Effect of a Synthetic Indolicidin Analogue on Lipid Peroxidation in Thermal Burns

Abstract

Experimental simulation of burn was followed by accumulation of LPO products and suppression of antioxidant enzyme activity in the burn wound. Application of a synthetic analogue of indolicidin led to an increase in MDA and acylhydroperoxide concentrations in the burn wound on experimental day 1. Further application of the peptide in a dose of 100 mg/kg had no significant effect on the studied parameters, while the peptide in a dose of 500 mg/kg was followed by a decrease in the level of LPO products on days 10 and 14. Changes in antioxidant enzyme activities in rats treated with 500 mg/kg indolicidin analogue had a two-phase pattern: an increase on day 4 was followed by a decrease.

Naringenin improves the healing process of thermally-induced skin damage in rats

“…Statistically significant differences at *P < 0.05, ** P < 0.01 and *** P < 0.001 (one-way analysis of variance). NG (25), 25 mg/kg/day naringenin; NG(50), 50 mg/kg/day naringenin; NG(100), 100 mg/ kg/day naringenin Figure 2. Effect of oral naringenin treatment over 7 days following burn induction on wounded skin thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels, and on superoxide dismutase (SOD), catalase, glutathione-S-transferase (GST) and glutathione peroxidase (GPx) activity in male Wistar rats with thermally-induced burn injury.…”

“…Statistically significant differences at *P < 0.05, ** P < 0.01 and *** P < 0.001 (one-way analysis of variance). NG (25), 25 mg/kg/day naringenin; NG(50), 50 mg/kg/day naringenin; NG(100), 100 mg/kg/day naringenin well-vascularized dermis with many sebaceous glands and hair follicles (Figure 4(A)). Dermal sections of rat skin from day 7 of the sham-treated group showed scar formation, acanthosis, hyperkeratosis, vascular ectasia, epidermis separation from the dermis, oedema beneath the dermis, bleeding and severe inflammation within the dermis, a wide scar, inflammation beneath the scar and granular parakeratosis (Figure 4(B)).…”

“…24 Moreover, stimulated leukocytes are documented to produce free radicals, which in turn trigger the intensity of inflammation and lipid peroxidation. 25 , 26 …”

“…24 Moreover, stimulated leukocytes are documented to produce free radicals, which in turn trigger the intensity of inflammation and lipid peroxidation. 25,26 Phenolic compounds, including naringenin, are characterized by suppressive effects on cancerous cell proliferation, via inhibition of growth factor cascades, such as NF-kB. 27 In addition, naringenin has been shown to attenuate the induction of NF-kB following inactivation of TNF-a, irrespective of antioxidant properties.…”

The Study of Biochemical Parameters of Liver Mitochondria as Markers of Hypoxia in Burn Regeneration after Experimental Thermal Injury

“…There is also evidence of an increase of FRO processes of lipids and proteins with inhibition of the antioxidant systems in patients with thermal injury in the literature [16]. Reasons for the imbalance in the system of prooxidants-antioxidants may be the impairment of lipid and protein profile of mitochondria in thermal injury [17].…”