Effect of Addition of Melatonin on the Liquid Storage (5°C) of Mithun (<i>Bos frontalis</i>) Semen

Perumal, P.; Vüpru, Kezhavituo; Khate, K.

doi:10.1155/2013/642632

Cited by 43 publications

(39 citation statements)

References 58 publications

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“…The HOST results in the present study are in conformation with the studies of Ashrafi et al (), El‐Raey, Badr, Assi, & Rawash () in bulls; Perumal et al (), Perumal et al () in Mithun; Chankitisakul () in boars at 15˚C and Izadpanah et al () in stallions; in which plasma membrane integrity improved with the addition of melatonin.…”

Section: Discussionsupporting

confidence: 92%

“…The results of acrosomal integrity in melatonin treated semen are similar to those reported by Cheuqueman et al (), Kapadiya et al (), Pang et al () in bulls; Perumal et al (), Perumal et al () in Mithun; El‐Raey, Badr, Rawash, and Darwish () in goats and Martin‐Hidalgo et al () in boars. The present study is in conclusion of melatonin capacity in shielding acrosomal characters by keeping check on hazardous effects encountered during cryopreservation.…”

Section: Discussionsupporting

confidence: 88%

“…The data obtained regarding sperm motility are in accordance with Ashrafi et al, (), Cheuqueman et al, (), Abdel‐Khalek, El‐Nagar, and Omima (), Kapadiya, Nakhashi, Sutaria, Rathod, and Suthar (), Pang et al, (), Asma‐ul‐Husna et al, () in bulls; Perumal, Vupru, and Khate (), Perumal et al, (), Perumal, Chang, Sangma, Savino, and Khate (), Perumal, Baruah, et al () in Mithun at 5°C; Ashrafi, Kohram, Naijian, Bahreini, and Poorhamdollah (), Succu et al, (), Casao et al, () in rams; Balao da Silva et al, () in stallions at 37°C; Izadpanah, Zare‐Shahneh, Zhandi, Yousefian, and Emamverdi () in stallions at 5°C and El‐Battawy and El‐Nattat () in goats; who reported that the addition of melatonin to semen freezing extender could protect the spermatozoa from damage during cryopreservation and improve the post‐thaw semen quality.…”

Section: Discussionsupporting

confidence: 85%

See 2 more Smart Citations

Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation

ChaithraShree¹,

Ingole

Dighe

et al. 2019

Veterinary Medicine & Sci

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The production of reactive oxygen species (ROS) during cryopreservation of semen alters the sperm motion and mobility characteristics, resulting in poor or failure of conception rate after artificial insemination (AI). Melatonin an antioxidant is able to modulate the effect of ROS and prevents spermatozoa by reducing the oxidative stress during freezing process. Eight ejaculates from eight healthy HF bulls diluted with Tris egg yolk glycerol extender were divided into five equal aliquots. The Computer Assisted Semen Analyser (CASA) results showed no significant difference between the control—post‐ thaw samples and melatonin‐treated samples; however, the velocity of spermatozoa with regard to the VAP, VSL showed highest increase in the 0.25 mM MLT‐treated semen followed by 0.1 mM MLT treated semen except for VCL where velocity increased with increase in the concentration of melatonin. The vigour of spermatozoa regard to BCF, STR and LIN recorded highest increase in the 0.25 mM MLT treated semen followed by 0.1 mM MLT‐treated semen except for the ALH where vigour increased with increase in the concentration of melatonin. The electron micrography images illustrated that the addition of 0.1 mM melatonin protected the plasma membrane and acrosome region and maintained the ultrastructure integrity of the cryopreserved spermatozoa when compared to control group, whereas the electron micrography of spermatozoa treated with 0.2 and 0.25 mM melatonin illustrated highest damage to the plasma and acrosome membrane. Thus concluding that inclusion of melatonin to sperm extender can improve the post‐thaw quality of cryopreserved bull spermatozoa.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 85%

See 1 more Smart Citation

Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation

ChaithraShree¹,

Ingole

Dighe

et al. 2019

Veterinary Medicine & Sci

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“…It was also reported that melatonin minimizes or prevents the in vitro sperm capacitation and inhibit apoptotic like changes which indicates it has a direct action on spermatozoa. Inclusion of melatonin into the ram sperm (Ashrafi et al, 2011), boar sperm (Hyun-Yong et al, 2006), bull sperm (Ashrafi et al, 2013) and mithun (Perumal et al, 2013d) has been reported that it protected the sperm against the harmful effects of free radicals or ROS and improved the sperm motility, membrane integrity and ferility in the frozen state.…”

