Effect of Actidione, Griseofulvin and Triphenyltin Acetate on the Kinetics of Fungal Growth

Trinci, Anthony P. J.; Gull, Keith

doi:10.1099/00221287-60-3-287

Cited by 23 publications

(3 citation statements)

References 6 publications

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“…The plates were incubated at 25 "C for 3 d (for viable counts) or 6-8 d (to detect cycloheximide resistance). In filamentous fungi, the time (lag phase) between inoculation and germination (and, hence, colony expansion) is altered by cycloheximide (Hsu, 1963;Trinci & Gull, 1970;Pongratz & Klingmiiller, 1973) and therefore the incubation period was extended appropriately.…”

Section: Chemostat Culturesmentioning

confidence: 99%

Periodic selection in longterm continuous-flow cultures of the filamentous fungus Fusarium graminearum

Wiebe

Robson²,

Cunliffe³

et al. 1993

Journal of General Microbiology

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By monitoring increases and decreases in the proportion of cycloheximide-resistant macroconidia, periodic selection was observed in populations of the filamentous fungus Fusarium graminearurn, grown in glucose-limited chemostat cultures. The results indicated that periodic selection of advantageous mutants of F. graminearurn occurred at intervals of about 124 h at both high (D = 0.19 h-', approximately 34 generations) and low (D = 0-06 h-', approximately 11 generations) dilution rates. Several 'adaptive' peaks (each indicating the appearance of an advantageous mutation) were observed before morphological (highly branched) mutants appeared in the populations; these mutants have previously been observed to have a selective advantage over the parental strain. At intervals, macroconidia harvested from the chemostat were used to inoculate plates of non-antibioticcontaining agar medium, and it was possible to monitor periodic selection in the original chemostat culture using second generation macroconidia harvested from these cultures. The proportion of cycloheximide-, potassium chlorate-, and p-fluoro-DL-phenylalanine-resistant macroconidia in these second generation macroconidia changed in a pattern similar to that observed when monitoring the proportion of cycloheximide-resistant macroconidia in the first generation population harvested directly from the chemostat. The experiments demonstrated that populations of filamentous fungi are heterogeneous and that much of this heterogeneity may already be present at the end of batch growth, i.e. before the onset of continuous cultivation.

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Section: Chemostat Culturesmentioning

confidence: 99%

Periodic selection in longterm continuous-flow cultures of the filamentous fungus Fusarium graminearum

Wiebe

Robson²,

Cunliffe³

et al. 1993

Journal of General Microbiology

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“…Cultures were incubated at 25°C for 7 days before measuring colony diameter along two perpendicular axes. Growth rate (mm/d) was calculated as: K r = ( d 1 – d 0 ) / ( t 1 – t 0 ) (Trinci & Gull, 1970), where d 1 and d 0 are radial colony diameter at initial ( t 0 ) and elapsed times ( t 1 ). The colour and shape of the colonies were recorded visually.…”

Section: Methodsmentioning

confidence: 99%

Identification of Corynespora cassiicola causing leaf spot on Syringa species

Wang

Liu

2021

Forest Pathology

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Lilacs (Syringa spp.), a genus of trees and shrubs in the Oleaceae, are known for their beautiful, fragrant flowers and are widely cultivated as ornamentals. In September 2017, a new leaf spot disease was observed on S. oblata in the Sui and Tang Ruins Dynasties Botanical Garden in Luoyang, Henan Province, China. Leaf symptoms appeared as small brown lesions at earlier stages, which with age turned into suborbicular brown, faintly zonate and depressed. Fully mature lesions were mostly irregular to circular, concentric rings with taupe and dark brown bands. A total of 12 monoconidial isolates were obtained from S. oblata showing leaf symptoms. Colonies were grey to light brown and darker underneath on potato dextrose agar. Based upon morphological characteristics and multilocus phylogenetic analyses using ITS, TEF1 and RPB2 loci, isolates were identified as Corynespora cassiicola. Pathogenicity tests on leaves produced symptoms matching those described above and the fungus was re‐isolated, confirming Koch's postulates. This study represents the first report of C. cassiicola on lilacs in China.

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“…Initially the growth tube method of Ryan et al (1943) was used, but for the majority of the observations reported later radial growth rates were estimated on agar media in Petri dishes, this method being most convenient for handling large numbers of estimations simultaneously and being particularly suitable for studying the effects of inhibitors on fungal growth rates (Trinci and Gull 1970). Careful control of conditions was necessary in order to obtain the desired degree of reproducibility and, in particular, variation of agar depth was found to influence growth rate considerably, as previously reported by Leonian (1934).…”

Section: (B) Determination 0/ Growth Rates 0/ Isolatesmentioning

confidence: 99%

Separation of Two Ecotypes of Phytophthora Drechsleri Tucker Occurring in Australian Native Forests

Shepherd¹,

Pratt²

1973

Aust. Jnl. Of Bio. Sci.

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A total of 70 isolates of P. drechsleri from Australian native eucalypt forests were separated into two distinct ecotypes. The "northern" ecotype occurred from North Queensland to the south of New South Wales, while the "southern" ecotype occurred in South Australia, Victoria, Tasmania, and New South Wales.The northern ecotype on average grew faster on a variety of media than did the southern one, but ecotypes could not be recognized unequivocally on this basis. The upper temperature limit for growth of the northern ecotype was in the range 36-37' 5°C, while that for the southern was 33-36°C. Ecotypes could be separated on the bases of their growth rates in the presence of 33 .ug/ml Cu2+ ions (23' 54 and 55· 66 % of the growth in the absence of copper by the northern and southern ecotypes respectively), orin the presence of 1 p.p.m. pyronin G (63' 34 and 33· 87 % of the growth in the absence of pyronin G by the northern and southern ecotypes respectively). Both ecotypes showed similar pH optima for growth, similar responses to the effects of exposure at 44°C on subsequent growth at 25°C, and similar degrees of growth inhibition by 0·05 p.p.m. malachite green.It is tentatively suggested that the geographic distribution of the southern ecotype is related to the area enclosed by the 29·4 °C (85°F) isotherm of average daily maximum temperature during the period November to March annually.

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Effect of Actidione, Griseofulvin and Triphenyltin Acetate on the Kinetics of Fungal Growth

Cited by 23 publications

References 6 publications

Periodic selection in longterm continuous-flow cultures of the filamentous fungus Fusarium graminearum

Periodic selection in longterm continuous-flow cultures of the filamentous fungus Fusarium graminearum

Identification of Corynespora cassiicola causing leaf spot on Syringa species

Separation of Two Ecotypes of Phytophthora Drechsleri Tucker Occurring in Australian Native Forests

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