Effect of adenine sulphate, casein hydrolysate and spermidine on in vitro shoot multiplication of two banana varieties (FHIA-21 and PITA-3)

Silue, Oumar; Kouassi, Kan Modeste; Koffi, Kouablan Edmond; Kouakou, Konan Eugene Pacome; Aké, S.

doi:10.5897/ajb2017.16247

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Previous studies revealed the positive impact of growth additives in the regeneration frequency of several plant species. For instance, the use of 25 to 50 mg/l of casein hydrolysate in combination with 4 mg/l BAP significantly improved shoot regeneration in bananas [16]. Similarly, the addition of 500 mg/l casein hydrolysate into the MS medium having 1 μM BAP induced the maximum shoot number in Neem tree [17].…”

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confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

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Section: Introductionmentioning

confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Optimización en la aclimatación de plántulas micropropagadas de banano (Musa sp.) utilizando tres insumos orgánicos.

Mora-González

Naranjo-Morán

Albiño-Quitiaquez

et al. 2021

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El éxito de la micropropagación de plantas in vitro depende en parte de la fase de aclimatación, esta etapa presenta problemas de supervivencia y conlleva extensas semanas de adaptación. En este sentido, la utilización de microorganismos eficientes nativos son una alternativa biotecnológica para adaptar plantas in vitro. El presente trabajo tiene como objetivo evaluar el efecto de la aplicación de Trichoderma ghanense, micorrizas arbusculares y un biofertilizante líquido en plantas meristemáticas de banano variedad William en las fases de aclimatación y vivero. Para los ensayos en las fases de aclimatación y vivero se realizaron 20 tratamientos y se utilizó un diseño factorial 2(k) con una duración de seis semanas en ambas fases. Los resultados demuestran que en la fase de aclimatación el mejor tratamiento fue el B10 con incrementos del 29,6 % de altura y 19,9 % de diámetro respecto al control; mientras que, para el área foliar fue el B19 con 84,7 % de incremento en comparación al control. En fase de vivero el mejor tratamiento fue el B19 presentando incrementos del 14,5 % altura, 19,3 % diámetro del pseudotallo, 13,4 % área foliar, 91,8 % longitud radicular, 39,98 % peso húmedo y 90,5 % peso seco en comparación al control. Ambas fases alcanzaron porcentaje de micorrización mayores al 45 %. Los porcentajes de supervivencia fueron del 100 % en fase de aclimatación. Por lo expuesto, se concluye que los insumos orgánicos constituyen una alternativa para el manejo y adaptación de plantas producidas in vitro.

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Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review

Agbadje

Agbidinoukoun

Zandjanakou-Tachin³

et al. 2021

EJBIO

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Bananas and plantains are among the most important food crops in Central and West Africa. Their plantation is lead to many problems. In the recent decades, biotechnology tools using in vitro culture technics are used for the mass and free disease plantlets production in order to increase the bananas production and the yield. The main way of in vitro tissue culture at this end is the direct organogenesis i.e., the ability of plant tissues to form various organs de novo by shoots or roots induction to differentiate from a cell or cell clusters. This review aims to summarize the main results obtained in the organogenesis of bananas and plantains (Musa spp.) under in vitro conditions and to identify the challenges during the process. The research articles used in this review show that micropropagation is a reliable alternative to conventional production system of bananas and plantains planting material. However, the use of the in vitro micropropagation for bananas and plantains entails choosing the optimal explant type and size according to objectives. Benzylaminopurine remains the preferred cytokinin for in vitro banana and plantain shoot proliferation, while the use of thidiazuron appears to be more and more common. Whichever cytokinin used, the optimal cytokinin concentration for shoot proliferation is genotype dependent. This review also focuses on the causes and control measures of the two major banana and plantain micropropagation constraints: lethal tissues browning/darkening and microbial contaminations. It showed that applying the suitable and available control measure, according to the evolution of culture, is necessary. All this available information on the in vitro conditions makes banana and plantain cultivars in vitro organogenesis possible.

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Effect of adenine sulphate, casein hydrolysate and spermidine on in vitro shoot multiplication of two banana varieties (FHIA-21 and PITA-3)

Cited by 3 publications

References 17 publications

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Optimización en la aclimatación de plántulas micropropagadas de banano (Musa sp.) utilizando tres insumos orgánicos.

Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review

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