Effect of addition of Ca²⁺ to titanium by a hydrothermal method on soft tissue sealing

Abstract

The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca 2+ ) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectroscopy; proliferation of hGF-1 cells was measured by the CCK-8 assay; immunofluorescence staining was done to detect adherent proteins on titanium surface. In vivo, the degree of attachment between the implant and the surrounding soft tissue was measured by horseradish peroxidase (HRP). The percentage of hGF-1 cells adhering in the Ca group was significantly higher (p < .01); the fluorescence of integrin-β1 and F-actin in the Ca group was stronger; Ca group had the shorter length of HRP (p < .01). Ca 2+ can be added to the surface of titanium by a hydrothermal method and it will be more beneficial for soft tissue early sealing.

Surface Modification and Functionalities for Titanium Dental Implants

“…The Ti surface is covered with another material, which can change the composition of the implant surface to a greater extent and achieve more excellent biomedical functions. Coating technologies include plasma spraying, , nanospray drying, sol–gel method, , hydrothermal method, , self-assembly method, , 3D printing, and so on.…”