Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Pospíšilová, Jana; Synková, Helena; Haisel, D.; Baťková, P.

doi:10.1007/s10535-009-0003-5

Cited by 44 publications

(43 citation statements)

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“…Each parameters were measured before ex vitro transfer and after ex vitro transfer. Means ± SE, n = 20 propagated tobacco plantlets (Pospisilova et al 2009a). Higher chlorophyll a+b content was also observed when ABA was applied immediately after ex vitro transfer (Pospisilova et al 2009a).…”

Section: Resultsmentioning

confidence: 99%

“…Means ± SE, n = 20 propagated tobacco plantlets (Pospisilova et al 2009a). Higher chlorophyll a+b content was also observed when ABA was applied immediately after ex vitro transfer (Pospisilova et al 2009a). Chlorophyll a+b content in ABA-treated plants increased not only in persistent leaves but also new leaves developed at 2 weeks after ex vitro transfer (Pospisilova et al 1998).…”

Section: Resultsmentioning

confidence: 99%

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Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

2017

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Mulberry (Morus sp.) of the family Moraceae is very economically important in Asian countries including Korea, because its leaf and fruit have been commercially used in sericulture and horticultural industries. Therefore it is necessary to develop the optimal production system for rapid and cost-effective propagation of mulberry. Our studies focused on establishing an acclimatization method for the successful plantlet production of new cultivar 'Cheongsu' which was transferred ex vitro after in vitro culture. In particular, effect of abscisic acid (ABA) addition into the last subculture medium on plantlet response to subsequent ex vitro transfer and its growth was investigated. During acclimatization, stomatal conductance and transpiration rate of ABA-pretreated plantlets were significantly lower than those of non-treated plantlets. Net photosynthetic rate of ABA-pretreated plantlets decreased after ex vitro transfer but increased after 14 days, and it was mostly higher than that of non-treated plantlets. Moreover, relative water content as well as chlorophyll contents and its ratio were also higher in ABA-pretreated plantlets. On the other hand, proline was considerably higher than in control plantlets. After 1 month of ex vitro transfer, survival rate of ABA-pretreated plantlets was 85.6%, which increased by 29.1% in comparison with control (56.5%). More vigorous growth was also observed in ABA-pretreated plantlets. From these results, it was found that application of ABA to the last subculture medium could improve acclimatization and promote survival of mulberry plantlets after ex vitro transfer, inducing water stress tolerance and alleviating abiotic stresses.Keywords Mulberry, Abscisic acid, Ex vitro transfer, Acclimatization, Water stress Introduction Mulberry (Morus sp.) is an economically important tree grown in India, China, Korea and several Asian countries where its foliage is used as food for silkworms in sericulture industry (Vijayan et al. 2012). It is also commercially valuable in the horticulture, food and cosmetic industries, in particular, its phytochemical and medicinal properties such as antioxidants (Yen et al. 1996) and hypoglycemia compounds (Kelkar et al. 1996) have been widely used as healthcare products. These days it is cultivated for fruit production, which is used for human consumption including jam, jelly, frozen desserts, pulp, juice and wine (Koyuncu 2004). Mulberry fruit is regarded as a traditional medicine for dysentery, constipation and avulsed teeth due to rich phenolic acids and flavonoids (Arfan et al. 2012; Lee and Bae 2011).Plant micropropagation has been widely applied to agriculture industry for large scale production of economically important and valuable species. But its commercial use is limited in many species due to the low plantlet survival rates during the acclimatization (Pospisilova et al. 2009a, b), which are known to be related to an abnormal morphology, anatomy and physiology under in vitro culture conditions (Dias et al. 2013a). Poor functioning of...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

2017

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“…It has also been found to be a main physiological signal in inducing drought and high salinity responses (Farooq et al 2009 ;Lata and Prasad 2011 ). The role of ABA is not only in the regulation of developmental pathways but also for controlling many stress adaptation responses like activating genes responsible for osmotic adjustment, ion compartmentalization, root hydraulic conductivity, regulation of shoot and root growth, limiting transpiration rate and wilting, and ultimately reducing water loss in the plants (Verslues and Zhu 2005 ;Pospíšilová et al 2009 ). It is also involved in modifi cation of gene expression and upregulation of various stress-responsive genes during osmotic imbalance (Ingram and Bartels 1996 ).…”

