Effect of Adding Resveratrol to Soybean-Lecithin Extender on Some Semen Attributes of Buffalo Bulls

This study was undertaken to identify some protein profiles in the seminal plasma of Iraqi buffalo bulls and their correlation to the quality of fresh and cryopreserved semen. Semen was collected from eight buffalo bulls and divided according to the percentage of sperm individual motility into two groups. The first group (n=3) included good semen quality, while the second group (n=5) included poor semen quality. Seminal plasma was extracted from each semen sample by centrifugation and seminal proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Nine types of protein bands were observed in the seminal plasma of the buffalo bulls belonging to good semen quality, ranging from 10-68 kDa. The most abundant proteins in the overall presence of proteins in seminal plasma were 10 (100%), 14 (100%), 20 (100%), 26 (100%), 34 (100%), and 43 (37.5%) kDa. On the other hand, 14 types of protein bands resolved for the poor-quality semen, ranging from 10-155 kDa. The most abundant proteins in the overall presence of proteins in seminal plasma were 10 (100%), 14 (100%), 20 (100%), 26 (100%), 34 (87.5%), 130 (50%) and 155 (50%) kDa. A significant negative correlation was noticed among proteins with molecular weights of 28. 88 ± 3.26, 22.50 ± 2.85, and 12.00 ± 1.15 kDa, and the percentage of sperm individual motility and plasma membrane integrity. Moreover, there was a positive significant correlation between proteins whose molecular weight 22.50 ± 2.85 kDa and the percentages of total abnormalities and normal sperms. In conclusion, the identification of seminal proteins may be a useful tool in the future evaluation of spermatozoa and the prediction of fertility in Iraqi buffalo bulls.

Section: Fresh Semenmentioning

confidence: 99%

Protein Profiles in Seminal Plasma of Iraqi Buffalo Bulls (Bubalus bubalis) Associated with Fresh and Cryopreserved Semen Quality

Musa,

Abdulkareem

2023

“…Sperm cryopreservation has contributed to reducing the obstacles and factors that make it hard to obtain semen, such as the geographical location and the time factor [4]. The semen of buffalo bulls is high in unsaturated fatty acids and contains a few antioxidants [5,6] compared to cow bulls [7,8]. Therefore, the semen of buffaloes is more susceptible to lipid oxidative damage by reactive oxygen species (ROS), which leads to an increase in the percentage of abnormalities, damage to the genetic material, and a decrease in fertility [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…The semen of buffalo bulls is high in unsaturated fatty acids and contains a few antioxidants [5,6] compared to the Holstein bulls [7,8,9]. Therefore, the semen of buffaloes is more susceptible to lipid oxidative damage by free radicles of reactive oxygen species (ROS), which leads to an increase in the percentage of abnormalities, damage to the genetic material, and a decrease in fertility [10,11]. In semen, free radicles or ROS are generated by leukocytes, dead, abnormal, and immature spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

“…Buffalo bulls are characterized by a lack of sperm concentration, motility, and viability, a weak sperm mitochondrial membrane, and maturation within the epididymis [12]. The effects of free radicals or ROS on the semen of buffalo bulls appear during spermatogenesis and cryopreservation [6,11,13,14]. The major obstacle facing antioxidant addition to the buffalo bull's semen extender is the failure to treat the affected sperm with reactive oxygen species during the spermatogenesis process in the testes, so there is an urgent need to give antioxidants by treating the animals directly [15].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Melatonin Implantation on Sexual Behavior and some of Semen Quality of Iraqi Buffalo Bulls

Nsaif,

Eidan

2023

This study investigated the effect of melatonin implantation on sexual behavior and some aspects of the semen quality of buffalo bulls. Nine buffalo bulls had divided into three groups (3/group). The first group was the control group (C1). The buffalo bulls were implanted with melatonin hormone in the second (72 mg; C2) and third (90 mg; T3) groups under the skin of the left ear base and repeated one month after the first implantation. Semen was collected for 14 weeks and evaluated weekly. The first amount of time was lower in the C3 group than in the C2 and C1 groups. Semen volume at 2nd ejaculation was higher (P<0.05) in the C3 group than in other groups. Mass activity and motility increased in the C3 group compared to the C2 and C1 groups at different ejaculation times. The melatonin implantation (90 mg) can improve the semen quality of Iraqi bulls which in turn enhancing the reproductive performance, conception rate of female buffalo and increasing the artificial insemination productivity and owner’s income.

“…Semen is affected by many factors, including genetics, age, stress, nutrition, management, diseases, care, transportation, vaccination, and others [3,9,10,11,12,13]. There is a strong relationship between male fertility and semen characteristics fresh of buffalo bulls [14,15,16] and bovine bulls [17,18]. The sperm separation techniques significantly improved semen quality (good sperm with progressive motility and normal morphology) in in vitro fertilization (IVF).…”

Section: Introductionmentioning

confidence: 99%

Effect of Glass Wool and Sephadex Sperm Separation Techniques on Improving the Poor Quality Semen of Iraqi Buffalo Bulls

Alwaeli,

Eidan

2023

This study investigated the effect of glass wool and Sephadex separation techniques on improving the poor semen quality of buffalo bulls. Semen had divided into four groups. The first and second groups used good (G1, positive control) and poor-quality semen (G2, negative control). Glass wool (G3) and Sephadex (G4) techniques had used to separate the poor semen quality in the 3rd-4th groups, respectively. The sperm concentration and total abnormalities were lower in the G3 and G4 groups than in the G2 group. Motility increased in the G4 group compared to the G2 group. Live sperm, normal morphology, plasma membrane, and acrosome integrity were higher in groups G4 and G3 than in the G2 group. In conclusion, after separation, there was a decrease in sperm concentration compared to non-separation. This decrease had compensated by a proportionate increase in other semen characteristics, which may improve the conception rate via artificial insemination with filtered semen.