Effect of Abiotic Elicitors on Biochemical Status of Two Cultivars of Vigna Radiata (L.) Wilczek

Abstract

The present investigation has been conducted to analyse the effect of different concentrations of abiotic elicitors, Salicylic acid (0.01mM, 0.025mM and 0.05mM) and NaCl (50mM, 100mM and 150mM) individually and their combination on biochemical status of seeds of two cultivars, Pusa Vishal (PV) and SML 668 of Vigna radiata. All the biochemical analysis was conducted after 3 rd and 6 th day of imbibition. Highest amount of protein was observed in 100mM NaCl+0.025mM salicylic acid treated seeds of PV after 6 day as compared to all treatments as well as control set of both cultivars (PV and SML 668). Maximum proline content was recorded in 150mM NaCl treated seeds of PV after 3 rd day. Highest phenolic content was recorded in 150mM NaCl+0.025mM salicylic acid-treated seeds of SML 668 cultivar after 3 rd day. It was noticed that control seeds after 3 days showed higher phenolic content as compared to the 6 th day. In general, treatment of any elicitor individually or in combination, led to increase in phenolic content after 3 days but to decrease after 6 days. Similar results were obtained for proteins too. Any concentration of NaCl resulted in increase in proline content in both of the cultivars. All the results indicate that treatment with NaCl on PV and SML 668 seeds always led to increase in the concentration of proteins.