Section: Role Of Aba and Transcription Factors In Osmotic Stress Signmentioning

confidence: 99%

Drought Stress Responses and Signal Transduction in Plants

Lata

Muthamilarasan

Prasad

2015

Elucidation of Abiotic Stress Signaling in Plants

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Nature provides all necessary components for healthy growth and development of plants in the form of air, water, light, nutrients, and soil. Any imbalance in the environmental harmony may cause stress to them. Stresses encountered by plants can broadly be categorized into biotic and abiotic stresses. Biotic stresses are mainly caused by pathogens and herbivory, whereas abiotic stresses include the threat imposed by drought, salinity, and extremes of temperature, heavy metals, and pollution. Drought stress is a major cause of yield instability in crops across diverse eco-geographic regions worldwide. A variety of biochemical, molecular, and physiological changes are manifested by plants in response to drought stress. The cellular abscisic acid (ABA) concentration increases on water defi cit leading to the activation of a number of stress-responsive genes and the patterns of expression of these genes are very complex, with some genes being induced early while others respond slowly. In general, drought-responsive genes respond to salt and cold stresses as well as to exogenous ABA treatment. However, there are several genes, which express themselves in an ABA-independent manner suggesting that both ABAdependent and -independent signal transduction cascades exist for drought stress perception, response, and adaptation. Drought stress response and adaptation in plants involves an array of pathways for signal perception, transduction, gene expression and synthesis of proteins, and other stress metabolites. Droughtresponsive genes can mainly be classifi ed into two groups. First group constitutes genes whose products provide osmotolerance and protection to plants thus directly functioning in tolerance to stress, while the second group includes genes playing a role in signal transduction as well as regulation of gene expression. This chapter summarizes the complex molecular mechanisms of drought stress response and adaptation in plants, highlighting the transcriptional regulation of stress-responsive

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“…In previous experiments ABA was applied immediately after ex vitro transfer of in vitro grown tobacco plantlets, and it was found that chlorophyll (a+b) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased. However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated plants (Pospíšilová et al 2009). A combined application of ABA and osmoticum is a routine method for stimulating somatic embryo maturation.…”

Section: Introductionmentioning

confidence: 99%

“…The relative water loss from detached leaves of in vitro grown Aronia arbutifolia, Tagetes erecta or tobacco plantlets was reduced by addition of ABA into culture medium (Colón-Guasp et al 1996, Pospíšilová 1996, Aguilar et al 2000. Addition of ABA to the substrate immediately after transplantation of in vitro grown tobacco plants also alleviated their wilting (Pospíšilová et al 1998(Pospíšilová et al , 2000(Pospíšilová et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

Pospíšilová¹,

Haisel²,

Synková³

et al. 2009

Biologia plant.

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Tobacco (Nicotiana tabacum L.) plantlets were grown on Murashige and Skoog medium in ventilated Magenta boxes and for the last subculture 10 μM ABA was added to the medium. After three weeks plantlets were transferred into pots with Perlite moistened with water and grown in controlled conditions (16-h photoperiod, day/night temperature 25/20 °C, air humidity about 45 %) either under low or high irradiance of 150 (LI) and 700 (HI) μmol m -2 s -1 , respectively. Content of endogenous ABA was 271.7 pmol g -1 (f.m.) in ABA treated plantlets, while in control plantlets it was only 53.3 pmol g -1 (f.m.). After ex vitro transfer, stomatal conductance and transpiration rate decreased considerably in comparison with in vitro grown plantlets and remained lower also 7 d after ex vitro transfer, especially in ABA-treated plants and so wilting of plants was practically eliminated. Net photosynthetic rate also decreased 1 d after ex vitro transfer but after 7 d it was mostly higher than that of in vitro grown plantlets. Water use efficiency significantly increased in ABA-treated plants. Chlorophyll a+b content did not change immediately after ex vitro transfer, nevertheless, after 7 d chlorophyll content was higher in ABA-treated plants. Pool of xanthophyll cycle pigments (XCP) and the degree of their deepoxidation (DEPS), which are connected with harmless dissipation of light energy, increased under high irradiance. Contents of XCP and ABA precursors (neoxanthin and violaxanthin) were lower in ABA-treated plants than in control plants indicating less stress in these plants. Most chlorophyll a fluorescence parameters did not change considerably after ex vitro transfer and so the photoinhibition was not observed even under HI. Slight increase in non-photochemical quenching under HI in ABA-treated plants suggested their better photoprotection. Thus application of ABA to the last subculture can improve acclimatization of in vitro grown plants to ex vitro conditions

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Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Cited by 44 publications

References 59 publications

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

Improvement ofex vitroacclimatization of mulberry plantlets by supplement of abscisic acid to the last subculture medium

Drought Stress Responses and Signal Transduction in Plants

Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

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