Effect of abscisic acid and slow drying on soluble carbohydrate content in developing embryoids of carrot (Daucus carota L.) and alfalfa (Medicago sativa L.)

Abstract

Effect of abscisic acid and slow drying on soluble carbohydrate content in developing embryoids of carrot ( Daucus carota L.) and alfalfa ( Medicago sativa L.) (1994). Effect of abscisic acid and slow drying on soluble carbohydrate content in developing embryoids of carrot ( Daucus carota L.) and alfalfa ( Medicago sativa L.). AbstractIn the presence of abscisic acid (ABA), contents of glucose and fructose decreased in carrot embryoids, whereas umbelliferose increased during dehydration The acquisition of desiccation tolerance was tested by scoring germinated embryoids It did not keep pace with the changes in soluble carbohydrate contents during development, which suggests that in these somatic embryoids di-and oligosacchandes are not the determining factors for anhydrobiosis However, on slow dehydration, umbelliferose increased, while sucrose decreased and the monosacchandes declined completely These changes were positively correlated with increased desiccation tolerance A similar analysis of slowly dried, ABA-treated alfalfa embryoids showed that stachyose and sucrose increased and the monosacchandes declined with the acquisition of desiccation tolerance These data support the contention that carbohydrates are involved in anhydrobiosis

Changed Properties of the Cytoplasmic Matrix Associated with Desiccation Tolerance of Dried Carrot Somatic Embryos. An in Situ Fourier Transform Infrared Spectroscopic Study1

“…It was reported previously that slow drying leads to the accumulation of the trisaccharide umbelliferose in carrot somatic embryos (Tetteroo et al, 1994(Tetteroo et al, , 1995. This accumulation does not occur when the embryos are subjected to fast drying.…”

“…Carrot (Daucus carota) somatic embryos can be rendered tolerant to severe desiccation by a proper combination of treatments (Tetteroo et al, 1994(Tetteroo et al, , 1995(Tetteroo et al, , 1996(Tetteroo et al, , 1998. Addition of ABA at the proper stage of development, a sufficient slow-drying time (at least 4 d), and a subtle rehydration are the main requirements for the acquisition of desiccation tolerance.…”

“…With fast drying the sugar composition is more or less maintained. However, with slow drying the trisaccharide umbelliferose increases at the expense of Suc, and the monosaccharides disappear almost completely (Tetteroo et al, 1994). Umbelliferose is a characteristic sugar in the Apiaceae family (Hopf and Kandler, 1976).…”

Water Content, Raffinose, and Dehydrins in the Induction of Desiccation Tolerance in Immature Wheat Embryos

“…The subsequent signal transduction chain in wheat embryos does not appear to be initiated specifically by ABA, as it can be in plant responses to water stress, because the signal (water loss) does not cause an increase in the ABA content. Nonetheless, ABA may participate, as the evidence suggests for other species such as barley and Arabidopsis (Bartels et al, 1988;Koornneef et al, 1989;Ooms et al, 1993;Tetteroo et al, 1994;Kermode, 1997). For now, other components of the transduction chain must remain the subject of speculation, but it is likely that they include factors that are being characterized with respect to the responses of vegetative tissues to drought and other stresses (Bohnert and Sheveleva, 1998).…”

Biological glasses: nature's way to preserve life

“…The liquid was evaporated under vacuum in a Speedvac AES1010 (Savant Instruments). The residue was dissolved in distilled water, and after appropriate dilution, sugars were analysed by HPLC on a Carbopac PA-1 column (Dionex Corp., Sunnyvale, CA, USA) using pulsed amperometric detection, as described by Tetteroo et al (1994). Data are the average of three extractions, each extraction containing 10 axes.…”