Section: Introductionmentioning

confidence: 99%

Melatonin in Semen Extender Improves Freezability in Mithun

Perumal¹

2019

IJBSM

Self Cite

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Melatonin plays important roles in circadian clock regulation and seasonal reproduction in mammals. It is also a potential antioxidant and anti-apoptotic agent in semen preservation. Season/climatic factors/photoperiod and temperature humidity index is one of the most important factors, effect on the variation in semen quality parameters (SQPs) and fertility rate. The present study was conducted to assess the effect of melatonin (MT) on SQPs at different seasons of the year such as sperm motility, viability, total sperm abnormality, intactness of acrosome, functional plasma membrane and nuclear integrity in mithun. A total numbers of 80 ejaculates (20 ejaculates in each season) were collected twice per week from mithun bulls by transrectal massage method and semen sample was divided into six equal aliquots and diluted with the Tris egg yolk citrate glycerol extender (TEYCGE). Group 1: semen without additives (control), group 2 to 6: semen was diluted with 1, 2, 3, 4 and 5 mM of melatonin, respectively. These SQPs were estimated in frozen thawed semen. Inclusion of melatonin into the diluent resulted in significantly (p<0.05) decreased dead and abnormal spermatozoa percentage and acrosomal abnormalities whereas significant improvement was observed in the sperm motility, viability, plasma membrane and nuclear integrity in treatment as compared to control group at different seasons of the year. The present study result clearly indicated that melatonin protected the post thaw SQPs in different seasons and also semen quality varied from different seasons and was significantly higher in spring and lowest was in summer season.

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“…Using semen cryopreserved in extender containing hen's egg yolk (EY), Mondal, Karunakaran, Lee, and Rajkhowa () inseminated 16 mithun cows and achieved 12 pregnancies. When reduced glutathione as an antioxidant was added in the extender, improvement in quality of mithun semen was observed (Perumal, Vupru, & Rajkhowa, ). Although researchers have used diluters of varied composition to limit the cellular damages, the EY remains a most common component.…”

Section: Introductionmentioning

confidence: 99%

Effects of low‐density lipoproteins as additive on quality parameters and oxidative stress following cryopreservation of mithun (Bos frontalis) spermatozoa

Perumal

Srivastava

Ghosh

et al. 2016

Reprod Domestic Animals

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Artificial breeding of mithun poses several challenges including lack of standard protocol for cryopreservation of spermatozoa. This is further complicated by harmful effects of hen's egg yolk (EY) as additive in extender. Purified low-density lipoproteins (LDL) extracted from EY have been shown as beneficial over EY extender for long-term semen storage in several species. This investigation explored use of LDL versus EY on semen quality and oxidative stress following freezing-thawing of spermatozoa. A total of 25 of 50 ejaculates based on biophysical parameters were selected for the experiment. After diluting with the Tris-citrate-glycerol (TCG) extender, each sample was split into three equal aliquots: Group I, control, EY; Group II and Group III contained 8% and 10% purified LDL, respectively. Frozen-thawed samples were evaluated for motility parameters (progressive, and in the bovine cervical mucus penetration test [BCMPT]), viability, sperm and nuclear abnormality, acrosome integrity, and enzymatic (leakage of intracellular contents) and biochemical (oxidative stress) profiles and in vitro fertility (IVF) assay. Study revealed a significant (p < .05) improvement in viability, sperm and nuclear abnormality, acrosome integrity, motility (progressive and in cervical mucus), cholesterol content, and reduction in the leakage of intracellular enzymes in Group II. Moreover, intactness of acrosome and biochemical membranes was protected significantly (p < .05) in addition to significant (p < .05) improvement in binding per cent and binding index in IVF assay in extender containing 8% LDL. These results demonstrate that although cryopreservation of mithun's spermatozoa in EY was comparable with other species, addition of 8% LDL holds a clear advantage over EY or 10% LDL.

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Effect of Addition of Melatonin on the Liquid Storage (5°C) of Mithun (Bos frontalis) Semen

Cited by 43 publications

References 58 publications

Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation

Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation

Melatonin in Semen Extender Improves Freezability in Mithun

Effects of low‐density lipoproteins as additive on quality parameters and oxidative stress following cryopreservation of mithun (Bos frontalis) spermatozoa

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