Background: Most dental anxiety is caused by the pain of local anesthetic injection. Techniques such as acupressure have been introduced to reduce anxiety and pain. This study aimed to investigate the effect of acupressure on the reduction of anxiety and pain during local anesthetic injection in children 5–7 years old. Methods: This triple-blinded clinical trial was conducted on 71 children between the ages of 5 and 7. The children were divided into two groups, and acupressure was performed at key points (Yintang and Hegu) in one group and placebo points in the second group before local anesthesia. Then, the level of anxiety and pain was measured. The tools used to measure dental anxiety and injection pain were the visual analog scale (VAS-A) and FPS-R (Facial Pain Scale-Revised), respectively. Data were analyzed using SPSS version 20 software by analysis of variance and Wilcoxon test at the significance level of 0.05. Results: The results of the present study showed that acupressure was significantly effective in reducing dental anxiety and injection pain in children (P<0.001). Also, the results showed that the acupressure technique significantly reduced pain in 5- and 6-year-old children but not in 7-year-old children (P value=0.22). Our results indicated that acupressure at the key points significantly reduced anxiety and pain in both genders (P value<0.05). However, the effect was not significant after applying pressure to placebo points. Conclusion: Based on the results of the present study, the acupressure technique is significantly effective in reducing anxiety in children 5–7 years old and injection pain in 5- and 6-year-old children in both genders.