Effect of a thymic factor, thymostimulin, on growth and pulmonary metastases of Lewis lung carcinoma

Yagi, Minoru; Yamashita, Takashi; Tsubura, Eiro

doi:10.1007/bf00199226

Cited by 11 publications

(4 citation statements)

References 17 publications

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“…A suspension of 10 6 Preparation of effector cells. Spleen cells were harvested aseptically and gently crushed in RPMI 1640 with the flat end of a sterile syringe as described previously [31]. The cells were filtered through stainless-steel mesh and briefly rinsed in distilled water to lyze erythrocytes.…”

Section: Animalsmentioning

confidence: 99%

“…Mice bearing spontaneous pulmonary and lymph node metastases of Lewis lung carcinoma (3LL) cells were used [16,31]. To produce spontaneous metastases, 1 × l06 viable 3LL tumor cells in 0.05 ml RPMI 1640 were injected s.c. into one hind footpad of each mouse.…”

Section: Animalsmentioning

confidence: 99%

“…By this time, spontaneous pulmonary micrometastases had been established [16,31]. In our first experiments, a single i.p.…”

Section: Inhibition Of Spontaneous Pulmonary Metastases By Lentinan Amentioning

confidence: 99%

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Synergistic induction of lymphokine (IL-2)-activated killer activity by IL-2 and the polysaccharide lentinan, and therapy of spontaneous pulmonary metastases

Yamasaki

Sone

Yamashita

et al. 1989

Cancer Immunol Immunother

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Spleen cells of C57BL/6N mice bearing lung metastases were induced to the cytotoxic state by subcutaneous injection of recombinant human interleukin-2 (IL-2) at a minimum dose of 5 x 10(4) U/mouse three times a day for 3 consecutive days. A single intraperitoneal injection of lentinan alone at concentrations of up to 10 mg/kg body weight did not render spleen cells cytotoxic to P-29 cells, but a combination of subthreshold doses of these agents (5 x 10(4) U/ml IL-2 and 5 mg/kg lentinan) induced significant in vivo lymphokine-activated killer activity in spleen cells of tumor-bearing mice. Similarly, spleen cells from mice treated i.p. with lentinan became cytotoxic on in vitro treatment with IL-2. The in vitro responsiveness of spleen cells to IL-2 was maximal 3 days after i.p. injection of lentinan. Synergism between IL-2 and lentinan was also observed in mice bearing spontaneous lung micrometastases: neither IL-2 (less than 5 x 10(4) U/mouse) nor lentinan (less than 2.5 mg/kg) alone had a therapeutic effect, but multiple injections of IL-2 with a single injection of lentinan resulted in significant inhibition of spontaneous pulmonary metastases. From these results we conclude that IL-2 and lentinan in combination are more effective than either one alone for inducing destruction of pulmonary metastases.

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Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

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Synergistic induction of lymphokine (IL-2)-activated killer activity by IL-2 and the polysaccharide lentinan, and therapy of spontaneous pulmonary metastases

Yamasaki

Sone

Yamashita

et al. 1989

Cancer Immunol Immunother

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“…[24] Administering thymostimulin 10 mg/kg in combination with cyclophosphamide 50 mg/kg significantly prolonged the survival rate of mice with pulmonary metastases.l 24 ] In vitro assay indicated that the cytolytic activity of spleen cells against tumour cells was enhanced in mice treated with thymostimulin and/or cyclophosphamide. [24] Administering thymostimulin 10 mg/kg in combination with cyclophosphamide 50 mg/kg significantly prolonged the survival rate of mice with pulmonary metastases.l 24 ] In vitro assay indicated that the cytolytic activity of spleen cells against tumour cells was enhanced in mice treated with thymostimulin and/or cyclophosphamide.…”

Section: In Vivo Studiesmentioning

confidence: 99%

Thymostimulin

Dechant¹,

Bryson²

1994

Clin. Immunother.

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Suppressive effect of recombinant human Cu, Zn‐superoxide dismutase on lung metastasis of murtne tumor cells

Yoshizaki

Mogi

Muramatsu

et al. 1994

Intl Journal of Cancer

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The inhibitory effect of recombinant human Cu++Zn++superoxide dismutase (rhSOD) on metastasis of tumor cells in the mouse was investigated. In an experimental pulmonary metastasis model employing Meth A cells as inoculum, significant inhibition of metastasis was obtained by intravenous pre- and post-administration of rhSOD. An inhibitory effect of rhSOD was also observed in a spontaneous pulmonary metastasis model with 3LL cells as the inoculum. rhSOD was not observed to have any significant effects on the platelet-aggregating activity of tumor cells, the adhesiveness of tumor cells to vascular components (endothelial cells, laminin and type-IV collagen), or the growth of tumor cells either in vitro or in vivo. However, rhSOD suppressed invasion of Meth A and 3LL cells into Matrigel (an artificially reconstituted basement membrane of collagen, laminin and heparan sulfate) in the presence of hypoxanthine and xanthine oxidase, in vitro producers of superoxide. Thus, the present study shows that rhSOD is able to inhibit both experimental and spontaneous pulmonary metastasis, possibly through the suppression of tumor cell invasion into the extracellular matrix.

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Effect of a thymic factor, thymostimulin, on growth and pulmonary metastases of Lewis lung carcinoma

Cited by 11 publications

References 17 publications

Synergistic induction of lymphokine (IL-2)-activated killer activity by IL-2 and the polysaccharide lentinan, and therapy of spontaneous pulmonary metastases

Synergistic induction of lymphokine (IL-2)-activated killer activity by IL-2 and the polysaccharide lentinan, and therapy of spontaneous pulmonary metastases

Thymostimulin

Suppressive effect of recombinant human Cu, Zn‐superoxide dismutase on lung metastasis of murtne tumor cells

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