Effect of Acute Stress on the Pituitary Gland: Uptake of Serine-1-C14Into ACTH

Jacobowitz, David M.; Marks, Bernard H.; Vernikos‐Danellis, Joan

doi:10.1210/endo-72-4-592

Cited by 24 publications

(16 citation statements)

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“…Vernikos-Danellis [1963] demonstrated that the stress-induced rise of pituitary ACTH occurs more readily in chronically adrenalectomized rats and is also sensitive to cortisol in hibition, but the method of determining total gland content of ACTH did not show such cortisol sensitivity in non-stressed animals. These S r AC K i iouse/N ov ai./L eathem findings, together with our own, suggest the possibility that the slowly developing increased rate of synthesis in adrenalectomizcd rats is under the control of a mechanism that is at least in part different from the normal mechanism in intact rats and it maintains a consid erably higher basal rate of synthesis.…”

Section: Discussionmentioning

confidence: 97%

Effect of Adrenalectomy and Cortisol on the in vivo Rate of Incorporation of Phenylalanine-14C into Rat Corticotropin

Stackhouse¹,

Noval²,

Leathem³

1971

Neuroendocrinology

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A method has been developed for studying the rate of in vivo ACTH synthesis by measuring the incorporation of phenylalanine-¹⁴C into ACTH in the rat. At 1 h after the intra-peritoneal injection of labeled amino acid the anterior pituitary was obtained and ACTH was extracted with hot glacial acetic acid, precipitated with ether, and adsorbed onto oxidized cellulose. The ACTH fraction was then eluted with 0.1 N hydrochloric acid. This fraction was shown to contain radioactive ACTH and did not contain free phenylalanine-¹⁴C. Changes in the rate of ACTH synthesis were estimated by changes in specific activity of the ACTH following a 1 h exposure of the rat to isotope. At 7, 14, and 24 days following adrenalectomy the rate of pituitary ACTH labeling doubled, while at 40 days, there was a three- to fivefold increase over the control rate. Administration of cortisol to the adrenalectomized but not to the intact rat decreased the rate of pituitary ACTH labeling. Nearly total inhibition of ACTH synthesis was caused by cortisol (0.5 mg/100 g body wt.) within 2 h. It was found that changes in the rate of incorporation of labeled amino acids into ACTH were not a reflection of general changes in the rate of tissue protein synthesis.

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Section: Discussionmentioning

confidence: 97%

Effect of Adrenalectomy and Cortisol on the in vivo Rate of Incorporation of Phenylalanine-14C into Rat Corticotropin

Stackhouse¹,

Noval²,

Leathem³

1971

Neuroendocrinology

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show abstract

“…However Vernikos Danellis demonstrated that a single blocking dose of hydrocortisone prevented both release and increase in pituitary ACTH contents after stress but had no effect on the resting level in the blood and gland (25). The facts therefore appear to be that the synthesis and secretion of ACTH in the gland occur differently in resting stage and in response to stress, and that the corticoid inhibit the stress-induced ACTH release, not by depressing pituitary ACTH stores but by interfering with ability of the gland to synthesize new ACTH (26)(27)(28)(29)(30). Further studies are needed to clarify the mechanism by which CCI, inhibits the additional hypophyseo-adrenal activation.…”

Section: Discussionmentioning

confidence: 95%

The Effect of Carbon Tetrachloride on Plasma Corticosterone Level

Furukawa¹

1966

Japanese Journal of Pharmacology

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“…Obviously, these noticeable changes are intimately related to the overproduction of gonadotrophic hormones following castration. In fetal rat, however, basophilic cells appeared to be enlarged in less Vol.19 (Siperstein, 1963;Jacobowitz et al, 1963;Wakabayashi and Tamaoki, 1965;Wakabayashi et al, 1968).…”

Section: Discussionmentioning

confidence: 99%