Effect of Acid, Trypsin and Cold Treatment and of Renin— Plasma Interaction on the Activity of Renin Secreted by Rat Kidney

Nakane, Haruyuki; Nakane, Yoko; Corvol, P; Ménard, J

doi:10.1042/cs0570233

Cited by 14 publications

(5 citation statements)

References 16 publications

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“…The directional changes of active, inactive, and total renin following in vivo stimulation or suppression of the reninangiotensin system in the Wistar-Kyoto rats were, however, similar to the changes that have been reported to occur in other species. 14 The failure of either diuretic, metolazone or chlorthalidone, to significantly increase the plasma levels of active renin in the Wistar-Kyoto rat, even at these pharmacologically effective doses, was somewhat surprising. At equivalent dosage and duration of treatment, these drugs lower blood pressure in age-matched spontaneously hypertensive rats (141 ± 10.5 to 127 ± 7.9 mm Hg systolic pressure for metolazone and 144 ± 11.2 to 125 ± 7.3 mm Hg for chlorthalidone, unpublished data).…”

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confidence: 99%

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In vivo and in vitro alterations of active and inactive plasma renins in the rat.

Barrett¹,

Eggena²,

Sambhi³

1982

Hypertension

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SUMMARY The levels of active, inactive, and total renin (trypsin treatment) were measured in rat plasma before and after in vivo stimulation or suppression of active plasma renin. Stimulation of active renin was accompanied by either an increase (low sodium diet), no change (pentobarbital anesthesia plus hemorrhage), or fall (pentobarbital anesthesia) in the plasma levels of inactive renin, while suppression of active renin was accompanied by a fall (high sodium diet) or mild but nonsignificant Increases (clonldlne or saline infusion) of the inactive enzyme. These results suggest the possible independence of in vivo regulation of active and inactive renin in the rat.Trypsin However, the in vivo mechanism(s) that control the plasma levels of these enzymes has not been determined, and definitive information is not available as to whether the plasma levels of active and inactive renin can vary independently.In an attempt to determine whether alterations of active plasma renin can be related to changes in inactive renin, we used several antihypertensive agents and alterations in sodium diet to either stimulate or suppress active plasma renin. In addition, we fractionated plasma by isoelectric focusing techniques to determine: 1) which of the five isoelectrically distinct forms of active plasma renin 5 is altered following changes of active plasma renin; and 2) the isoelectric focusing distribution of plasma renins(s) that can be activated by trypsin. We used the rat as an experimental model since previous studies from this laboratory have indicated that rat plasma is a rich source of inactive renin as measured by both acid dialysis* and trypsin treatment. MethodsWistar Kyoto rats, bred in our laboratory, weighing from 150 to 200 g, were used for this study. Experimental diets were given for 2 weeks, consisting of Ralston Purina with a high sodium content (3.15% Na + ) and saline (0.9%) as drinking fluid, or with a low sodium content (0.05% Na + ) and distilled water as drinking fluid. Metolazone (5.0 mg/kg/day, s.q.), chlorthalidone (12.5 mg/liter drinking water), propranolol (320 mg/liter drinking water), and clonidine (280 ^g/liter drinking water) were also given for a period of 2 weeks with a normal sodium diet. The average daily water intake was approximately 30 ml per animal. A minimum of 10 animals was included in each study group. Animals were sacrificed by cervical dislocation, and blood was obtained from the left ven

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“…13 " 14 The relatively large proportion of inactive renin in plasma and its apparently low proportion in normal kidney tissue may indicate a postsecretory event or even plasma-mediated modification of the released enzyme. Preliminary evidence has indicated an acute increase of inactive renin following nephrectomy of the reninstimulated rat.…”

mentioning

confidence: 99%

In vivo and in vitro alterations of active and inactive plasma renins in the rat.

Barrett¹,

Eggena²,

Sambhi³

1982

Hypertension

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“…To summarize this section on renin 'activation': proteolytic enzymes can increase the renin concentration in human plasma and amniotic fluid in plasma from the pig (Boyd, 1974;Bailie, Derkx & Schalekamp, 1979) and rabbit but not the rat (Nakane, Nakane, Corvol & Menard, 1979), and in some extracts of human and animal kidneys. The effect of acid activation or cold treatment on plasma renin concentration is mediated at least partly through endogenous proteinases.…”

Section: Proteolytic Enzymesmentioning

confidence: 99%

Inactive Renin: An Attempt at a Perspective

Leckie

1981

Clinical Science

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“…Kidney perfusion in vitro was performed by previously described methods (Nakane, Nakane, Reach, Corvol & Menard, 1978;Nakane, Nakane, Corvol & Menard, 1979). The right kidney of male Wistar rats was perfused with a blood-free medium for 80 min, including a 20 min equilibration period.…”

Section: Kidney Perfusionmentioning

confidence: 99%

“…Acidification of renin samples was undertaken by previously described methods (Nakane et al, 1979). Briefly, samples were dialysed to p H 3.3 for 24 h and then re-dialysed to p H 6.5 for another 24 h.…”

Section: Acidification Of Renal Extract or Perfusate Satnplesmentioning

confidence: 99%

A Comparison of Multiple Forms of Renin in Rat Renal Perfusate with those in Renal Extract

Nakane

Misumi

et al. 1980

Clinical Science

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1. Physicochemical properties of renin secreted by isolated perfused rat kidney were examined and the results compared with those obtained for the renin in renal extract. 2. In renal extract, two high-molecular-weight renins (molecular weight 65 000 and 55 000) and one low-molecular-weight renin (molecular weight 39 000) were found. Their relative proportion varied depending on extraction conditions. By acidification, high-molecular-weight renins were converted into low-molecular-weight renin without marked changes in activity. 3. In renal perfusate only low-molecular-weight renin was found after renin stimulation by isoprenaline or anoxia. Inactive renin was not found. 4. Renin in renal extract and perfusate samples were both found to consist of at least four isoenzymes having different isoelectric points (pI). The pI patterns were identical in renal extract and perfusate samples: pI 5.7 (60-70%), 5.5 (15-25%), 5.3 (5-10%) and 5.0-5.2 (2-5%). 5. These results indicate that the native renin secreted by rat kidney consists entirely of the low-molecular-weight and active form comprising multiple isoenzymes with a stable pI pattern.

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Effect of Acid, Trypsin and Cold Treatment and of Renin— Plasma Interaction on the Activity of Renin Secreted by Rat Kidney

Cited by 14 publications

References 16 publications

In vivo and in vitro alterations of active and inactive plasma renins in the rat.

In vivo and in vitro alterations of active and inactive plasma renins in the rat.

Inactive Renin: An Attempt at a Perspective

A Comparison of Multiple Forms of Renin in Rat Renal Perfusate with those in Renal Extract

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