Effect of adlay (Coix lachryma‐jobi L. var. ma‐yuen Stapf.) hull extracts on testosterone release from rat Leydig cells

Abstract

Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3',5'-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17beta-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

Phytochemical screening, antibacterial and anthelmintic activities of leaf and seed extracts of Coix lacryma-jobi L.

“…Job's tears are taken by people due to its beneficial effect for hay fever, high cholesterol, cancer, warts, obesity, arthritis, allergic disorder and respiratory tract infections. Many scholars have reported different biological activities of Job's tears, including antioxidant/free radical scavenging [12], anti-inflammatory [13,14], anti-tumor [12,15,16], hypolipidemic [17], hypocholesterolemic [18], anti-allergic [19,20], hypoglycemic [21], antiobesity [22], anti-mutagenic [23], anti-ulcer [24], prebiotic activity [25], hormonal modulation [26], osteoporosis preventing [27], abortifacient [28] and antimicrobial effect [29].…”

Potential benefits of adlay on hyperandrogenism in human chorionic gonadotropin-treated theca cells and a rodent model of polycystic ovary syndrome

“…These effects on steroidogenesis may be mediated through the cAMP-PKA pathway. AHE inhibits progesterone secretion by ovarian GCs (Hsia et al, 2007) and inhibits testosterone secretion by Leydig cells (Hsia et al, 2009). Kao et al (Kao, Zhou, Sherman, Laughton, & Chen, 1998) reported that the structure of flavonoids such as chrysin and genistein is similar to that of androgens; thus, flavonoids may interact with the androgen receptor through competitive inhibition.…”

“…Ovulationinducing substances were isolated from adlay extracts (Kondo, Nakajima, Nozoe, & Suzuki, 1988). Our previous study demonstrated that adlay seed extracts affect testosterone excretion by Leydig cells and that adlay extracts have positive regulatory effects on testosterone secretion, suggesting that adlay components could inhibit androgenic hormone secretion (Hsia et al, 2009). Additionally, adlay can regulate blood sugar homeostasis.…”

Potent <i>in vitro</i> Anti-mouth Cancer (KB) and Immunostimulating Activities of the Job’s Tears (<i>Coix lachryma-jobi</i> Linn.) Seed Semi-purified Extract Cocktails Containing Linoleic Acid

“…1 showed the highest linoleic acid content of 31. 16 . It has been reported that the anti-cancer action of linoleic aicd LA is due to the enhancement of ROS generation and the decrease of cell antioxidant capacity resulting in the mitochondrial damage 14 .…”