Effect of acid stress on protein expression and phosphorylation in Lactobacillus rhamnosus GG

Koponen, Johanna; Laakso, Kati; Koskenniemi, Kerttu; Kankainen, Matti; Savijoki, Kirsi; Nyman, Tuula A.; Vos, Willem M. de; Tynkkynen, Soile; Kalkkinen, Nisse; Varmanen, Pekka

doi:10.1016/j.jprot.2011.11.009

Cited by 133 publications

(131 citation statements)

References 70 publications

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…Under environmental stress conditions, LAB change metabolic and energy fluxes, modify the rate of growth, and adapt the metabolism of carbon sources to the new environment by modifying the synthesis of enzymes and metabolites (161). Environmental stresses inhibit the glycolytic pathway of Lc.…”

Section: Metabolism Of Carbon Sources and Energy Productionmentioning

confidence: 99%

See 1 more Smart Citation

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

Self Cite

539

404

View full text Add to dashboard Cite

SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

show abstract

Section: Metabolism Of Carbon Sources and Energy Productionmentioning

confidence: 99%

“…bulgaricus and Lb. rhamnosus under acid stress conditions (161,170). Acetyl-CoA is rerouted toward the biosynthesis of fatty acids instead of butanoate (161,170), which may enhance the rigidity and impermeability of the cytoplasmic membrane (177,178).…”

Section: Metabolism Of Carbon Sources and Energy Productionmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

Self Cite

539

404

View full text Add to dashboard Cite

show abstract

“…Conservation across species of phosphorylation sites in enzymes from the central carbon metabolism in pathways for glycolysis, pentose phosphate and the tricarboxylic acid (TCA) cycle, suggests that phosphorylation plays a pivotal role in regulating central carbon metabolism. Previous studies have also linked S/T/Y phosphorylation to regulation of cell differentiation [29], physiology [42,43], stress [44,45], virulence and pathogenicity [46][47][48][49][50].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions

Lim

Marcellin

Jacob

et al. 2015

Journal of Proteomics

View full text Add to dashboard Cite

Little is known about the role of global phosphorylation events in the control of prokaryote metabolism. By performing a detailed analysis of all protein phosphorylation events previously reported in Escherichia coli, dynamic changes in protein phosphorylation were elucidated under three different culture conditions. Using scheduled reaction monitoring, the phosphorylation ratios of 82 peptides corresponding to 71 proteins were quantified to establish whether serine (S), threonine (T) and tyrosine (Y) phosphorylation events displayed a dynamic profile under changing culture conditions. The ratio of phosphorylation for 23 enzymes from central carbon metabolism was found to be dynamic. The data presented contributes to our understanding of the global role of phosphorylation in bacterial metabolism and highlight that phosphorylation is an important, yet poorly understood, regulatory mechanism of metabolism control in bacteria. Biological significanceThe findings in this manuscript provide novel scientific knowledge about protein phosphorylation in dynamic regulation of the central carbon metabolism of E. coli. Evidence has accumulated confirming that phosphorylation is prevalently present in bacteria and has important regulatory roles of control of the central carbon metabolism. This investigation goes beyond data collections and shows that protein phosphorylation is quantitatively significant and varies under changing culture conditions.

show abstract

“…The response of lactobacilli to different stress conditions such as low pH, bile salt stress, high or low temperature stress, nutrient stress, etc., has been widely studied, due to their applications in healthy food and complementary medicines. [8][9][10][11] It has been suggested that impact on beneficial microbes by glyphosate may interfere with human and animal health, 2,7,12 although this is not necessarily a widely-held opinion. A number of recipes for so called "organic roundup" or "organic herbicide" have become widely distributed on the internet 13 as a way to address the perceived problems with glyphosate.…”

mentioning

confidence: 99%

Effects of Glyphosate or “Organic Herbicide” on Rumen Bacterial Isolates In Vitro

Jose¹,

Hussain²,

Bunt³

2017

Adv Food Technol Nutr Sci Open J

View full text Add to dashboard Cite

Glyphosate (N-(phosphonomethyl) glycine) is widely used by agriculture and has been claimed to potentially influence microorganisms; for example, Lactobacillus spp., which forms part of the normal microbiota of humans and animals. Comparing a commercial glyphosate containing herbicide against a recipe widely distributed on the internet, Kniss (2014) described as an organic herbicide the growth of five lactobacilli isolated from the bovine rumen and found little influence on rumen microbe isolates growth on agar plates. The growth of 5 isolates from the bovine rumen was not significantly influenced by glyphosate, a commercial glyphosate herbicide, and an organic herbicide and its components suggested that glyphosate influence is at most minor and likely to be of no consequence in vivo.

show abstract

Effect of acid stress on protein expression and phosphorylation in Lactobacillus rhamnosus GG

Cited by 133 publications

References 70 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions

Effects of Glyphosate or “Organic Herbicide” on Rumen Bacterial Isolates In Vitro

Contact Info

Product

Resources

About