Effect of Adipose‐Derived Stem Cells on Head and Neck Squamous Cell Carcinoma

Danan, Deepa; Lehman, Christine E.; Mendez, Rolando E.; Langford, B.; Koors, Paul D; Dougherty, Michael I.; Peirce, Shayn M.; Gioeli, Daniel; Jameson, Mark J.

doi:10.1177/0194599817750361

Cited by 10 publications

(12 citation statements)

References 17 publications

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“…For augmentation of volume defects and treatment of impaired wound healing, adipose tissue transplantation is an established regenerative and reconstructive approach in head and neck cancer patients [ 48 ]. Therefore, ASCs are injected into tissues that might still contain residual tumor cells; in the head and neck area, these are most likely from squamous cell carcinoma [ 35 , 49 ]. In a literature search using the search terms “ASC AND HNSCC”, “adipose stem cells AND HNSCC”, “MSC AND HNSCC” and “mesenchymal stem cells AND HNSCC”, we found only six studies that examined the role of ASCs on HNSCC progression [ 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…To date, only eight studies have linked MSCs/ASCs and HNSCC [ 24 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. Grønhøj et al evaluated the salivary gland function after ASC injection in patients with radiation-induced xerostomia after HPV-related oropharyngeal squamous cell carcinoma, but oncological safety was not an endpoint [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Chiu et al tested paracrine effects of ASC-conditioned medium on oral squamous cell carcinoma and found that it might protect cancer cells from cisplatin-induced cell death but did not find alterations in tumor cell growth and migration [ 34 ]. In different in vitro approaches and an in vivo xenograft model, Danan et al found that ASCs did not significantly stimulate HNSCC cell proliferation or migration, and only increased survival in one single cancer cell line tested [ 35 ]. Rowan et al did not find alterations in growth of HNSCC cells in vitro or tumor xenografts ex vivo, but found stimulation of cancer cell migration, integration of ASCs to tumor–stroma, and early micrometastasis in vivo [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

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Human Adipose-Derived Stem/Stromal Cells Promote Proliferation and Migration in Head and Neck Cancer Cells

Sharaf

Eggersmann

Haider

et al. 2021

Cancers

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Human adipose-derived stem/stromal cells (ASCs) are increasingly used as auto-transplants in regenerative medicine to restore tissue defects or induce wound healing, especially in cancer patients. The impact of ASCs on squamous cell carcinoma of the upper aerodigestive tract (UAT) including head and neck and esophageal squamous cell carcinoma (HNSCC and ESCC) is not yet fully understood. ASCs were cultured from subcutaneous, abdominal lipoaspirates of five patients, who received auto-transplants to the head and neck. Supernatants were tested for paracrine effects in functional in vitro assays of proliferation of HNSCC tumor cell line FaDu and ESCC cell line Kyse30, and their cell migration/invasion capacities in Boyden chambers, in addition to endothelial tube formation assay using human umbilical vein endothelial cells (HUVECs). All ASC-derived supernatants enhanced proliferation of FaDu cells, invasive migration, and tube formation by HUVECs, compared to controls. Of five patients’ lipoaspirates, ASC-derived supernatants of four patients increased proliferation and invasive migration in Kyse30 cells. The data suggests that ASCs can promote tumor cell proliferation, invasiveness, and neo-angiogenesis in these tumor cell lines of the UAT and HUVEC in a paracrine manner. Although clinical studies on the subject of oncological safety are still needed, these findings emphasize the importance of complete tumor removal before ASCs are used in the head and neck.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human Adipose-Derived Stem/Stromal Cells Promote Proliferation and Migration in Head and Neck Cancer Cells

Sharaf

Eggersmann

Haider

et al. 2021

Cancers

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“…In our previous studies, it was shown that ADSCs promote the proliferation and invasion of ovarian cancer cells and play a role in the formation of omental premetastatic niches in ovarian cancer [6]. Increasing evidence supports that ADSCs regulate many changes in the TME during tumour progression by secreting soluble factors and extracellular vesicles [10–14]. However, we know little about the exact mechanisms by which ADSCs promote ovarian cancer cells' growth and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression

Chu

You

Zhang

et al. 2019

Stem Cells International

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As shown in our previous studies, growth and metastasis of ovarian cancer can be regulated by adipose-derived mesenchymal stem cells (ADSCs). However, the underlying mechanism has not yet been revealed. In this study, a proteomics analysis was performed to compare protein expression treated with and without ADSCs in ovarian cancer cells. Protein levels were altered in ovarian cancer cells due to the treatment of ADSCs. Thymosin beta 4 X-linked (TMSB4X) levels changed dramatically, and this protein was identified as one of the most important candidate molecules contributing to the tumour-promoting effects of ADSCs. Compared with the cells that are cultured in the normal growth medium, the TMSB4X levels cultured in ADSC-conditioned medium increased significantly in ovarian cancer cells. Furthermore, the growth and invasion of cancer cells were decreased, even in the ADSC-conditioned medium treatment group (P < 0.05), by the inhibition of TMSB4X. As shown in the bioluminescence images captured in vivo, increased ovarian cancer's growth and metastasis, along with elevated TMSB4X expression, were observed in the group of ADSC-conditioned medium, and the tumour-promoting effect of ADSCs was attenuated by the inhibition of TMSB4X. Based on our findings, increased TMSB4X expression may play a role in accelerating the ADSC-mediated proliferation, invasion, and migration of ovarian cancers.

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“…Some concerns have been raised about the possibility of administered fat to promote residual tumor cell invasion and metastasis [80]. Preliminary data obtained using head and neck cancer cell lines both in vitro and in vivo suggest that the procedure may be safe, but further investigation performed on patientderived tumor samples is needed [81].…”

Section: Head and Neck Reconstruction After Radiotherapymentioning

confidence: 99%

Regenerative medicine approaches for the management of respiratory tract fistulas

et al. 2020

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Respiratory tract fistulas (or fistulae) are abnormal communications between the respiratory system and the digestive tract or the adjacent organs. The origin can be congenital or, more frequently, iatrogenic and the clinical presentation is heterogeneous. Respiratory tract fistulas can lead to severely reduced health-related quality of life and short survival. Therapy mainly relies on endoscopic surgical interventions but patients often require prolonged hospitalization and may develop complications. Therefore, more conservative regenerative medicine approaches, mainly based on lipotransfer, have also been investigated. Adipose tissue can be delivered either as unprocessed tissue, or after enzymatic treatment to derive the cellular stromal vascular fraction. In the current narrative review, we provide an overview of the main tissue/cell-based clinical studies for the management of various types of respiratory tract fistulas or injuries. Clinical experience is limited, as most of the studies were performed on a small number of patients. Albeit a conclusive proof of efficacy cannot be drawn, the reviewed studies suggest that grafting of adipose tissue-derived material may represent a minimally invasive and conservative treatment option, alternative to more aggressive surgical procedures. Knowledge on safety and tolerability acquired in prior studies can lead to the design of future, larger trials that may exploit innovative procedures for tissue processing to further improve the clinical outcome.

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Effect of Adipose‐Derived Stem Cells on Head and Neck Squamous Cell Carcinoma

Cited by 10 publications

References 17 publications

Human Adipose-Derived Stem/Stromal Cells Promote Proliferation and Migration in Head and Neck Cancer Cells

Human Adipose-Derived Stem/Stromal Cells Promote Proliferation and Migration in Head and Neck Cancer Cells

Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression

Regenerative medicine approaches for the management of respiratory tract fistulas

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