' species but, based on [20,21,22], that described that GAL-1 has a high degree of structural conservation, dimerization, and binding properties with carbohydrates and integrins (adhesion proteins), suggesting that these properties are conserved among vertebrates and that they maintain a pattern of gene expression among the different types of the placenta (deciduous or not), supported the decision to use a human gene to produce the recombinant protein for other species. Previous studies have demonstrated the biological functionality of rHGAL-1 to improve the pregnancy rate in cows, suggesting the potential use in other species [1,2].…”

“…They suggested that blockage of GAL-1-mediated angiogenesis or lectin can disturb the processes associated with good placentation. Data from the authors (the improvement of the amount of GAL-1 into the bovine uterus) can be cited as a good example of impact of this protein in the pregnancy rate (1,2).…”

“…The method for obtaining rHGAL -1 is determined by the manufacturing process of Inprenha Biotecnologia® and is previously wholly described [1,2]. Aliquots of E. coli strains transformed with the vector insertion containing the GAL-1 gene (pET-29a(+)+lgals-1 gene 1 ) were grown in systems with LB Broth Base medium containing kanamycin sulfate until obtaining optimal bacterial growth rate, demonstrated by optical density.…”

“…Several immune cells with essential roles in the establishment and maintenance of pregnancy synthesize and respond to GAL-1, e.g., CD4+ CD25+ regulatory T-cell, which play a vital role in tolerating the immunogenic paternal alloantigens [5,1,11], and the regulation of the expression of human leukocyte antigen (HLA-G) in extravillous trophoblast, contributing to the tolerance via its interaction with immune and trophoblast cells [9].…”

“…Our research group recently evaluated the effect of a single intrauterine dose of human GAL-1 buffered on pregnancy rate in inseminated cows. The findings suggested that one dose of eGAL-1 was reasonable in the beef cattle artificial insemination routine and considerably improved the pregnancy rate [1,2]. Thinking about a future possibility of using the exogenous rHGAL-1 administration in human reproduction procedures (artificial insemination, embryo transfer, ex.…”